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Method for purifying sesamin and sesamolin

A technology of sesamolin and purification method, which is applied in the direction of anti-toxins, digestive system, metabolic diseases, etc., can solve problems such as complex, unsuitable for industrial application, single active ingredient or purification method, etc., and achieve strong process stability and simple operation Effect

Inactive Publication Date: 2009-12-16
河南省农科院农副产品加工研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a kind of purification method of sesamin and sesamolin, overcome the defect that the existing method can only obtain a single active ingredient or the purification method is complex and unsuitable for industrial application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take 100g of sesame oil extracted with petroleum ether, add 500mL of methanol, reflux and extract at 90°C for 20min, after cooling to room temperature, freeze at -30°C for 8h, triglycerides solidify, and suction filter at -20°C to obtain lignans The extract of the mixture of compounds was removed from methanol under reduced pressure to obtain a light yellow paste.

[0022] Add 20mL of ethanol solution of potassium hydroxide with a mass concentration of 5% to the paste, saponify at 90°C for 10min, add 100mL of water after cooling and transfer to a 500mL separatory funnel, extract with ether, wash with water, anhydrous sodium sulfate Drying, suction filtration and removal of the solvent under reduced pressure yielded a light yellow semi-solid paste.

[0023] Add 20mL of diethyl ether to the light yellow semi-solid paste, shake it fully, seal it and place it at 5°C for 6h, a white powdery sesamin solid is precipitated, and the filtrate and white powdery solid are obtained ...

Embodiment 2

[0025] Take 200mL of commercially available roasted sesame oil, add it to an alumina column (40cm×4cm i.d., 150g of alumina), and elute with petroleum ether, use 2mL of the eluent to add 1mL of concentrated hydrochloric acid containing 2% (wt%) sucrose to heat The reaction was monitored for 5 minutes, and the eluent was replaced with ethyl acetate for elution when a reddish color appeared in the reaction until no color reaction occurred. Ethyl acetate eluate was evaporated under reduced pressure to obtain a yellow semi-solid paste.

[0026] The paste is crystallized and recrystallized successively with ether, chloroform, petroleum ether, ethanol, etc. to obtain 0.68 g and 0.33 g of sesamin and sesamolin with a purity of more than 95 percent, respectively. The ether used was 25mL, the chloroform was 4mL, the ethanol used for the recrystallization of sesamin and sesamolin was 10mL and 6mL respectively, and others were the same as in Example 1.

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Abstract

The invention relates to a method for purifying sesamin and sesamolin in sesame oil, which comprises the steps of: separating lignanoid compounds without fat constituents from the sesame oil first; adding ether into the lignanoid compounds to precipitate the sesamin; performing the filtration; dissolving an obtained filtrate by chloroform after concentration under reduced pressure, adding petroleum ether into the mixture until white opacity appears; heating and dissolving the obtained solution; and standing the obtained solution to precipitate the sesamolin. The method for purifying the sesamin and the sesamolin has the advantages of simple operation, safety, economization, strong process stability and the like, and is particularly suitable for industrialized application; and the method can synchronously obtain high-purity sesamin and sesamolin, and achieve the aim of comprehensive utilization.

Description

(1) Technical field [0001] The invention relates to a method for purifying sesamin and sesamolin in sesame oil. (2) Background technology [0002] Sesamin is a lignan compound mainly present in sesame with the molecular formula C 20 h 18 o 6 , with a melting point of 121-123°C, its content accounts for about 0.2%-0.5% of sesame, and its content is 0.5-1% in non-roasted and unrefined sesame oil. It is one of the important bioactive components in sesame. A large number of in vitro experiments, animal experiments and clinical experiments have proved that sesamin has a wide range of biological activities. It can not only protect and strengthen liver function, regulate blood pressure, lower cholesterol, but also directly act on tissues and organs to exert its excellent antioxidant capacity. In addition to studying its physiological activity and its beneficial effects on the health of the body, some people have also studied the bactericidal effect of sesamin and its stabilizing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/04A61P1/16A61P9/12A61P3/06A61P39/06A61P7/02A61P9/10A61P35/00
Inventor 黄纪念宋国辉孙强詹传保
Owner 河南省农科院农副产品加工研究所
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