Cryopreserving solution for clinical-grade CAR-T cryopreservation and capable of being directly reinfused through intravenous infusion
A technology for cryogenic preservation and cryopreservation, which is applied in the fields of biotechnology applications and medicine, can solve the problems of unstable preservation effect, large batch differences, complex components, etc., and achieves the effects of long shelf life, convenient operation and simple preparation.
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Embodiment 1
[0023] A cryopreservation solution for CAR-T clinical grade cryopreservation and direct intravenous injection reinfusion, comprising the following components: CryoSure-DMSO dimethyl sulfoxide (USP clinical grade) 5.5% (v / v); glycerol Fructose Sodium Chloride Injection 2% (v / v); Invert Sugar Electrolyte Injection 37% (v / v); Dextran 40 Glucose Injection 6% (v / v); Hydroxyethyl Starch 130 / 0.4 Electrolyte Injection 10% (v / v); vitamin C injection 1.2% (v / v); human albumin injection 35% (v / v); 0.9% sodium chloride injection 3.3% (v / v). Purchase the above-mentioned components, mix them in proportion in a medical safety cabinet, freeze and store CAR-T cells for 48 hours according to this formula, and then thaw the cells to obtain a recovery survival rate of more than 62%.
Embodiment 2
[0025] A cryopreservation solution for CAR-T clinical grade cryopreservation and direct intravenous injection reinfusion, including the following components: CryoSure-DMSO dimethyl sulfoxide (USP clinical grade) 7.5% (v / v); glycerol Fructose Sodium Chloride Injection 0% (v / v); Invert Sugar Electrolyte Injection 37% (v / v); Dextran 40 Glucose Injection 6% (v / v), Hydroxyethyl Starch 130 / 0.4 Electrolyte Injection 10% (v / v); vitamin C injection 1.2% (v / v); human albumin injection 35% (v / v); 0.9% sodium chloride injection 3.3% (v / v). Purchase the above-mentioned components, mix them in proportion in a medical safety cabinet, freeze and store CAR-T cells for 48 hours according to this formula, and then thaw the cells to obtain a recovery survival rate of more than 80%.
Embodiment 3
[0027] A cryopreservation solution for CAR-T clinical grade cryopreservation and direct intravenous injection reinfusion, including the following components: CryoSure-DMSO dimethyl sulfoxide (USP clinical grade) 7.5% (v / v); glycerol Fructose Sodium Chloride Injection 0% (v / v); Invert Sugar Electrolyte Injection 37% (v / v); Dextran 40 Glucose Injection 6% (v / v); Hydroxyethyl Starch 130 / 0.4 Electrolyte Injection 10% (v / v); vitamin C injection 1.4% (v / v); human albumin injection 35% (v / v); 0.9% sodium chloride injection 3.1% (v / v). Purchase the above-mentioned components, mix them in proportion in a medical safety cabinet, freeze and store CAR-T cells according to this formula for 48 hours, and then thaw the cells to obtain a recovery survival rate of more than 71%.
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