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Preparation method of gentamicin sulphate

A technology of gentamicin sulfate and gentamicin sulfate, which is applied in the field of preparation of gentamicin sulfate, can solve the problems of difficult compliance and high impurities in products, and achieve the effect of simple and easy operation of the production process and the reduction of impurities

Active Publication Date: 2013-06-19
福安药业集团烟台只楚药业有限公司
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

However, using the existing gentamicin production formula for fermentation and production, the product has high impurities, which is difficult to meet the requirements of the 2010 edition of the "Chinese Pharmacopoeia"

Method used

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Examples

Experimental program
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Embodiment 1

[0051] The preparation method of gentamicin sulfate includes the following steps:

[0052] 1) Take the gentamicin sand soil spores and place them on the spore slant medium for spore culture, the culture temperature is 36°C, the culture time is 10 days, and the gentamicin spores after the slant culture are obtained;

[0053] The preparation method of gentamicin sand soil spores is:

[0054] a. The formula of sand soil spore culture medium is calculated by weight percentage: soluble starch 1.0%, sodium chloride (NaCl) 0.05%, potassium nitrate (KNO 3 ) 0.1%, calcium carbonate (CaCO 3 )0.1%, dipotassium hydrogen phosphate (K 2 HPO 4 )0.02%, bran 1.8%, magnesium sulfate (MgSO 4 ) 0.02%, agar 2.0%, the balance is water (in this example, soluble starch 1.0g, sodium chloride (NaCl) 0.05g, potassium nitrate (KNO 3 ) 0.1g, calcium carbonate (CaCO 3 )0.1g, dipotassium hydrogen phosphate (K 2 HPO 4 )0.02g, bran 1.8g, magnesium sulfate (MgSO 4 ) 0.02g, agar 2.0g, water 94.91g); the prepared sandy ...

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Abstract

The invention discloses a preparation method of gentamicin sulphate. The use levels of a spore slant culture medium, a seed culture medium and a fermenting culture medium used in the production process are improved. The method provided by the invention has the beneficial effects that impurities in a gentamicin sulphate product can be remarkably reduced so as to meet the standard of Chinese pharmacopoeia, and the production process is simple and easy to operate. Total impurities of the gentamicin sulphate product produced before improvement is greater than 10%, and sisomicin is greater than 5%, while the total impurities of the gentamicin sulphate product produced is less than 5%, sisomicin is less than 2%, and kanamycinum (micronomicin) is less than 3%.

Description

Technical field [0001] The invention relates to the field of antibiotic fermentation, in particular to a preparation method of gentamicin sulfate. Background technique [0002] Gentamicin sulfate is an aminoglycoside antibiotic. It has a good antibacterial effect on various gram-negative bacteria and gram-positive bacteria, and against various enterobacteriaceae such as Escherichia coli, Klebsiella, and Proteus Genus, Salmonella, Shigella, Enterobacter, Serratia, and Pseudomonas aeruginosa have good antibacterial effects. Neisseria and Haemophilus influenzae are moderately sensitive to this product. It also has a certain effect on Brucella, Yersinia pestis, Acinetobacter and Campylobacter fetus. It has a good antibacterial effect on about 80% of methicillin-sensitive strains in Staphylococcus (including Staphylococcus aureus and coagulase-negative Staphylococcus), but most of the methicillin-resistant strains are resistant to this product. The effect on Streptococcus and Strept...

Claims

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Application Information

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IPC IPC(8): C12P19/48
Inventor 王玉红葛祥斌
Owner 福安药业集团烟台只楚药业有限公司
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