The invention relates to an ultrahigh-sensitivity fluorescent probe for detecting
nitrogen monoxide. The ultrahigh-sensitivity fluorescent probe has the structural general formula in the original text. During preparation, firstly, 4-
bromine-1, 8-anhydride
naphthalene and o-nitro p-phenylenediamine with equal
molar weights are used as raw materials, dissolved in
ethylene glycol
monomethyl ether and heated for reaction to obtain light yellow
solid. Secondly, the light yellow
solid is dissolved in
anhydrous DMSO, primary amine is added into the
anhydrous DMSO, the
anhydrous DMSO is heated and refluxed, and reaction liquid is filtered in a sucking manner, depressurized and dried to obtain yellow
solid. The yellow solid is dissolved in mixed liquid of
tetrahydrofuran and
methanol, and a yellow probe is obtained under
catalysis of
hydrogen and catalysts. Probe molecules have
fine chemical and
optical stability, good
solubility and biological compatibility and high
nitric oxide selectivity, and are free of interference of other
active oxygen,
active nitrogen and the like.
Laser confocal imaging experiments indicate that the probe has fine cellular permeability, has no toxic and side effects
on cells and organisms, and can be used for detecting protogenetic
nitrogen monoxide in a high-sensitivity manner.