The invention discloses a thin-layer
chromatography identification method of carbon
traditional Chinese medicine formula granules. The method comprises the steps that 1, sample
powder is taken and dissolved through water,
hydrochloric acid is added, heating is conducted for
hydrolysis,
filtration is conducted, filtrate is extracted through
ethyl acetate or ethyl
ether, extract liquor is evaporated to
dryness, and residues are dissolved through methyl
alcohol to serve as a
test sample solution; 2, reference medicinal materials are taken, decocted through water and filtered, filtrate is concentrated, and then a reference medicinal material solution is prepared through the method for preparing a
test sample; 3, the
test sample solution and the reference medicinal material solution are sucked and dripped on a same
silica gel GF254 thin-layer plate, and developing is conducted by taking a mixture of methylbenzene, one or more of
ethyl formate or
ethyl acetate or
butyl acetate and
formic acid as developing solvents according to the volume ratio of 2-8 to 2-8 to 0.1-1; 4, the thin-layer plate is viewed under a 254 nm
ultraviolet lamp, and principal spots with the same color are showed on the positions, corresponding to the
chromatography of the reference medicinal materials, in the test sample
chromatography. The method is rapid, comprehensive and high in specificity, and the requirements of carbonized herbs and the carbonizing
drug characteristic of the formula granules thereof can be embodied.