236 results about "Gentamycins" patented technology

The invention relates to an extremely-low sperm freezing protective agent and application thereof. The protective agent is prepared from the following ingredients: sodium chloride, potassium chloride, magnesium sulfate, monopotassium phosphate, calcium chloride, fructose, trehalose, sodium citrate, glycin, glutamine, pentoxifylline, vitamin E, HAS (human serum albumin), glycerin and gentamicin sulphate. The extremely-low sperm freezing protective agent can protect less sperms with low activity and without forward activity ability in seminal fluid, can remarkably improve the activity ability of the sperms which swing weakly and cannot move forwards in seminal fluid after freezing and thawing, has no obvious influence on the physiological function of the sperms, has high freezing anabiosis rate, can be used for cryopreservation of less and weak sperms, testicle sperms and epididymis sperms, and contributes to the successful development of assisted reproductive technology.

The invention discloses a novel frozen semen diluent used for improving the freeze-thaw quality and fertilization rate of frozen bovine semen and a preparation method thereof. Every 100ml of the prepared frozen semen diluent contains 0.9-1.2g of sodium citrate, 3-5g of sucrose, 18-22ml of yelk, 4-7ml of glycerin, 6-9mg of vitamin E, 500-700mg of vitamin C, 35mg of spectinomycin and 80 thousand units of gentamicin. The invention adds VE and VC into the semen diluent for the first time, so as to increase the motility rate and acrosome integrity of the frozen sperm as well as the activity of antioxidant enzyme in seminal plasma, thus improving the quality and fertilization rate of the freeze-thaw sperm.

A Bacillus strain characterized by (i): sensitivity for ampicillin, vancomycin, gentamicin, kanamycin, streptomycin, erythromycin, clindamycin, tetracycline and chloramphenicol; (ii) antimicrobial activity against E. coli and Clostridium perfringens; and (iii) a sporulation percentage of at least 80 when measured after 2 days of incubation. The invention further relates to a method for selecting such strains. Many of the identified strains according to the invention are of the species Bacillus amyloliquefaciens. Some of the Bacillus amyloliquefaciens were further identified as Bacillus amyloliquefaciens subsp. amyloliquefaciens whereas others were identified as amyloliquefaciens subsp. plantarum. A Bacillus strain of the invention may be used as a feed additive to animal feed where it has a probiotic effect.

The invention relates to escherichia coli CGMCC NO.3192 for recombined engineering and a variant thereof. The strain is obtained by integrating gene segments subjected to the recombined engineering, i.e. a regulatory gene araC of the Arabinose ara operon from the escherichia coli, a promoter pBAD of the Arabinose ara operon from the escherichia coli, recombined enzyme genes red alpha, red alpha beta and gam from a gamma bacteriophage, a gene recA and gentamicin resistance gene (Gm) from the escherichia coli, into an endA gene area of a gene group of the escherichia coli DH10B, wherein red alpha, red alpha beta, gam and recA are driven by pBAD induced by arabinose. The recombined enzyme can catalyze homologous recombination among DNA short segments to finish the recombined engineering. The length of the DNA segment is about 50 base pairs. The invention has convenient operation of carrying out the recombined engineering by the strain and high recombined efficiency, thereby becoming the universal strain for the recombined engineering.

The invention discloses a preparation method of 1-N-ethyl gentamicin C1a sulfate. The method comprises the following steps of: carrying out a complexation reaction on gentamicin C1a and zinc acetate in a methanol solvent; then, dropping a mixed solution of acetic anhydride, triethylamine and tetrahydrofuran to carry out an acylation reaction and obtaining 3,2',6'-3-N-acetyl-gentamicin C1a by post-treatment; then, carrying out a silylation reaction with hexamethyl disilazane in a chloroform solvent; carrying out an N-alkylation reaction with acetaldehyde in a carrene solvent; then, carrying out a reduction reaction with potassium borohydride; hydrolyzing with an NaOH solution; obtaining 1-N-ethyl gentamicin C1a by post-treatment; adding the 1-N-ethyl gentamicin C1a to anhydrous ethanol or anhydrous methanol for stirring or dissolving; then, dropping concentrated sulfuric acid; and obtaining 1-N-ethyl gentamicin C1a sulfate by post-treatment. The method of the invention has higher yield.

The invention discloses a preparation method and an application of a sensor for simultaneously detecting four aminoglycoside antibiotics and belongs to the technical field of novel function materials and biosensor analysis. According to the invention, a reference electrode is printed by the use of a silver paste and four working electrodes and a counter electrode are printed by the use of a conductive carbon paste on a rectangular substrate surface coated with a layer of a polyester film. Thus, a four-channel screen-printed electrode is prepared. The preparation method is characterized in that antibodies of four aminoglycoside antibiotics including streptomycin, gentamycin, kanamycins and spectinomycin are respectively fixed on surfaces of the four working electrodes by the use of a Pt nanoparticle-sulfated graphene-carboxymethyl chitosan conductive composite nano-material; and after the detection liquids are dripped, the four working electrodes are connected with a multichannel electrochemical workstation detection circuit so as to realize simultaneous detection of the four aminoglycoside antibiotics. The sensor provided by the invention is simple to prepare and convenient to process, requires low cost, and is convenient to carry. The detection method is simple and rapid and has high sensitivity and good specificity.

The invention discloses a fresh amnion preservation fluid, a fresh amnion preservation method and application thereof. The fresh amnion preservation fluid is made by adding water for injection to 9.0-12.0g of DMEM culture medium, 10.0-25.0g of chondroitin sulfate, 0.5-1.0g of sodium hyaluronate, 4.0-5.0g of HEPES, 10.0-15.0g of dextran, 1.0-6.0ml of gentamycin, 24.0-30.0mg of dexamethasone, 10.0-15.0ml of non-essential amino acid, 0.50-0.95g of glutathione, 0.0-50.0ml of fetal calf serum to 1000ml. The fresh amnion preservation method comprises the following steps: separating the amnion by a blunt, rinsing the amnion clean, and then processing the amnion by an antibiotic; cutting and packaging the amnion into a container with the amnion preservation fluid; storing the amnion preservation fluid in a refrigerator with the temperature of 4 DEG C, and rinsing hands clean with aseptic phosphate buffer before use. The fresh amnion preservation fluid has the following advantages: 1. the amnion preservation fluid is only stored in the refrigerator with the temperature of 4 DEG C; 2. the shelf life is prolonged to at least 20-30 days; 3. the amnion preservation fluid can be in batch processing and centralized storage, and 4. the amnion preservation fluid can be applied to various amnion transplantation repairs, for example (1) ocular surface reconstruction as a conjunctival substitute, (2) amnion transplantation for treating symblepharon, and (3) treatment of corneal ulcer caused by various reasons and the like.

The invention discloses a multi-residue colloidal-gold rapid detection kit and a detection method and application thereof, belonging to the field of immunology. The kit provided by the invention mainly comprises a multi-residue colloidal-gold rapid detection card; the detection card comprises a detector bar and a plastic card shell, and the detector bar is composed of a sample pad, a colloidal-gold antibody binding pad, a nitrocellulose membrane and a water absorbing pad. The colloidal-gold antibody binding pad comprises a colloidal gold labeled gentamycin monoclonal antibody, a colloidal gold labeled kanamycin monoclonal antibody and a colloidal gold labeled fluoroquinolone monoclonal antibody. The central part of the nitrocellulose membrane is coated with a detection line and a control line, wherein the detection line is arranged to be a protein conjugate and the control line is a goat anti-mouse IgG monoclonal antibody. The rapid detection kit provided by the invention can realize simultaneous detection of a plurality of residues only through simple pre-treatment, the whole operation process is simple, the kit is convenient to carry, result determination is rapid and accurate, and the kit is applicable to on-site monitoring and to qualitative screening of considerable samples.

The invention relates to a continuous separation and purification technology of gentamycin C1a. Efficient separation of a gentamycin C1a recovered liquid is realized through using a continuous chromatographic separation technology. The technology comprises the following steps: allowing recovered gentamycin C1a to enter a continuous chromatographic system, adsorbing, washing out impurities, eluting, collecting the obtained eluate, regenerating a chromatographic column, and concentrating the collected eluate to obtain gentamycin C1a. The gentamycin C1a separated in through the technology has the advantages of high yield and high purity, so the technology has the advantages of low cost, environmental protection and suitableness for industrial production.

The present invention provides a clear depot comprising at least one hydrophilic water-soluble pharmaceutically active agent selected from the group consisting of vancomycin, gentamicin, a pharmaceutically acceptable salt thereof and a mixture thereof, water, a phospholipid, an oil, optionally a pH adjusting agent, and a viscosity modifying agent selected from the group consisting of ethanol, isopropanol, and a mixture thereof, wherein the water present in the depot is no more than about 4 wt % relative to the total weight of the depot and the depot has a pH of between about 3 and about 6, method of making and administering same.

The invention relates to an application of Sargassum polysaccharide in medicine preparation used for treating kidney injury, the Sargassum polysaccharide is an active extract total polysaccharide in Sargassum graminifolium, the invention specifically relates to an application of Sargassum polysaccharide in kidney protection, and an application of Sargassum polysaccharide which can be mixed or individually used as antidotes in the pharmacy fields. The results show that the Sargassum graminifolium polysaccharide has certain protection effect on rat kidney, and can resist the kidney injury caused by medicines and chemicals such as gentamycin, cisplatin and high oxalic acid and the like.

The invention relates to preservation of human local living parts and in particular relates to a culture medium for keeping primary airway epithelial cells in the physiological state in vivo during in vitro culture. The invention is characterized in that the culture medium contains the following components (per liter), 12 g of DMEM / F12 medium, 30 mg of penicillin G sodium salt, 50 mg of streptomycin, 10 mg of insulin, 5 mg of transferring, 25 Mug of epidermal growth factors, 100 Mug of cholera toxin, 108.741 Mug of hydrocortisone, 1 g of bovine pituitary extract, 3 g of bovine serum albumin, 1.5*10<-2> Mug of retinoic acid, 50 mg of gentamycin, 0.5 mg of amphotericin B, 50 mL of fetal calf serum and the balance of water. The primary airway epithelial cells cultured by the culture medium have the ciliary beating function and can maintain the physiological state in vivo.

The invention discloses a cryopreservation solution and cryopreservation resuscitation method for liver primary cells and relates to the field of biotechnologies. The cryopreservation solution is prepared according to the following formula, including 1,000g of dH2O, 15.6g of DMEM / F12 culture medium powder, 1.2g of sodium bicarbonate, 300ng of dexamethasone, 10 micrograms of liver primary cell growth factor, 25mg of insulin, 0.3g of glutamine, 25mg of gentamycin, 50mg of penicillin, 25mg of clarithromycin, 0.1g of DMSO, 10g of PEG6000, 1g of polyvinyl pyrrolidone and 1g of 1,3-propylene glycol. According to the cryopreservation solution and cryopreservation resuscitation method for the liver primary cells, the problem that the liver primary cells are relatively low in survival rate after cryopreservation resuscitation and cannot adhere to walls for drug metabolism study is solved, and the developed new cell cryopreservation solution formula and the corresponding resuscitation method can enable the cryopreservation resuscitation survival rate of the cells to be over 80%.

The invention discloses a preparation method of gentamicin sulphate. The use levels of a spore slant culture medium, a seed culture medium and a fermenting culture medium used in the production process are improved. The method provided by the invention has the beneficial effects that impurities in a gentamicin sulphate product can be remarkably reduced so as to meet the standard of Chinese pharmacopoeia, and the production process is simple and easy to operate. Total impurities of the gentamicin sulphate product produced before improvement is greater than 10%, and sisomicin is greater than 5%, while the total impurities of the gentamicin sulphate product produced is less than 5%, sisomicin is less than 2%, and kanamycinum (micronomicin) is less than 3%.

The invention relates to the field of medicines, and particularly relates to an operation irrigating solution and a preparation method thereof. The irrigating solution comprises sodium hyaluronate and a solvent. The irrigating solution is characterized in that the solvent is normal saline, injection water, a ringer solution irrigating solution, a glucose irrigating solution, a mannitol irrigating solution, a chlorhexidine irrigating solution, a gentamycin normal saline irrigating solution, a povidone iodine irrigating solution, a bromogeramine irrigating solution, a glycine irrigating solution, a metronidazole irrigating solution or a physiological balanced solution; and the concentration of the sodium hyaluronate in the operation irrigating solution is 0.1-10g / L. The operation irrigating solution has the effects of operation irrigation, has functions of preventing adhesion and promoting wound healing after operation, and has the advantages of no toxic or side effect, good stability and excellent effect.

The invention relates to a Pediococcus acidilactici strain which is named Pediococcus acidilactici AAF 3-3. The Pediococcus acidilactici AAF 3-3 is classified as Pediococcus acidilactici, and is preserved under the preservation number of CGMCC No. 17856 since the preservation date of May 27, 2019 in the preservation unit of China General Microbiological Culture Collection Center (No.3, Court 1, West Beichen Road, Chaoyang District, Beijing). The Pediococcus acidilactici AAF 3-3 disclosed by the invention has high tolerance to main stress pressures (acetic acid, lactic acid, high temperature and ethanol) and an extreme pH value during solid-state fermentation of edible vinegar, and has an L-lactic acid production function; and moreover, the Pediococcus acidilactici AAF 3-3 has significant inhibitory effects on escherichia coli, micrococcus luteus and staphylococcus aureus as well as resistance to gentamicin, chloramphenicol, erythromycin, ampicillin and kanamycin. Thus, the Pediococcusacidilactici AAF 3-3 has application potential in the field of foods.

The invention discloses an antibacterial modified graphene oxide and a preparation method thereof. The preparation method comprises following steps: weighing a certain amount of graphene oxide, addinga certain amount of water, carrying out dispersing under the assistance of ultrasonic waves for one hour to obtain evenly dispersed graphene oxide water dispersion; weighing a certain amount of polyhexamethylene guanidine hydrochloride, gentamycin, penicillin, kanamycin, and etimicin, adding the components mentioned above into the graphene oxide water dispersion in sequence, stirring the grapheneoxide water dispersion for 1 hour under the assistance of ultrasonic waves, adjusting the pH, carrying out reactions for 48 hours at a temperature of 60 DEG C under mechanical stirring, cooling the reaction product to the room temperature, performing centrifugal washing for three times, placing obtained centrifugal product in a vacuum baking oven with a temperature of 40 DEG C, and drying the centrifugal product in vacuum for 24 hours to obtain the antibacterial modified graphene oxide. The prepared antibacterial modified graphene oxide has the advantages that the equipment requirements are low, the technology is simple, after modification, the antibacterial performance of graphene oxide is largely improved, and the modified graphene oxide has a very good antibacterial performance and biocompatibility and can be used in the fields of biomedicine and antibiosis.

The invention discloses a spectinomycin, streptomycin, gentamycin and neomycin detection test strip and a method thereof. The test strip comprises micro-porous strips, micro-porous reagents, a test paper and micro-porous plugs, the micro-porous reagents are lyophilized colloidal gold labeled spectinomycin, streptomycin, gentamycin and neomycin specific antibodies respectively, the test paper is formed by sequentially connecting a sample absorption pad, a reaction film, a water absorption pad, a protection film and a substrate, the reaction film comprises four detection lines and a quality control line, the four detection lines are coated with a spectinomycin-carrier protein conjugate, a streptomycin-carrier protein conjugate, a gentamycin-carrier protein conjugate and a neomycin-carrier protein conjugate respectively, and the quality control line is coated with an antiantibody. The method for detecting the spectinomycin, streptomycin, gentamycin and neomycin by using the test strip has the advantages of simplicity, rapidness, perceptual intuition, accuracy, portability, wide application range, low cost, and easy popularized use.

The invention provides a preparation method and application of a product obtained by coupling gentamicin with carrier protein, as well as a gentamicin antibody. Animals are immunized with gentamicin artificial antigen coupled in the invention so as to prepare the antibody which can be used for detecting gentamicin in foods. The preparation method comprises the following steps: immune BALB / C mouse spleen cells and SP2 / 0 mouse myeloma cells are fused; the gentamicin coupled with the carrier protein is used as coating antigen to screen positive hybridoma; hybridoma capable of stably transferring culture and secreting anti-gentamicin antibodies through cell clones is obtained; and an ascites monoclonal antibody is prepared. The prepared monoclonal antibody is utilized to establish a direct competitive ELISA method having high specificity, sensitivity and accuracy to the gentamicin, as well as an immune colloidal gold test strip. The preparation method for the product obtained by coupling gentamicin with carrier protein, as well as the gentamicin antibody can serve the rapid detection of gentamicin residue in foods.

The invention discloses a gentamycin immunoassay reagent as well as a preparation method and a detection method thereof. The gentamycin immunoassay reagent comprises enzyme labeled gentamycin and an indicator reagent used for detecting a gentamycin antibody-enzyme labeled gentamycin compound; and the enzyme labeled gentamycin formed by coupling the gentamycin to glucose dehydrogenase. The gentamycin immunoassay reagent provided by the invention can accurately and rapidly determine the content of the gentamycin in a sample such as human blood. Compared with the existing detection reagent on themarket, the gentamycin immunoassay reagent has the advantages of convenience, rapidness, high sensitivity, strong specificity, accuracy in quantification and the like and is beneficial to clinical popularization and use.

A method of treating prostate cancer in a living mammal comprising the topical administration of a composition containing a therapeutically effective concentration of collagenase. In one embodiment, a method of treating prostate cancer in a living mammal includes topical administration of a protein containing a therapeutically effective concentration of collagenase and at least one glycosidase, protease, nuclease, lipase, esterase, plasminogen activator, streptokinase and their mixtures. Preferably a glycosidase enzyme such as hyaluronidase is administered. Compositions useful in methods of treating prostate cancer also include or are administered together with calcium ions, nonionic surfactants such as Triton RX-100, and antibiotics such as gentamicin. Other methods of treating prostate cancer in living mammals include activation of prostate-specific-antigen (PSA) in vivo, such as by local administration of calcium ions.

The present invention relates to a marker gene for screening a drug inducing toxicity in human and a screening method using the same. More precisely, the invention relates to a microarray on which marker genes up-or down-regulated specifically by 16 drugs inducing pulmonary toxicity, teratogenicity, nephrotoxicity, cardiotoxicity or mutation (Methotrexate, Nitrofurantoin, Amiodarone, Carbamazepine, Valproic acid, Thalidomide, Cisplatin, Gentamycin, Amphotericine, Furylfuramide, N-nitroso-N-methylurea, methylmethanesulfonate, 4-nitroquinoline-N-oxide, 2-nitrofluorene, Doxorubicin and Daunorubicin) are integrated, a kit comprising the said microarray, and a screening method of a drug inducing toxicity in human using the same. The DNA microarray containing the marker gene of the present invention facilitates the construction of Toxtarget Array for screening a drug inducing toxicity in human using drug-specific genes, suggesting that this chip can be effectively used for monitoring drugs or chemicals carrying toxicity to human or determining risks thereof and also it can be used as a tool for examining mechanisms of toxicity / side effects caused by the drugs.

The invention discloses long-term preservation liquid for corneal tissue and a preparation method thereof. The long-term preservation liquid is prepared from the following components: 15 to 25g / L of sodium chondroitin sulfate, 0.5 to 4g / L of sodium hyaluronate, 0.5 to 3 g / L of dextran 40, 15 to 30 g / L of glycerin, 0.001 to 0.3 g / L of sodium pyruvate, 0.002 to 2 g / L of vitamin C, 0.05 to 0.7 g / L ofgentamicin sulfate, 1.4 to 2.2 g / L of sodium bicarbonate, 5.0 to 6.5 g / L of 4-hydroxyethylpiperazine ethanesulfonic acid, and 1 L of water for injection. A combination system of sodium chondroitin sulfate, sodium hyaluronate and dextran is adopted to obtain the preservation liquid which can be used for long-term preservation of corneal tissue, thereby being particularly advantageous for maintaining an original collagen fiber structure and transparency of a corneal lens for a long term; after the cornea tissue is preserved by the long-term preservation liquid, the cornea tissue can be used directly after rewarming, the operation is simple, and rehydration is not needed.

The invention relates to the technical field of frozen cattle semen production and provides a diluent for frozen cattle semens. The diluent comprises a diluent A and a diluent B, wherein per 100ml of the diluent A comprises the following raw materials: 2.5-3.4g of trisodium citrate or sodium citrate, 0.1-0.2g of gentamycin, 0.03-0.1g of Ciprofloxacin, 50-100 million thousand units of ampicillin sodium, 50-100 million thousand units of streptomycin, 1-2g of levulose, 10-15ml of yolk and the balance of distilled water; and the diluent B is prepared by adding 12-16ml of glycerol or glycol into the 84-88 percent by volume of the diluent A used as a base solution. The preparation method mainly comprises the following steps of: weighting various raw materials and the distilled water for later use; preparing the base solution; preparing the diluent A; and preparing the diluent B. The diluent can be used for effectivlely reducing the bacterium content of the frozen cattle semens, is good in antibiotic effect, and can be used for greatly improving the frozen cattle sement production efficiency and the frozen cattle semen quality; and the preparation method is simple to operate and is economical and practical.

The invention discloses a quantum-dot immunofluorescence kit for simultaneously detecting gentamycin and quinolone medicines, and at the same time, the invention also discloses a preparation method of ciprofloxacin hapten, corresponding artificial antigen and a quantum dot-labelled monoclonal antibody. The kit takes a conjugate of gentamycin semiantigen and carrier protein as well as the conjugate of the ciprofloxacin semiantigen and carrier protein as a primordial covering, and takes a mixture of a quantum dot-labelled gentamycin specific antibody and a ciprofloxacin specific antibody as a detection antibody. The preparation method is simple and feasible, has the advantages of low cost and high semiantigen yield, and the quantum-dot immunofluorescence kit has the advantages of simpleness, rapidity, large sample processing amount, high sensitivity, and strong specificity. The quantum-dot immunofluorescence kit can be used for self-control of quality and food safety supervision of a food production enterprise, and routine screening of a detection mechanism, and can be used as an auxiliary means for animal pharmacological and toxicologic researches in scientific research.

The invention discloses an antibiotic lysobacter spp gene knockout system as well as a construction method and an application thereof, and belongs to the technical field of microbiological genetic engineering. A gene knockout mutant is prepared by the following steps: constructing a recombinant vector for gene knockout by virtue of a suicide plasmid; transferring the recombinant vector into competent cells; conducting primary recon screening and secondary recon screening on a gentamicin containing resistance medium and a sucrose containing medium; and conducting mutant screening by virtue of PCR (polymerase chain reaction). With the application of the knockout system, two genes in antibiotic lysobacter spp OH13 can be successfully knocked out, proving that the system is applicable to gene function researches of the antibiotic lysobacter spp; therefore, a foundation is laid for researches on small-molecule metabolite biosynthesis, genetic regulation and action mechanism.

Bone cement compositions and methods of making two-part bone cements. The methods comprise transferring premixed powder and a liquid component into a receptacle, and mixing the powder component and the liquid component to form a cement composition. The premixed powder component comprises an acrylic polymer and a radical initiator. The bone cement is loaded with from about 5% to about 6% gentamicin by weight of the powder component, and from about 4% to about 5% vancomycin by weight of the powder component. The methods include filling a mold cavity with the bone cement composition to form a temporary spacer implant. The bone cement compositions provide a viscosity profile sufficient for the bone cement composition to flow within the mold cavity for an elapsed working time period of greater than about 6 minutes from the start of mixing at approximately 23° C.

The invention discloses streptomyces albulus (GS-114) and a method of the same to prepare epsilon-polylysine, and belongs to the field of microbial fermentation engineering. The provided streptomycesalbulus (GS-114) has resistance to streptomycin, gentamicin and rifamycin with 3 [mu]g / mL, 1 [mu]g / mL and 0.1 [mu]g / mL or higher concentrations, and can efficiently synthesize epsilon-PL in quantity,the yield with 192 h of fermentation can reach 56.3 g / L, and production efficiency can reach up to 7.03 g / L / d. The streptomyces albulus (GS-114) also has good passage stability and has strong potential for the industrial production of the epsilon-PL.

A fungus (Aspergillus terreus FZC3) with the preservation number of CGMCC No.12072 can be applied to grade gentamycin, and the fungus is inoculated to a system containing gentamycin and is cultured to realize degradation of gentamycin. The fungus is cultured to remove gentamycin in environment, and can be used to process pharmaceutical wastes in order to reduce harms of gentamycin to environment, humans and animals.