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Gentamicin and carrier protein couplet product, method for producing gentamicin antibody and uses thereof

A gentamicin and carrier protein technology, which is applied in the field of gentamicin and carrier protein coupling products and the preparation of gentamicin antibodies, can solve problems such as high price, and achieve accurate and reliable results, high sensitivity, and sensitive cost. Effect

Inactive Publication Date: 2009-05-13
ZHEJIANG UNIV
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  • Description
  • Claims
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Problems solved by technology

Some foreign manufacturers have developed ELISA detection kits for some drug residues, but they are expensive

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  • Gentamicin and carrier protein couplet product, method for producing gentamicin antibody and uses thereof

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Embodiment Construction

[0027] The invention provides the preparation method and application of the coupling product of gentamicin and carrier protein and gentamicin antibody. Using the prepared immunogen to prepare monoclonal antibodies and polyclonal antibodies with strong specificity, high sensitivity, good stability, and mass production of gentamicin, and using these antibodies to establish a highly specific method for the detection of gentamicin residues in food Rapid diagnostic methods with high sensitivity and correctness—competitive ELISA and immunochromatographic test strips can be effectively used in the detection of gentamicin residues in animal products and serve the food safety of our country.

[0028] 1. the step of a preparation method of gentamicin and bovine serum albumin (BSA) coupling product:

[0029] a) 20 mg of gentamicin sulfate and 10 mg of BSA were dissolved in 1 ml of 0.01M pH7.2 phosphate buffer;

[0030] b) Add 1 ml of an ultrapure aqueous solution containing 300 mg of ca...

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Abstract

The invention provides a preparation method and application of a product obtained by coupling gentamicin with carrier protein, as well as a gentamicin antibody. Animals are immunized with gentamicin artificial antigen coupled in the invention so as to prepare the antibody which can be used for detecting gentamicin in foods. The preparation method comprises the following steps: immune BALB / C mouse spleen cells and SP2 / 0 mouse myeloma cells are fused; the gentamicin coupled with the carrier protein is used as coating antigen to screen positive hybridoma; hybridoma capable of stably transferring culture and secreting anti-gentamicin antibodies through cell clones is obtained; and an ascites monoclonal antibody is prepared. The prepared monoclonal antibody is utilized to establish a direct competitive ELISA method having high specificity, sensitivity and accuracy to the gentamicin, as well as an immune colloidal gold test strip. The preparation method for the product obtained by coupling gentamicin with carrier protein, as well as the gentamicin antibody can serve the rapid detection of gentamicin residue in foods.

Description

technical field [0001] The invention belongs to the technical field of immunochemistry and biology, and in particular relates to a preparation method and application of a coupling product of gentamicin and carrier protein and gentamicin antibody. Background technique [0002] The control of drug residues requires not only perfect inspection methods and standards, but also a sound monitoring system. At present, there are mainly instrumental analysis methods (including HPLC and GC-MS) and microbiological methods (mainly used for the detection of antibiotic residues) for the detection of antibiotic drug residues in the world. The instrumental analysis method requires not only high precision of instruments and equipment, but also good analytical operation skills of technicians. Its expensive price, slow software update speed and cumbersome operation limit its application in general laboratories. . However, since the detection sensitivity can reach ng level (ppb level), it is s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/795C07K14/765C07K14/77C07K16/00C07K16/44C07K16/06C12N9/00G01N33/53G01N33/577
Inventor 吴建祥张少恩
Owner ZHEJIANG UNIV
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