Method for preparing bacteria cellulose compound film and application thereof as face pack material
A technology of bacterial cellulose film and bacterial cellulose, which is applied in the application field of bacterial cellulose composite film as a mask material, can solve the problems of inconvenient use, the performance of the mask being affected by a specific carrier, and the poor ductility of the mask, etc. Water content, low cost, and the effect of improving conversion rate
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Embodiment 1
[0033] (1) Strain selection: Gluconacetobacter xylinum CGMCC No.1.1812 was selected.
[0034] (2) Preparation of polyglutamic acid containing seeds and liquid medium: in weight percentage, containing 2% fructose, 0.5% yeast powder, 0.5% tryptone, 0.5% Na 2 HPO 4 , 0.2% citric acid, 2% calcium carbonate seed medium or liquid medium, add 0.5g / L polyglutamic acid, then adjust the pH value of the medium to 6, and sterilize at a temperature of 121°C for 20min, After cooling to 30°C, set aside;
[0035] (3) Seed cell culture: Take the slant seeds (Gluconacetobacterxylinum (Gluconacetobacterxylinum) CGMCC No.1.1812) that have been activated in the 1-2 rings and insert them into the liquid medium containing polyglutamic acid, shake and cultivate at 30°C for 24 hours, shake The bed speed was 160r / min to obtain the seed liquid;
[0036] (4) Production of bacterial cellulose by static liquid fermentation: Inoculate the seed liquid into the liquid medium containing polyglutamic acid with...
Embodiment 2
[0040] Calculated by weight percentage, take 2% glucose, 0.5% yeast powder, 0.5% tryptone, 0.5% Na 2 HPO 4 , 0.2% citric acid, 2% calcium carbonate, 0.05% polyglutamic acid, adjust the pH to 6.0. Sterilize at 121°C for 20min. Using this as a liquid medium, take a ring of activated slant seed Acetobacter xylinum (Gluconacetobacter xylinum) CGMCC No.1.2378, insert it into the liquid medium, and cultivate it with shaking at 30°C for 24 hours, with the shaker speed at 160r / min, as seed liquid. Inoculate the liquid culture medium (500mL Erlenmeyer flask contains 200mL liquid medium) with 6% inoculum amount, fully shake it when inoculating, so that the bacteria liquid is uniform, and culture at a constant temperature of 30°C for 6 days. The bacterial cellulose film generated after standing at constant temperature for 6 days floated on the liquid surface. After the membrane is taken out, rinse with water several times to remove the culture medium and impurities on the surface of t...
Embodiment 3
[0043] Calculated by weight percentage, take 2% glucose, 0.5% yeast powder, 0.5% tryptone, 0.5% Na 2 HPO 4 , 0.2% citric acid, 2% calcium carbonate, 0.04% polyglutamic acid, adjust the pH to 6.0. Sterilize at 121°C for 20min. Using this as a liquid medium, take a ring of activated slant seeds (Gluconacetobacter xylinum) CGMCC No.1.1812, insert it into the liquid medium, and cultivate it with shaking at 32°C for 24 hours, with the shaker speed at 160r / min, as the seed . Inoculate 7% of the inoculum into the liquid culture medium (distribute into shallow fermentation trays, clean the fermentation trays, and sterilize with ozone water), fully shake when inoculating to make the bacterial liquid uniform, with a thickness of 10mm, at 32°C Constant temperature static culture for 6 days. The bacterial cellulose film generated after standing at constant temperature for 6 days floats on the liquid surface with a thickness of about 8 mm. After the membrane is taken out, rinse with wa...
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