Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Blood leucocyte separating medium and preparation method, vacuum blood collection tube as well as application thereof

A technology of vacuum blood collection tube and production method, which is applied in the field of blood leukocyte separation liquid and its production, vacuum blood collection tube and its application, can solve the problems of difficult operation, strong professionalism, low collection rate of target cells, etc., and achieve standardization and repetition Good stability, strong quality controllability, and high cell collection rate

Active Publication Date: 2012-10-03
安徽信灵检验医学科技股份有限公司
View PDF4 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In today's medical clinical tests, blood virus gene tests and immune cell function tests all require the isolation of nucleated leukocytes or immune lymphocytes with the ability to phagocytize viruses after blood collection. Traditional cell separation methods generally use the density of various cells Different, prepare cell separation fluid with high ionic strength of the same density of various target cells, add quantitatively to the test tube, slowly add anticoagulant blood into the tube along the tube wall (cannot break through the liquid surface layer of the separation fluid), and then use a centrifuge to centrifuge (The requirement for the range of centrifugal force is very narrow), the centrifugal force and centrifugal radius, and the actual speed of the centrifuge are only theoretically derived values, which cannot be measured and controlled in practice. The professionalism is very strong, the human factors are large, and the repeatability of the separation effect is poor.
[0003] Based on the many defects of the above-mentioned traditional cell separation methods: strong professionalism and difficult operation, there is no unified reagent and operating procedure, the collection rate of target cells is very low, time-consuming and labor-intensive, and repeated procedures are often required. collection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Blood leucocyte separating medium and preparation method, vacuum blood collection tube as well as application thereof
  • Blood leucocyte separating medium and preparation method, vacuum blood collection tube as well as application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0017] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0018] The purpose of the present invention is to solve the problem of separating leukocyte samples with a certain concentration required in blood for clinical medical examination, and to simplify and standardize this complicated and highly specialized separation process.

[0019] The vacuum blood collection tube for blood leukocyte separation provided by the preferred embodiment of the present invention includes a vacuum tube body and a blood leukocyte separation liquid contained in the vacuum tube body. In this embodiment, the tube body of the vacuum tube body is large and long. According to the amount of target cells req...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
lengthaaaaaaaaaa
Login to View More

Abstract

The invention discloses a blood leucocyte separating medium and application thereof, a preparation method of the blood leucocyte separating medium, and a blood leucocyte separating vacuum blood collection tube with the blood leucocyte separating medium and application thereof. The blood leucocyte separating medium comprises glucan T500, hydroxyethyl starch, meglumine diatrizoate, water, sodium citrate, NaN3, poly(diallyldimethylammonium chloride) and polyvinyl pyrrolidone, the proportion of parts by weight of which is 18.5-21.5:1.5-2.5:2-3:69:3.8:0.1:0.05-0.15:2-3. The invention has the advantages that clinical blood collection and anticoagulant leucocyte seperation can be completed at a time, the sodium citrate in the blood leucocyte separating medium and calcium ions in blood are chelate and anticoagulant, the technical problem of damaging the liquid level layer of the separating medium does not exit, a supernatant liquor is sucked up without needing centrifugation after natural precipitation, a complex cell seperation technology is transformed into a simple blood collection seperation process, the processes are easily united, the repeatability of test results is good, and the quality controllability is strong.

Description

technical field [0001] The invention relates to a vacuum container for blood collection and white blood cell density natural sedimentation separation collection in the field of medical clinical examination, in particular to a blood leukocyte separation liquid, an application of the blood leukocyte separation liquid, a preparation method of the blood leukocyte separation liquid, and a blood leukocyte separation liquid The blood leukocyte separation vacuum blood collection tube and the application of the blood leukocyte separation vacuum blood collection tube. Background technique [0002] In today's medical clinical tests, blood virus gene tests and immune cell function tests all require the isolation of nucleated leukocytes or immune lymphocytes with the ability to phagocytize viruses after blood collection. Traditional cell separation methods generally use the density of various cells Different, prepare cell separation fluid with high ionic strength of the same density of v...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/02C12Q1/06C12M1/24
Inventor 王之侃
Owner 安徽信灵检验医学科技股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products