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Method for manufacturing three-dimensional small-diameter vessel model

A vascular model and small-caliber technology, applied in the field of bioengineering, can solve the problems of high operation difficulty, complicated production steps, and many technological processes, and achieve the effect of good biocompatibility and strong mechanical properties.

Inactive Publication Date: 2018-09-07
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, existing patent documents such as CN201510355045.5, CN201510953566.0, CN201611189284.9, etc., disclose the preparation method of various cell-stent three-layer structure small-caliber vascular stents, but due to the combination of multiple technologies, the process flow is relatively complicated. Many, the production steps are complicated, the actual operation is difficult, and it cannot be produced at one time

Method used

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  • Method for manufacturing three-dimensional small-diameter vessel model
  • Method for manufacturing three-dimensional small-diameter vessel model
  • Method for manufacturing three-dimensional small-diameter vessel model

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Embodiment Construction

[0051] Embodiments of the present invention will be described in detail below. It should be emphasized that the following description is only exemplary and not intended to limit the scope of the invention and its application.

[0052] refer to Figure 1 to Figure 7 , in one embodiment, a method for making a three-dimensional small-diameter blood vessel model, comprising the following steps:

[0053] a. Preparation of support bracket: use silicone material to 3D print a support bracket with inner and outer concentric circular structures;

[0054] b. Formation of the media: perfusing the decellularized extracellular matrix containing human aortic smooth muscle cells in the middle layer of the support bracket to form the media;

[0055] c, intima formation: perfuse the human umbilical vein endothelial cell suspension in the inner layer of the support bracket to form the intima;

[0056] d. Adventitia formation: placing the support bracket as a whole into a dynamic culture syst...

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Abstract

The invention discloses a method for manufacturing a three-dimensional small-diameter vessel model. The method comprises the following steps of a, preparation of a supporting bracket: utilizing a silica gel material to perform 3D printing of a supporting bracket with an inner and outer concentric circle shape structure; b, formation of a medial membrane: pouring an accellular extracellular matrixcontaining a human aortic smooth muscle cell into a middle layer of the supporting bracket to form the medial membrane; c, formation of an intima: pouring a human umbilical vein endothelial cell suspension into an inner layer of the supporting bracket to form the intima; and d, formation of an adventitia: wholly putting the supporting bracket in a dynamic culture system fully filled with human newbaby dermal fibroblast suspension to form the adventitia. The vessel model acquired with the method is good in biocompatibility, has relatively strong mechanical property and can be used for pathological model study and drug screening relevant to vessels.

Description

technical field [0001] The invention relates to bioengineering technology, in particular to a method for making a three-dimensional small-diameter blood vessel model. Background technique [0002] At present, cardiovascular disease is one of the main causes of human death. Due to the limited source of human autologous blood vessels, the construction of in vitro models of vascular-related diseases is an effective method for its research. With the development of bio-3D printing technology, through the combination of computer technology, engineering and materials science, it is possible to construct tissue-engineered blood vessels for the construction of in vitro blood vessel-related models. Vascular tissue engineering technology provides a new source for small-caliber blood vessel models, and its idea is to use the combined culture of vascular cells and scaffolds to construct biologically active and functional small-caliber blood vessel analogs. [0003] At present, many stra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/50A61L27/40A61L27/36A61L27/38A61L27/56B33Y10/00B33Y80/00B33Y70/00
CPCA61L27/3633A61L27/3687A61L27/3804A61L27/3808A61L27/3826A61L27/3895A61L27/50A61L27/507A61L27/56B33Y10/00B33Y70/00B33Y80/00
Inventor 弥胜利孙伟胡颖英姚弘毅苏鑫
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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