Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Hyla simplex skin injury repair promotion polypeptides and genes as well as application thereof

A technology for skin damage and hyla, which is applied in the field of biomedicine to achieve the effect of promoting proliferation and tissue regeneration, with simple structure and promoting proliferation.

Inactive Publication Date: 2011-11-23
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
View PDF1 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The application of amphibian drugs has a long history in my country, but the research on their active ingredients and pharmacological properties mainly focuses on small organic molecules such as alkaloids, and there are few researches on their skin active protein peptides

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Hyla simplex skin injury repair promotion polypeptides and genes as well as application thereof
  • Hyla simplex skin injury repair promotion polypeptides and genes as well as application thereof
  • Hyla simplex skin injury repair promotion polypeptides and genes as well as application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0043] The substantive content of the present invention is further described below with embodiment, but content of the present invention is not limited thereto.

[0044] Cloning of peptide gene clone of hyla hyla to promote skin damage repair:

[0045] I. Total RNA Extraction from Hyla Hyla Skin:

[0046] A. The living body hyla hyla was cleaned with water, put into liquid nitrogen and quick-frozen for 4 hours, took skin tissue, weighed, got 300mg skin tissue, added 10ml total RNA extraction buffer solution (Trizol solution, product of U.S. GIBCOBRL company), in 20ml glass Homogenize in a homogenizer for 30 minutes.

[0047] B. Add an equal volume of phenol / chloroform solution, mix vigorously, leave at room temperature for 10 minutes, centrifuge at 12,000 rpm for 10 minutes at 4°C, and discard the precipitate.

[0048] C. Add an equal volume of isopropanol to the supernatant, leave it at room temperature for 10 minutes, centrifuge at 12,000 rpm at 4°C for 10 minutes, wash th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to hyla simplex skin injury repair promotion polypeptides and genes as well as application thereof, and belongs to the field of biomedicine. The hyla simplex skin injury repair promotion polypeptides comprise Hylareleasin 3 and Hylareleasin 4, which are gene codes of hyla simplex, a Chinese amphibious animal, wherein the molecular weights of the Hylareleasin 3 and 4 are 1764.106 Daltons and 1707.05 Daltons respectively; the isoelectric points are 9.61 and 9.60 respectively; the complete sequences of the hyla simplex skin injury repair promotion polypeptides Hylareleasin 3 and Hylareleasin 4 are NH2-GLLDPVTHILGGFLRR-COOH and NH2-GLLDPVTNILGGLLRR-COOH respectively; the genes coded by both the polypeptides consist of 365 nucleotides; and the mature coding part comprises the nucleotides 217-264. The multiplication of human umbilical vein endothelial cells (HUVEC) and the activity of the HUVEC for releasing epidermal growth factor (EGF) and vascular endothelial growth factor (VEGF) are promoted by the synthetic hyla simplex skin injury repair promotion polypeptides; and the hyla simplex skin injury repair promotion polypeptides can be applied to preparing preparations for promoting multiplication and tissue regeneration of vascular endothelial cells and epidermic cells and treating wounds, burns and ulcers of the body surface. The hyla simplex skin injury repair promotion polypeptides also have the advantages of simple sequences and convenience for synthesis.

Description

Technical field: [0001] The invention provides two Hyla simplex skin damage-promoting and repairing polypeptides, Hylareleasin3 and Hylareleasin4, their genes and applications, and belong to the technical field of biomedicine. Background technique: [0002] As the body's natural protective barrier, the skin has the function of mechanically blocking pathogens. Once the natural barrier function of the skin is damaged, the body will immediately and automatically turn on the physiological switch to repair the damage of the skin. Skin injury repair consists of a series of complex biochemical and cell biological events. The classic skin injury repair model is divided into four consecutive or overlapping stages: hemostasis, inflammation, proliferation and remodeling. Therefore, drug development targeting one or several stages of the skin injury repair process may have good targeted therapeutic significance. [0003] In the proliferative phase of skin injury repair, angiogenesis i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K7/08C12N15/12A61K38/10A61P17/02
Inventor 赖仞武静刘涵
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com