The invention relates to a quantum dot doped gel and preparation and application thereof, belonging to the technical field of preparation and application of nanocomposites. The gel is a polymer in which one or more selected from the group consisting to CdTe, CdTe / CdS, CdZnTe, CdZnTe / CdS, ZnSe, ZnSe / ZnS, CdSe, CdSe / CdS, CdSe / ZnS, ZnCdSe, CdSe / CdS / ZnS and CdTe / CdS / ZnS are dispersed, and the polymer is one or more selected from the group consisting of agar, gelatin, chitosan, polyvinyl alcohol, polyethylene glycol, carragheenan, hyaluronic acid and polyacrylamide. The gel is prepared by adding 1 to 25 wt% of the polymer into 0.1 to 10 wt% of a quantum dot aqueous solution, carrying out stirring under the protection of gas at a temperature of 0 to 40 DEG C for swelling of the polymer, then dissolving the polymer at a temperature of 50 to 100 DEG C and allowing the gel to be formed at a temperature of 0 to 40 DEG C. The gel can be used as a display material in three-dimensional holographic display.