The invention discloses a method for preparing natural
hydroxytyrosol by taking olive leaves as a
raw material and adopting an
ultrasonic assisted in-situ extraction process, and belongs to the technical field of extraction and separation of
natural product effective components. The method comprises the following steps: (1) pretreating the
raw material; (2) performing ultrasonic extraction synchronous leaching on
hydroxytyrosol in the olive leaves by using a double-phase
system which consists of an
organic solvent A and an alkaline solution; (3) performing
activated carbon decoloration on an
organic solvent extraction phase, then performing compressed concentration, and removing impurities by adopting an
organic solvent B; and (4) performing thermal melting on an extract which is obtained by virtue of
impurity removal by using an organic
solvent C, filtering while heating, and then performing compressed concentration and
drying to obtain a high-quality
hydroxytyrosol product. According to the process disclosed by the invention, the
cavitation effect and
mechanical vibration effect produced by ultrasonic
waves and an in-situ extraction process are adopted, extraction and leaching can be synchronously performed, and natural hydroxytyrosol with high content can be prepared by using simple separation and purification processes; and the method disclosed by the invention can be used for shortening the
production cycle, is high in product yield, mild in separation and purification conditions, small in
toxicity and low in production cost, and can be suitable for industrial production.