The invention relates to a method for extracting rutin from ilex latifolia thumb. The method comprises technical steps: performing continuous reflux treatment to dried pulverized ilex latifolia thumb with acetone in a water-bath at 50-60 DEG C for 2-4 h, recycling acetone, airing a sample, taking 70-80% of ethanol as an extraction medium with the weight volume ratio of material to liquid being 1:(20-30), performing ultrasonic extraction and filtering, carrying out pressure reduction to recycle ethanol, continuously performing ultrasonic treatment to mother liquor for 1 h, performing cooling and standing for crystallization, performing pumping filtration to obtain a crude rutin product, performing continuous reflux treatment to the crude rutin product with acetone for 0.5-1 h, recycling acetone, drying the sample, adding distilled water at a weight volume ratio of 1:100 for heating and dissolving the sample, performing filtering while the obtained product is hot, and performing cooling and standing for crystallization to obtain a fine rutin product. Through adoption of ultrasonic-assisted extraction technology, the extraction efficiency and yield are improved. The method is simple in operation process, allows ilex latifolia thumb resources which are abundant in the southwest region of China to be fully used, develops a new field of ilex latifolia thumb application, and solves the problem that a rutin raw material is tight.