Anti-tumor activating fuling scloerotium glucan sulfate and its preparation and use
A technology of dextran sulfate and antitumor activity, applied in the fields of polymer chemistry and molecular biology, can solve the problems of few reports on derivatization modification, achieve good application prospects, short production cycle, simple and easy production process row effect
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Embodiment 1
[0038] Fresh Poria sclerotia were collected from Jiuzihe Town, Luotian County, Hubei Province. The sclerotia were successively refluxed with ethyl acetate and acetone for 6 hours respectively for Soxhlet extraction to remove fat. Then soak in normal saline to extract and centrifuge; the residue after centrifugation is extracted with hot water at 120°C at 0.20Mpa and centrifuged; the residue after centrifugation is washed with 0.5M NaOH (containing 0.05% NaBH 4 ) at 4°C to obtain polysaccharides, which are white powdery solids. Gas chromatography, infrared spectroscopy and13 Its structure was detected by C NMR spectrum, etc., which proved that it was a high-purity linear (1→3)-β-D-glucan.
[0039] Dissolve 600 mg of the above Poria cocos sclerotium (1→3)-β-D-glucan in 50 mL of DMSO, stir at room temperature for 12 hours, then add 9 mL of pyridine dropwise at a rate of 0.8 mL / min, and continue stirring at room temperature 30 minutes. The reaction flask was placed in an ice ba...
Embodiment 2
[0041] Fresh Poria sclerotia were collected from Jiuzihe Town, Luotian County, Hubei Province. The sclerotia were refluxed with ethyl acetate and acetone for 5 hours respectively for Soxhlet extraction to remove fat. Then soak in normal saline to extract and centrifuge; the residue after centrifugation is extracted with hot water at 120°C at 0.20Mpa and centrifuged; the residue after centrifugation is washed with 1M NaOH (containing 0.1% NaBH 4 ) at 5° C. to obtain polysaccharides, which are white powdery solids. Gas chromatography, infrared spectroscopy and 13 Its structure was detected by C NMR spectrum, etc., which proved that it was a high-purity linear (1→3)-β-D-glucan.
[0042] Dissolve 600 mg of the above Poria cocos sclerotium (1→3)-β-D-glucan in 50 mL of DMSO, stir at room temperature for 16 hours, then add 9 mL of pyridine dropwise at a rate of 1.0 mL / min, and continue stirring at room temperature 25 minutes. The reaction flask was placed in an ice bath, and 3.7 ...
Embodiment 3
[0044] Fresh Poria sclerotia were collected from Jiuzihe Town, Luotian County, Hubei Province. The sclerotia were successively refluxed with ethyl acetate and acetone for 6 hours respectively for Soxhlet extraction to remove fat. Then soak in normal saline to extract and centrifuge; the residue after centrifugation is extracted with 105°C hot water at 0.12Mpa and centrifuged; the residue after centrifugation is washed with 0.5M NaOH (containing 0.05% NaBH 4 ) at 4°C to obtain polysaccharides, which are white powdery solids. Gas chromatography, infrared spectroscopy and 13 Its structure was detected by C NMR spectrum, etc., which proved that it was a high-purity linear (1→3)-β-D-glucan.
[0045] Dissolve 600 mg of the above Poria cocos sclerotium (1→3)-β-D-glucan in 50 mL of DMSO, stir at room temperature for 12 hours, then add 9 mL of pyridine dropwise at a rate of 1.0 mL / min, and continue stirring at room temperature 30 minutes. The reaction flask was placed in an ice bat...
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