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Thin-layer chromatography identification method of carbon traditional Chinese medicine formula granules

A technology of traditional Chinese medicine formula granules and thin-layer chromatography, which is applied in the field of TLC identification of carbon-based traditional Chinese medicine formula granules, can solve problems such as lack of quality control methods, and achieve the effects of comprehensive detection results, simple operation, and strong specificity

Inactive Publication Date: 2016-01-13
GUANGDONG YIFANG PHARMA
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0005] Charcoal traditional Chinese medicine formula granules are made from charcoal traditional Chinese medicine decoction pieces by decoction extraction, concentration, spray drying, granulation and other processes. Compared with the original decoction pieces, their properties have changed greatly, and there is a lack of corresponding quality control methods.

Method used

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  • Thin-layer chromatography identification method of carbon traditional Chinese medicine formula granules
  • Thin-layer chromatography identification method of carbon traditional Chinese medicine formula granules
  • Thin-layer chromatography identification method of carbon traditional Chinese medicine formula granules

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0055] Experimental example 1 TLC identification of madder charcoal formula granules

[0056] Take 0.5g of madder charcoal formula granules, put them in a Erlenmeyer flask, add 15ml of water for ultrasonic dissolution, add diethyl ether for extraction twice, 20ml each time, discard the ether layer, add 5ml of 15% hydrochloric acid to the water layer, and heat in a water bath at 98°C for 60 minutes. Allow to cool and filter; the filtrate is extracted twice with ethyl acetate, 20ml each time, the ethyl acetate solution is combined, evaporated to dryness, and the residue is dissolved in 1ml of methanol as the test solution. Take another 2.0 g of Rubia reference drug, add 80 ml of water, boil for 30 minutes, filter, concentrate the filtrate to 15 ml, add ether to extract twice, and prepare the rest as the test sample to make a reference drug solution. According to the thin-layer chromatography ("Chinese Pharmacopoeia" 2010 edition, an appendix VIB) test, absorb 5 μl each of the ...

experiment example 2

[0057] TLC Identification of Experimental Example 2 Maogen Charcoal Formula Granules

[0058] Take 1.0g of Chine charcoal formula granules, put them in a conical flask, add 15ml of water to dissolve them ultrasonically, add 5ml of 15% hydrochloric acid, heat and reflux at 110°C for 45 minutes, let cool, and filter; the filtrate is extracted twice with ethyl acetate, 20ml each time, combined ethyl acetate solution, evaporated to dryness; add 1ml methanol to the residue to dissolve, as the test solution. Take another 1.0g of Imperata rhizome reference medicinal material, add 80ml of water, boil for 30 minutes, filter, concentrate the filtrate to 15ml, add 5ml of 15% hydrochloric acid, and prepare the rest with the same preparation method of the test sample as the reference medicinal solution. According to the thin-layer chromatography ("Chinese Pharmacopoeia" 2010 edition, an appendix VIB) test, absorb 5 μl each of the test solution and the control medicinal solution, and spot...

experiment example 3

[0059] TLC Identification of Experimental Example 3 Thuja Charcoal Formula Granules

[0060] Take 1.0g of Arborvitae charcoal formula granules, put them in a conical flask, add 25ml of water to dissolve them ultrasonically, add 3ml of 15% hydrochloric acid, heat in a water bath at 100°C for 30 minutes, let cool, and filter; the filtrate is extracted twice with ethyl acetate, Each 25ml, combined ethyl acetate solution, evaporated to dryness, the residue was dissolved in 1ml of methanol, as the test solution. Take another 2.0g of Arborvitae leaves as reference medicinal material, add 80ml of water, boil for 30 minutes, filter, concentrate the filtrate to 25ml, add 3ml of 15% hydrochloric acid, and prepare the rest as the reference medicinal material solution by the same preparation method as the test sample. According to the thin-layer chromatography ("Chinese Pharmacopoeia" 2010 edition, an appendix VIB) test, draw 5 μl of each of the above two solutions, and respectively spo...

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Abstract

The invention discloses a thin-layer chromatography identification method of carbon traditional Chinese medicine formula granules. The method comprises the steps that 1, sample powder is taken and dissolved through water, hydrochloric acid is added, heating is conducted for hydrolysis, filtration is conducted, filtrate is extracted through ethyl acetate or ethyl ether, extract liquor is evaporated to dryness, and residues are dissolved through methyl alcohol to serve as a test sample solution; 2, reference medicinal materials are taken, decocted through water and filtered, filtrate is concentrated, and then a reference medicinal material solution is prepared through the method for preparing a test sample; 3, the test sample solution and the reference medicinal material solution are sucked and dripped on a same silica gel GF254 thin-layer plate, and developing is conducted by taking a mixture of methylbenzene, one or more of ethyl formate or ethyl acetate or butyl acetate and formic acid as developing solvents according to the volume ratio of 2-8 to 2-8 to 0.1-1; 4, the thin-layer plate is viewed under a 254 nm ultraviolet lamp, and principal spots with the same color are showed on the positions, corresponding to the chromatography of the reference medicinal materials, in the test sample chromatography. The method is rapid, comprehensive and high in specificity, and the requirements of carbonized herbs and the carbonizing drug characteristic of the formula granules thereof can be embodied.

Description

technical field [0001] The invention belongs to the technical field of quality detection of traditional Chinese medicine formula granules, and in particular relates to a TLC identification method for charcoal traditional Chinese medicine formula granules. Background technique [0002] Traditional Chinese medicine is the treasure of the Chinese nation, and the use of charcoal from traditional Chinese medicine as medicine has national characteristics. The use of charcoal decoction pieces has a history of thousands of years, and it is still frequently used in clinical practice. Traditional Chinese medicine charcoal requires "fried charcoal to preserve its properties", but the quality of charcoal decoction pieces on the market is uneven, and the degree of processing can vary from light to heavy. There is also a lack of identification items for charcoal decoction pieces in the Pharmacopoeia, which greatly restricts the use of charcoal decoction pieces. [0003] At present, the r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90
Inventor 谭登平程学仁朱德全林振东刘丽萍王闽予陈向东
Owner GUANGDONG YIFANG PHARMA
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