The invention discloses an extraction method of microalga
grease. The extraction method comprises the following steps of: (1) microalga
cell collection: adjusting the pH value of a microalga culture solution to 11-12 at the later period of microalga cultivation, and collecting microalga cells in a centrifugal way; (2) microalga
cell crushing: adding a C1-C6 short-chain
alcohol solvent into microalga ooze obtained in the step (1), shaking and mixing the microalgae to be uniform, and then, carrying out
cell breaking treatment on the microalgae; (3)
grease extraction: adding one or more of organic solvents such as n-
hexane and
petroleum ether into a microalga cell breaking solution obtained in the step (2), and extracting the
grease component in the cell breaking solution by using the organic solvents; and (4)
solvent recovery: after the extraction reaction is ended, cooling the
solvent to
room temperature, standing for 10-30min, respectively extracting an
organic solvent layer (
oil phase) and a
water layer (water phase), and recovering the organic solvents in the
oil phase in a
distillation way, and meanwhile, obtaining the microalga grease. The method has the advantages of simple operation process, short extraction time, low
energy consumption, high microalga grease yield and the like and is suitable for directly extracting grease from alga ooze.