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Method for immobilized culture of salt-tolerant bacillus strains and application

A technology of immobilized cultivation of halotolerant bacillus, applied in chemical instruments and methods, methods based on microorganisms, biochemical equipment and methods, etc., to achieve the effect of not easy to lose, obvious effect, and remarkable effect

Inactive Publication Date: 2016-09-28
JIANGSU RUIDA TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Bacillus sp. strain is a facultative anaerobic bacterial strain, which is widely used in wastewater treatment of environmental engineering, including extracting microbial flocculants, degrading dyes, heavy metals and organic pollutants; however, using a new immobilized culture method And under the condition of high salt concentration, it can effectively degrade various pollutions, which has not been mentioned at home and abroad.

Method used

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  • Method for immobilized culture of salt-tolerant bacillus strains and application
  • Method for immobilized culture of salt-tolerant bacillus strains and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1.1. Screening of halotolerant bacillus strains:

[0035] (1) Get 15g of soil samples from sun-dried salt fields, and place them in 150ml of high-salt wastewater containing azo dyes, potassium dichromate, and phenol for anaerobic treatment for 8 days;

[0036] (2) Get 8ml of the waste water that above-mentioned anaerobic treatment has finished, inoculate in the 150ml high-salt waste water that contains azo dyestuff, potassium dichromate and phenol respectively in fresh configuration, anaerobic treatment again 8 days;

[0037] (3) Repeat step (2) 4 times; Get 80 μ l of waste water that has been treated for the last time and evenly spread it on the activated plate medium, and after 24 hours of growth, mark the single colony on the plate medium;

[0038] (4) Pick a single colony on the culture medium, inoculate it into the enrichment medium for aerobic growth for 24 hours, and then place all of them in 150ml of high-salt wastewater containing azo dyes for anaerobic treatme...

Embodiment 2

[0047] Embodiment 2: The halotolerant Bacillus bacillus loaded with magnetic nanoparticles cultivated in embodiment 1 is applied to the treatment of high-salt wastewater containing azo dyes. The specific process is as follows:

[0048] Put the halotolerant Bacillus strain loaded with magnetic nanoparticles with a concentration of 50-300mg / L into the synthetic wastewater with a salt concentration of 15% and a methyl red concentration of 500mg / L at a volume ratio of 1.5% for degradation Effect test, put the whole system in an anaerobic reactor, pass nitrogen into the anaerobic reactor for 5 minutes, seal it and place it in a low-speed shaker for shaking culture. Set the shaking speed at 50rpm, take appropriate amount of liquid after 1 hour, 2 hours and 6 hours of degradation, separate by magnetic field, centrifuge, and then take the supernatant to read at 420nm in the spectrophotometer. This embodiment is divided into five groups of parallel experiments, and the degradation temp...

Embodiment 3

[0051] Embodiment 3: the halotolerant bacillus bacillus that is loaded with magnetic nanoparticles cultivated in embodiment 1 is applied in the treatment of high-salt wastewater containing potassium dichromate, and the specific process is as follows:

[0052] Put the halotolerant Bacillus strain loaded with magnetic nanoparticles with a concentration of 50-300mg / L into the synthetic wastewater with a salt concentration of 15% and a potassium dichromate concentration of 150mg / L at a volume ratio of 1% for degradation Effect test, put the whole system in an anaerobic reactor, pass nitrogen gas into the anaerobic reactor for 5 minutes, seal it, place it in a low-speed shaker for shaking culture. Set the shaking speed at 50 rpm, take appropriate amount of liquid every 8 hours, undergo magnetic field separation, centrifuge, and then take the supernatant for analysis and measurement by atomic absorption spectrophotometer and chemical titration. This embodiment is divided into five g...

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Abstract

The invention belongs to the technical field of industrial wastewater treatment, and particularly relates to a method for immobilized culture of salt-tolerant bacillus strains. The method mainly comprises the following steps that salt-tolerant bacillus strains are inoculated in a salt-tolerant culture medium for activated growth; the salt-tolerant bacillus strains obtained after activated growth are inoculated on an activated plate culture medium for culture; the strains obtained after being cultured on the activated plate culture medium are inoculated to a salt-tolerant culture medium containing magnetic nanometer materials for shake culture; standing and separation of a culture medium system are performed in a magnetic field, and salt-tolerant bacillus strain precipitations loaded with magnetic nanometer particles are centrifuged and collected. The salt-tolerant bacillus strains loaded with magnetic nanometer particles can be applied to industrial wastewater treatment and have the advantages of being easily separated from wastewater, not likely to be lost, easy to use, low in cost and high in degradation efficiency.

Description

technical field [0001] The invention belongs to the technical field of industrial wastewater treatment, and in particular relates to an immobilized culture method and application of a halotolerant bacillus strain. Background technique [0002] Azo dye wastewater, heavy metal Cr(VI) wastewater and phenol wastewater are the more common types of industrial wastewater. It is characterized by poor biodegradability, high salinity, and large discharge. The pollution sources mainly include cosmetics factories, metallurgical factories, chemical factories, textile factories, electroplating factories, etc. If they are discharged without effective treatment, they will spread through water and soil, causing serious pollution. Threat to the animals, plants and human health in the area. [0003] In addition, during the discharge of such industrial wastewater, it often contains high concentrations of salt, and the more common salt concentration ranges from 5 to 15%. It is generally believ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C02F3/34C02F3/02C02F3/28C12R1/07C02F101/22C02F101/38C02F101/34C02F101/20
CPCC02F3/02C02F3/28C02F3/34C02F2101/20C02F2101/22C02F2101/345C02F2101/38C12N11/14Y02W10/10
Inventor 付博虞磊周乔刘立清王立文高静静
Owner JIANGSU RUIDA TECH CO LTD
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