The invention discloses a preparation method of
fucoidan sulfuric esterwith high purity and high-
sulfate radical content, comprising the following steps: (1) adding water in dry
kelp for soaking and rinsing to remove
sediment and then
grinding; (2) adding the
kelp in 95 percent
ethanol for stirring, extracting and separating to obtain
kelp residue A; (3) adding water in the kelp residue A, adjusting the pH value to 6-8, and stirring, extracting and separating the mixture to obtain kelp residue B and supernatant; (4) adding super
calcium chloride in the supernatant, standing and filtering to obtain filtrate; (5) condensing the filtrate to concentrated solution; and (6) directly spraying and
drying the condensed solution, or depositing the
fucoidan with 40-70 percent
ethanol and
drying and crushing the dried
fucoidan, and finally obtaining the fucoidan
sulfuric ester with high purity and high-
sulfate radical content, wherein the content of
fucose is 40-70 percent and the content of
sulfate radical is 30-50 percent. Compared with the prior art, the invention is free from using acid base for treatment and adjusting the pH value, has simple method, less consumption of
organic solvent, few steps and low cost, and is suitable for industrial production.