The invention discloses a preparation method of
ceramide. The preparation method comprises the following steps: step 1, adding
ethanol into konjac flying
powder, carrying out stirring and filtering toobtain an
ethanol extracting solution and a filter residue for later use; step 2, concentrating the
ethanol extracting solution to obtain a concentrated paste; step 3, adding
potassium hydroxide intothe concentrated paste, carrying out stirring to obtain a stirred liquid, adding
petroleum ether into the stirred liquid, and carrying out extracting and concentrating to obtain a crude
ceramide product; and step 4, adding an
organic solvent into the crude
ceramide product for
dissolution, carrying out eluting by adopting an eluting agent to obtain an eluent, and carrying out
drying to obtain theceramide. According to the preparation method, soaking is carried out by using the ethanol for removing peculiar-smell substances in the konjac flying
powder,
solid-liquid separation is carried out by adopting
filtration, then an obtained
solid is dried and used as feed, and an obtained liquid is used for preparing ceramide. Acidic substances such as fatty acids and the like in the crude ceramideproduct is neutralized through the addition of
potassium hydroxide, so that the ceramide is promoted to exist in a
free form, the purity of the prepared ceramide can reach 96% or above, and the use amount of organic reagents is low.