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Strain for synthesizing gamma-polyglutamic acid and method for efficiently preparing gamma-polyglutamic acid by using strain

A polyglutamic acid strain and the technology of polyglutamic acid are applied in the fields of microorganisms and fermentation, can solve the problems of high production cost, low production efficiency, long production cycle and the like, and achieve the effect of efficient and cheap preparation

Inactive Publication Date: 2014-12-24
樟树市狮王生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the fermentation production of the above γ-polyglutamic acid has made great progress, there are still problems of long production cycle, low production efficiency and high production cost.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A bacterial strain for synthesizing γ-polyglutamic acid, screened from the soil around the fertilizer plant, named Bacillus subtilis NS-9, namely Bacillus Subtilis NS-9, and the preservation number of China Center for Type Culture Collection CCTCC NO: M2014142 , date of deposit April 23, 2014.

[0024] 16s rDNA sequence analysis: Most of the 16s rDNA sequence was measured at 1600bp, and the measured sequences were compared with related species in the GenBank database to construct a phylogenetic tree based on the full 16s rDNA sequence. The results showed that the homology between the strain and Bacillus Subtilis was 99%. Combined with the phylogenetic research results of morphology, cytochemical composition and 16s rDNA full-series analysis, it can be classified as Bacillus subtilis, specifically Bacillus subtilis (Bacillus Subtilis) NS-9.

Embodiment 2

[0026] Utilize the synthetic bacterial strain of the gamma-polyglutamic acid of embodiment 1 to efficiently prepare the method for gamma-polyglutamic acid, the steps are as follows:

[0027] (1) Slant culture: Inoculate the cryopreserved strain of Example 1 on the slant medium for activation, and culture at 37°C for 20 hours. The components of the slant medium: peptone 10g / L, yeast powder 5g / L, sodium chloride 10g / L , agar powder 15g / L, pH value 7.0;

[0028] (2) Seed culture: inoculate the activated strain in a sterile seed medium containing carbon source, nitrogen source, glutamate, inorganic salt and water, and cultivate at 37°C for 12 hours to logarithmic Mid-growth stage; the composition of the seed medium is peptone 10g / L, yeast powder 5g / L, sodium chloride 10g / L, pH 7.0, and sterilized at 121°C for 20 minutes;

[0029] (3) Fermentation culture: put the seed solution obtained in step (2) into a sterile shake flask fermentation medium containing carbon source, nitrogen s...

Embodiment 3

[0035] Utilize the synthetic bacterial strain of the gamma-polyglutamic acid of embodiment 1 to efficiently prepare the method for gamma-polyglutamic acid, the steps are as follows:

[0036] (1) Slant culture: Inoculate the cryopreserved strain of Example 1 on the slant medium for activation, and culture at 37°C for 20 hours. The components of the slant medium: peptone 10g / L, yeast powder 5g / L, sodium chloride 10g / L , agar powder 15g / L, pH value 7.0;

[0037] (2) Seed culture: inoculate the activated strain in a sterile seed medium containing carbon source, nitrogen source, glutamate, inorganic salt and water, and cultivate at 37°C for 12 hours to logarithmic Mid-growth stage; the composition of the seed medium is peptone 10g / L, yeast powder 5g / L, sodium chloride 10g / L, pH 7.0, and sterilized at 121°C for 20 minutes;

[0038] (3) Fermentation culture: put the seed solution obtained in step (2) into a sterile shake flask fermentation medium containing carbon source, nitrogen s...

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Abstract

The invention discloses a strain for synthesizing gamma-polyglutamic acid. The strain is screened from soil around a chemical fertilizer plant and is classified to be named as Bacillus Subtilis NS-9, the collection number of the strain in the China Center for Type Culture Collection (CCTCC NO.) is M2014142, and the collection date of the strain is April 23, 2014. The invention also discloses a method for efficiently preparing gamma-polyglutamic acid by using the strain for synthesizing gamma-polyglutamic acid. Compared with an existing gamma-polyglutamic acid production technique, the adopted bacillus subtilis microbial strain disclosed by the invention can be used for efficiently producing gamma-polyglutamic acid by virtue of fermentation; by optimizing the culture conditions of the bacillus subtilis strain, particularly replenishing a carbon source, inorganic salts and energy substances in a fermentation process, the content of gamma-polyglutamic acid in a fermentation solution can reach 100-200g / L, so that the method for preparing gamma-polyglutamic acid, disclosed by the invention, is high in efficiency and low in cost.

Description

technical field [0001] The invention belongs to the technical field of microorganisms and fermentation, and in particular relates to a synthetic bacterial strain of gamma-polyglutamic acid. The invention also relates to a method for efficiently preparing gamma-polyglutamic acid by using a synthetic bacterial strain of gamma-polyglutamic acid. Background technique [0002] γ-polyglutamic acid (γ-polyglutamic acid, hereinafter referred to as γ-polyglutamic acid) is a kind of homopolyamino acid formed by connecting D-glutamic acid and L-glutamic acid monomers through γ-amide bonds. , can be synthesized by microbial fermentation. Because γ-polyglutamic acid has excellent physical, chemical and biological properties such as film-forming, fibrillating, plasticity, adhesiveness, moisturizing, and degradability, it is extremely useful in the fields of industry, agriculture, cosmetics, and medicine. Has application potential. [0003] The preparation methods of polyglutamic acid i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P13/00C12R1/125
Inventor 洪立芝殷志敏李宗波
Owner 樟树市狮王生物科技有限公司
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