The invention relates to a 3D printing composition as well as a preparation method and applications thereof. The composition comprises gypsum powder, oxide powder, a binding material, a fortifier, a solid lubricant and a preservative, and preferably comprises auxiliaries. The composition is low in cost, good in stability, high in forming strength, good in tenacity, difficult to deform, can be used in the field of 3D printing technology, and the preparation method has the advantages of being pollution-free, green, environment-friendly and the like. Therefore, the 3D printing composition as well as the preparation method and applications thereof have excellent scientific research values and industrial application potential.