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Culture solution for bacillus, preparation method and application thereof

A Bacillus and Bacillus subtilis technology, applied in the field of microbial fertilizers and microbial pesticides, can solve the problems of reduced use effect, increased production cost, reduced number of effective viable bacteria, etc., and achieves improved use effect, improved use effect, and reduced production costs. Effect

Inactive Publication Date: 2017-06-20
安徽棵康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the use of bacillus inoculum generally adopts the recovery of spores after fermentation, and is prepared into various dosage forms for use. On the one hand, the biologically active substances that are effective against diseases and insect pests produced in the fermentation liquid are discarded, and the germination of spores takes a certain time, which is different from that of on-site fermentation. Compared with microbial agents, the use effect is greatly reduced; and the number of effective viable bacteria is also reduced during processing, storage and use, and the post-treatment process increases production costs

Method used

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  • Culture solution for bacillus, preparation method and application thereof
  • Culture solution for bacillus, preparation method and application thereof
  • Culture solution for bacillus, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Preparation method and application of bacillus amyloliquefaciens microbial agent

[0032] 1. The preparation method is as follows:

[0033] Clean the equipment before culturing, and clean the fermenter and its accessories with tap water until there is no culture residue. Take 2.00% soluble starch, 1.00% soybean protein powder, 0.50% yeast powder, 0.30% KH 2 PO 4 , 0.05% MgSO 4 ·7H 2 O, 0.02% MnSO 4 ·H 2 O, 0.08% NaOH was added to the expansion tank to prepare the basic culture solution, and then 1.00‰ defoamer was added to replenish the water to 140L. Heat the culture solution to 100°C and heat-treat for 30-60 minutes, then cool down to 80°C, add 1.00% Bacillus amyloliquefaciens seeds to the culture solution and heat-treat for 30 minutes, then ventilate and cool down to 35°C.

[0034] The above-mentioned obtained liquid is cultivated with air, and the culture conditions are about 0.8-1.0vvm ventilation, 32°C temperature, and 36-48h culture time, and the culture l...

Embodiment 2

[0041] Preparation method and application of Bacillus subtilis microbial agent

[0042] 1. The preparation method is as follows:

[0043] Clean the equipment before expanding the cultivation, and clean the hot water tank and its accessories with tap water until there is no culture residue. Pass corn starch and soybean powder through a 60-mesh sieve, take 1.50% corn starch, 0.50% soybean powder, 0.50% corn syrup powder, and 0.20% KH 2 PO 4 , 0.03% MgSO 4 ·7H 2 O, 0.01% MnSO 4 ·H 2O, 0.10% NaOH was added to the expansion tank to prepare the basic culture solution, and then 1.00‰ defoamer was added to replenish the water to 140L. Heat the culture solution to boiling, add 1.00wt‰ Bacillus subtilis seeds into the culture solution for heat treatment for 30 minutes, then ventilate and cool down to 42°C.

[0044] The above-mentioned obtained liquid is cultivated with air, and the culture conditions are about 1.0vvm ventilation, 30-37°C temperature, and 36h culture time. The cul...

Embodiment 3

[0051] Preparation method and application of bacillus licheniformis microbial agent

[0052] 1. The preparation method is as follows:

[0053] Clean the equipment before the expansion, and clean the expansion tank and its accessories with tap water until there is no culture residue. Pass the peanut powder through a 60-mesh sieve, weigh 2.00% sucrose, 1.00% peanut powder, 0.30% yeast extract, 0.30% KH 2 PO 4 , 0.04% MgSO 4 ·7H 2 O, 0.02% MnSO 4 ·H 2 O, 0.07% NaOH was added to the expansion tank to prepare the basic culture solution, then 1.00‰ defoamer was added to the expansion tank, and the water was replenished to 400L. After heating the culture solution to 80°C, take 0.50% Bacillus licheniformis inoculum and add it to the culture solution for 20 minutes, then ventilate and cool down to 42°C.

[0054] The liquid obtained above was cultivated with air under the conditions of ventilation of about 0.5-1.0vvm, temperature of 32°C, and incubation time of 24 hours, and the ...

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Abstract

The invention discloses a culture solution for bacillus. The culture solution comprises the following components by mass percentage: 1.50-3.00% of a carbon source, 0.30-1.00% of a nitrogen source, 0.10-0.50% of a yeast extract or corn steep liquor powder or peptone, 0.10-0.30% of K2HPO4 or KH2PO4, 0.02-0.05% of MgSO4.7H2O, 0.01-0.02% of MnSO4.H2O, 0.05-0.10% of NaOH, 1.00-2.00 per thousand of a defoaming agent, 0.10%-1.00% of bacillus seed, and the balance water. The invention also discloses a preparation method of the culture solution and application of the culture solution in control of plant diseases and insect pests. Under a non-sterile airtight condition, the culture solution provided by the invention can realize on-the-spot small scale enlarged culture, the use effect is improved, and also the production cost is lowered.

Description

technical field [0001] The invention belongs to the technical field of microbial fertilizers and microbial pesticides, and in particular relates to a culture solution for bacillus-like microbial agents. The invention also relates to a preparation method of the bacillus culture solution and its application in the control of plant diseases and insect pests. Background technique [0002] Extensive use of chemical pesticides has resulted in pesticide residues in food and environmental pollution, which has seriously threatened human health. In addition, the use of large amounts of chemical pesticides can easily cause pests and plant pathogens to develop resistance and reduce the control effect. Biopesticides are considered to be better substitutes for chemical pesticides due to their environmental friendliness, pollution-free, and non-resistance characteristics. [0003] Bacillus is a beneficial microorganism on the leaf surface and rhizosphere of plants, and it is also a common ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07C12R1/125C12R1/10
CPCC12N1/20A01N63/00
Inventor 黄亦存尹春林罗江龙
Owner 安徽棵康生物科技有限公司
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