Lipase determination kit and preparation method thereof

Abstract

The invention discloses a lipase determination kit and a preparation method thereof. An R1 reagent comprises a buffer solution, sodium deoxycholate, colipase, metal salt, a protective agent, a surfactant and a preservative; an R2 reagent comprises Ltartaric acid, calculus bovis deoxycholic acid sodium salt, 1, 2-o-dilauroyl racemic glycerol 3valeric acid (6methyl resorufin) ester, a cosolvent, a stabilizer and a preservative, and the cosolvent is composed of ethyl acetate, dilauroyl glycerol sulfate and a Triton surfactant. According to the invention, through optimization, ethyl acetate, dilauryl glyceryl sulfate and Triton surfactant are jointly used as a cosolvent to play a synergistic effect of the three components, so that the solubility of a substrate is greatly improved, and further,mercaptoethanol and mannitol are used as composite stabilizers, therefore, the lipase determination kit which is good in stability, high in accuracy and precision and remarkably improved in linear range is obtained.

Description

technical field [0001] The invention belongs to the technical field of clinical biochemical detection, and in particular relates to a lipase assay kit and a preparation method thereof. Background technique [0002] Serum lipase (LPS) is mainly derived from the pancreas, and a small amount is derived from the stomach and small intestine. The determination of lipase activity is used to reflect the disorder of the pancreas. Increased human lipase is common in acute pancreatitis, pancreatic cancer, pancreatic duct obstruction due to stones, liver cirrhosis, intestinal obstruction, acute and chronic kidney disease, etc. LPS is a group of enzymes that catalyze the hydrolysis of long-chain fatty acid glycerides, which only hydrolyzes the α-position fatty acids of fatty acid glycerides. LPS has a certain specificity for long-chain fatty acid glycerides, and the maximum activity and specificity of LPS requires the participation of cholate and co-lipase. Chromogen substrate method a...

AI Technical Summary

Problems solved by technology

However, in the preparation process of the R2 reagent, there will be insufficient dissolution of the chromogen substrate 1,2-o-dilauryl-racem-glycerol-3-pentanoate-(6-methylresorufin) ester, which will affect The batch-to-batch difference and stability of the reagent, and as the storage time increases, there will be substrate precipitation in the R2 reagent, so that the measured value will decrease with the increase of the storage time; while the linearity of the lipase kit in the prior art The range is narrow, resulting in the need to dilute the sample before testing when detecting high values, thus increasing the workload of clinical testing

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