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Application of tanshinol derivatives in preparation of drug for preventing cardiovascular-related diseases

A Danshensu derivative, cardiovascular prevention and treatment technology, applied in the direction of cardiovascular system diseases, drug combinations, pharmaceutical formulations, etc., can solve the problems that are not suitable for large-scale promotion and use, time-consuming sequencing equipment and high cost of reagents

Inactive Publication Date: 2013-09-11
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The current method for detecting this point mutation is mainly sequencing after polymerase chain reaction (PCR), but the sequencing in this method is time-consuming and requires high equipment and reagent costs, which is not suitable for large-scale promotion and use

Method used

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  • Application of tanshinol derivatives in preparation of drug for preventing cardiovascular-related diseases
  • Application of tanshinol derivatives in preparation of drug for preventing cardiovascular-related diseases
  • Application of tanshinol derivatives in preparation of drug for preventing cardiovascular-related diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Danshensu Derivatives (1) Protective Effects on Human Umbilical Vein Endothelial Cells

[0048] Grouping: Divide 1×10 5 HUVEC cells were seeded into a 6cm culture dish and cultured for 2 days to reach 70-80%, and the cells were synchronized for 4-8 hours. They were randomly divided into 6 groups, namely: ① normal control group; ② model group; low-dose Danshensu derivative group; ④ Middle dose group of Danshensu derivatives; ⑤ High dose group of Danshensu derivatives; ⑥ Positive drug group. Administration: ③-⑤Add danshensu derivatives at final concentrations of 5μM, 50μM, 100μM, ⑥NAC 5mM, incubate for 4h. Join H 2 o 2 Culture induces oxidative stress in endothelial cells. Cell apoptosis was measured by flow cytometry.

[0049] Experimental result proves, described Danshensu derivative (1) to H 2 o 2 The induced HUVEC cell apoptosis has obvious inhibitory effect, and the medicine for preventing and treating cardiovascular related diseases can be prepared....

Embodiment 2

[0050] Example 2 Danshensu derivatives (1) protect human umbilical vein endothelial cells

[0051] Grouping: Divide 1×10 5 HUVEC cells were seeded into a 6cm culture dish and cultured for 2 days to reach 70-80%, and the cells were synchronized for 6-12 hours. They were randomly divided into 6 groups, namely: ① normal control group; ② model group; ③ low-dose Danshensu derivative group; ④ Middle dose group of Danshensu derivatives; ⑤ High dose group of Danshensu derivatives; ⑥ Positive drug group. Administration: ③-⑤ Add Danshensu derivatives at final concentrations of 5 μM, 50 μM, 100 μM, ⑥ NAC 5 mM, and incubate for 4 hours. Join H 2 o 2 Culture induces oxidative stress in endothelial cells. The total protein was extracted, and the expression of apoptosis-related genes was determined by Western-blot. Apoptosis-related genes determined: Bax, Bcl-2, PARP. The experimental data were analyzed by one-way analysis of variance, and P<0.05. The results showed that Danshensu deri...

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Abstract

The invention belongs to the field of Chinese medicine production and relates to application of tanshinol derivatives in Chinese herbal medicine salvia in preparation of a pharmaceutical composition for preventing cardiovascular-related diseases. According to the in-vitro oxidation stress model experiment research, the result shows that the tanshinol derivatives can be used for preventing the cardiovascular-related diseases by protecting endothelial cells of a circulatory system. More specifically, the tanshinol derivatives can be used for achieving the effect of protecting endothelial cells by restraining transcriptional level of the cardiovascular-related diseases, restraining protein expression level of the cardiovascular-related diseases, restraining the production of reactive oxygen species and restraining the corresponding signal transduction pathway and transcription factor. According to the experimental result, the tanshinol derivatives have an obvious inbibitional effect on apoptosis of HUVEC (Human Umbilical Vein Endothelial Cells) induced by H2O2 and have an obvious protection effect on cells damaged by oxidative stress, and can be used for further preparing a drug for preventing cardiovascular-related diseases.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine pharmacy, and relates to the application of derivatives of Danshensu, a specific monomer in the Chinese herbal medicine Salvia miltiorrhiza, in the preparation of pharmaceutical compositions, especially the application in the preparation of pharmaceutical compositions for preventing and treating cardiovascular-related diseases. Background technique [0002] Cardiovascular diseases are also known as circulatory system diseases. Usually, the circulatory system refers to the organs and tissues that transport blood in the human body, mainly including the heart and blood vessels (arteries, veins, microvessels). Circulatory system diseases can be subdivided into acute and chronic, generally associated with arteriosclerosis. [0003] The prior art discloses that the salvia miltiorrhiza is the dry root and rhizome of the dicot plant Salvia miltiorrhiza Bge. From "Shen Nong's Materia Medica", i...

Claims

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Application Information

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IPC IPC(8): A61K31/216A61P9/00
Inventor 朱依谆王洋郑洪明董晓易贾尧玲
Owner FUDAN UNIV
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