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Diluent for frozen cattle semens and preparation method of diluent

A diluent and frozen semen technology, which is applied in the field of bovine semen diluent and its preparation, can solve the problems of low viability after freezing and unsatisfactory antibacterial effect, achieve high vigor after freezing, good antibacterial effect, and improve the quality of semen Effect

Inactive Publication Date: 2013-03-06
XINJIANG TIANSHAN ANIMAL HUSBANDRY BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the defects that the number of bacteria contained in bovine semen after freezing is large, the activity after freezing is low, and the antibacterial effect is not ideal, the purpose of the present invention is to provide a method that can effectively reduce the number of bacteria contained in bovine semen after freezing, has good antibacterial effect, and can Another object of the invention is to provide a simple, economical and practical method for preparing the bovine semen frozen diluent

Method used

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  • Diluent for frozen cattle semens and preparation method of diluent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A kind of frozen semen dilution of bovine semen, said diluent of frozen semen of bovine comprises A liquid and B liquid, and wherein A liquid is that the content of each raw material in every 100ml dilution mainly comprises:

[0029] Trisodium citrate 2.9g, gentamicin 0.16g, ciprofloxacin 0.04g, ampicillin sodium 80,000 units, streptomycin 50,000 units, fructose 1.1g, egg yolk 12ml, and distilled water as the rest.

[0030] Among them, liquid B is prepared from liquid A with a volume ratio of 85% as a base liquid, and 15ml of glycerin is added to it, that is, the main raw material content includes:

[0031] Trisodium citrate or sodium citrate 2.465g, gentamicin 0.136g, ciprofloxacin 0.34g, ampicillin sodium 68,000 units, streptomycin 42,500 units, fructose 0.935g, egg yolk 10.2ml, glycerin 15ml, the balance is distilled water.

[0032] The preparation method of described bovine semen diluent is characterized in that comprising the following steps:

[0033] (1) Weigh 2...

Embodiment 2

[0039] Example 2: The difference from Example 1 is:

[0040]A kind of frozen semen dilution of bovine semen, said diluent of frozen semen of bovine comprises A liquid and B liquid, and wherein A liquid is that the content of each raw material in every 100ml dilution mainly comprises:

[0041] Sodium citrate 2.9g, gentamicin 0.16g, ciprofloxacin 0.04g, ampicillin sodium 80,000 units, streptomycin 50,000 units, fructose 1.1g, egg yolk 12ml, and distilled water as the rest.

[0042] Among them, liquid B is obtained from liquid A with a volume ratio of 85% as a base liquid, and is prepared by adding 15ml of ethylene glycol to it, that is, the main raw material content includes:

[0043] Trisodium citrate or sodium citrate 2.465g, gentamicin 0.136g, ciprofloxacin 0.34g, ampicillin sodium 68,000 units, streptomycin 42,500 units, fructose 0.935g, egg yolk 10.2ml, B Diol 15ml, the balance is distilled water.

[0044] The preparation method of described bovine semen diluent is char...

Embodiment 3

[0050] Example 3 : The difference from Example 1 is:

[0051] A kind of frozen semen dilution of bovine semen, said diluent of frozen semen of bovine comprises A liquid and B liquid, and wherein A liquid is that the content of each raw material in every 100ml dilution mainly comprises:

[0052] Trisodium citrate 2.5g, gentamicin 0.1g, ciprofloxacin 0.03g, ampicillin sodium and streptomycin each 50,000 units, fructose 1g, egg yolk 10ml, and distilled water as the rest.

[0053] Wherein, liquid B is obtained from liquid A with a volume ratio of 84% as a base liquid, and is prepared by adding 16ml of glycerin therein.

[0054] The preparation method of described bovine semen diluent is characterized in that comprising the following steps:

[0055] (1) Weigh 2.5g of trisodium citrate, 0.1g of gentamicin, 0.03g of ciprofloxacin, 50,000 units each of ampicillin sodium and streptomycin, 1g of fructose, 10ml of egg yolk, and 16ml of glycerin and distilled water for use;

[0056] ...

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Abstract

The invention relates to the technical field of frozen cattle semen production and provides a diluent for frozen cattle semens. The diluent comprises a diluent A and a diluent B, wherein per 100ml of the diluent A comprises the following raw materials: 2.5-3.4g of trisodium citrate or sodium citrate, 0.1-0.2g of gentamycin, 0.03-0.1g of Ciprofloxacin, 50-100 million thousand units of ampicillin sodium, 50-100 million thousand units of streptomycin, 1-2g of levulose, 10-15ml of yolk and the balance of distilled water; and the diluent B is prepared by adding 12-16ml of glycerol or glycol into the 84-88 percent by volume of the diluent A used as a base solution. The preparation method mainly comprises the following steps of: weighting various raw materials and the distilled water for later use; preparing the base solution; preparing the diluent A; and preparing the diluent B. The diluent can be used for effectivlely reducing the bacterium content of the frozen cattle semens, is good in antibiotic effect, and can be used for greatly improving the frozen cattle sement production efficiency and the frozen cattle semen quality; and the preparation method is simple to operate and is economical and practical.

Description

technical field [0001] The invention relates to the technical field of bovine frozen semen production, in particular to a bovine frozen semen diluent and a preparation method thereof, belonging to the field of biotechnology. Background technique [0002] The combination of bovine semen cryopreservation and artificial insemination technology plays an extremely important role in modern cattle production. The use of frozen semen for artificial insemination can not be limited by time, region, cattle breed and individual, and can greatly reduce the number of bulls. , reduce production costs, improve the utilization rate of good breeding bulls, and bring huge economic benefits to the cattle industry. At the same time, the development of bovine semen freezing technology and the use of bovine frozen semen are of great significance in the introduction of bovine breeds, hybrid improvement, improvement of reproductive performance, disease control and protection of bovine genetic divers...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 朱兵山丁娟
Owner XINJIANG TIANSHAN ANIMAL HUSBANDRY BIO ENG
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