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Depolymerized holothurian glycosaminoglycan composition as well as preparation method and application thereof

A technology of glycosaminoglycan and composition, which is applied in the application field of depolymerized sea cucumber glycosaminoglycan composition in the prevention and treatment of tumor diseases, and achieves the effects of reasonable preparation process design, improved degradation method, and strong operability

Active Publication Date: 2017-01-25
SUZHOU YI HUA BIOMEDICAL TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the application of dHG in anti-tumor at home and abroad.

Method used

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  • Depolymerized holothurian glycosaminoglycan composition as well as preparation method and application thereof
  • Depolymerized holothurian glycosaminoglycan composition as well as preparation method and application thereof
  • Depolymerized holothurian glycosaminoglycan composition as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: Extraction and purification of sea cucumber glycosaminoglycan (HG)

[0034] 1. Weigh 5Kg of dried Yuzu sea cucumber medicinal material and soak in water overnight. Wash, take body wall, drain water, mince, weigh and rehydrate to 25Kg. Place in a water bath at 60°C, adjust the pH to 8.0 with sodium hydroxide, add 2% alkaline protease 2709, and after 6 hours of enzymolysis, add 2% trypsin to continue the enzymolysis for 16 hours. During the enzymolysis process, pay attention to controlling the pH to Weakly alkaline. Centrifuge after enzymolysis, add an equal volume of ethanol to the supernatant, collect the solid by centrifugation, dissolve it with 30 times the mass of water, add 1% NaCl solid to dissolve it completely, and then add 1Kg (wet weight) 1074 type Ion exchange resin, adjust the pH to 8.0, stir and adsorb at room temperature for 4 hours, after the adsorption is completed, filter with suction, wash the resin after the suction with water first, th...

Embodiment 2

[0036] Embodiment 2: Preparation of depolymerized sea cucumber glycosaminoglycan (dHG)

[0037] 1. Preparation of dHG

[0038] Take 20 g of the pure sea cucumber glycosaminoglycan prepared in Example 1, add 160 mL of water to dissolve, add 19.0 mL of acetic acid and 60.0 mL of hydrogen peroxide, stir and react at 60°C for 22 hours, cool in an ice bath, and then add Adjust the pH to 9.5 with 4M NaOH solution, then add 2 times the volume of ethanol for ethanol precipitation, let stand, and centrifuge to obtain a precipitate, which is the crude product of depolymerized sea cucumber glycosaminoglycan.

[0039] Take the crude product and dissolve it in 200mL of water, add 3% NaCl solid, ethanol precipitation with 1 volume of ethanol, redissolve the obtained precipitate, and then use 2%, 1% NaCl solid and 1 volume of ethanol precipitation twice respectively to finally get The precipitate was washed twice with ethanol, and then dried under reduced pressure to obtain 18.0 g of depoly...

Embodiment 3

[0052] Example 3: Acute toxicity test of depolymerized sea cucumber glycosaminoglycan dHG

[0053] 1. Experimental materials

[0054] dHG10092 (prepared according to the method of Example 2 of the present invention); ICR mice.

[0055] 2. Experimental method

[0056] The ICR mice were randomly divided into two groups according to body weight (n=10 in each group, half male and half male), and were given dHG10092 at doses of 4000 mg / kg and 2000 mg / kg by tail vein respectively.

[0057] 3. Experimental results

[0058] Some animals in the 4000mg / kg dose group immediately appeared convulsions, dyspnea, abdominal breathing, loss of righting reflex, and urinary incontinence after administration of the tail vein. Significantly less than

[0059] Animals in the 2000mg / kg dose group had no obvious abnormal symptoms after tail vein administration. After the administration, the body weight of the animals in each group decreased, and there was a significant difference (P2 After the a...

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Abstract

The invention discloses a depolymerized holothurian glycosaminoglycan composition, as well as a preparation method and application of the depolymerized holothurian glycosaminoglycan composition. The depolymerized holothurian glycosaminoglycan composition is one or more of depolymerized holothurian glycosaminoglycan with the weight-average molecular weight between 2000Da and 12000Da, and the preparation method of the depolymerized holothurian glycosaminoglycan composition comprises extracting and purifying holothurian glycosaminoglycan, degrading the holothurian glycosaminoglycan and the like. Screened through a plurality of experiments, echinodermata holothurian is chosen as an object of study, and an extracting and purifying process of the holothurian glycosaminoglycan and a degrading process of the depolymerized holothurian glycosaminoglycan are chosen from a large quantity of experiments. The whole process is reasonable in design and strong in operability, and the industrial production is implemented. The anti-tumor study shows that the depolymerized holothurian glycosaminoglycan composition remarkably inhibits the generation of a lumen of a human umbilical vein endothelial cell in vitro, inhibits the transfer of melanin and a breast cancer in vivo, has an excellent anti-tumor effect, is expected to be developed into an efficient antitumor drug or healthcare product with low toxic or side effects, and has an important application prospect.

Description

technical field [0001] The invention belongs to the technical field of medicine and health care products, and the invention relates to a depolymerized sea cucumber glycosaminoglycan composition with a weight-average molecular weight range of 2000Da-12000Da and a preparation method thereof, and the depolymerized sea cucumber glycosaminoglycan composition in the prevention and treatment of Application in the treatment of tumor diseases. Background technique [0002] Malignant tumor is a common disease that seriously threatens human life. At present, the main treatment methods are surgery, radiotherapy, chemotherapy and immunotherapy. Chemotherapy, one of the treatment methods, has two major obstacles while inhibiting and killing tumor cells, namely its toxic side effects and drug resistance. Toxic and side effects are reflected in not only killing tumor cells, but also killing normal tissue cells, causing toxic and side effects to the human body and reducing the physiologica...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/726A61P35/00A23L1/09
Inventor 朱莹莹金永生王林林陈武姚亦明蒋毅
Owner SUZHOU YI HUA BIOMEDICAL TECH CO LTD
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