84results about How to "Avoid biotoxicity" patented technology

The invention belongs to the field of nano material preparation, and particularly relates to water-soluble fluorescent magnetic nano particles and a preparation method thereof. The invention adopts the technical scheme that the method comprises the following steps of: preparing magnetic nano particles by adopting a solvent thermal method, coating a SiO2 shell layer on the surface of the magnetic nano particles by hydrolysis of ethyl orthosilicate under the alkali condition, and connecting -NH2 to the outermost layer through a silane coupling agent with amino; and finally, mixing two kinds of aqueous solution, adding a connecting agent such as ethylene dichloride (EDC) or the like, and connecting the magnetic nano particles and quantum dots through chemical bonds formed by condensation reaction between amino and carboxyl to obtain the fluorescent magnetic nano particles. The fluorescent magnetic nano particles obtained by the technical scheme have low toxicity, good stability and magnetic response and excellent fluorescence; and the method has short experimental period, simple and safe operation and low raw material cost, can be completed in a common chemical laboratory, and lays a foundation for wide popularization and application.

The invention discloses a method for in-situ preparing antibacterial supermolecule aquogel. The method comprises the following operation steps: silver nitrate crystal and polyethyleneglycol-polypropylene glycol-polyethyleneglycol triblock copolymer solution are mixed and stirred to obtain mixed solution; the steps of stirring the mixed solution for 15 minutes and ultrasonically dispersing the mixed solution for 5 minutes are alternatively carried out for 2-4 hours to obtain nano silver sol; and the nano silver sol and Alpha-cyclodextrin solution are mixed, stirred and stands at the room temperature to obtain hydrogel. The hydrogel can be used for preparing injectable drug carriers. The invention has the advantages of simple operation, adjustable gel strength and the gelation time, room temperature forming, no involvement of chemical cross linking reaction and the usage of organic solvent, favorable biocompatibility and antibacterial property of obtained gel, and the like and is prospective to be widely applied in the field of biomedical engineering materials.

The invention discloses a tumor-targeting living body multimodality imaging and analyzing method based on gold nano-clusters. According to the invention, through an in-vitro cell (including various tumor cells and normal cells) experiment, related cells and chlorauric acid with a certain concentration and a salt solution thereof are incubated under a physiological condition, such that gold nano-clusters are produced; and real-time non-invasive high-resolution tumor cell fluorescence imaging, Raman imaging and ultrasound imaging can be realized. On the basis, a local subcutaneous injection method is adopted, and real-time in-situ living body tumor-targeting fluorescence imaging, Raman imaging and ultrasound imaging are realized on tumor-transplantation nude mice models. With the functional nano-grade probes, multiple functions of tumor targeting, fluorescence imaging, Raman imaging and ultrasound imaging are combined, such that multimodality synchronic monitoring can be realized. Also, with the gold nano-cluster in-situ living body imaging method, accurate positioning and tumor-targeting imaging and analysis can be realized.

The invention provides a gold nano-star @ quantum dot composite cell probe with photothermal and fluorescence enhancement dual-functions, which sequentially comprises a gold nano-star, a silica layer, quantum dots and a silica nano-shell layer from inside to outside. The cell probe is prepared by using a chemical layer-by-layer growth method implemented through the following steps of: firstly, preparing a gold nano-star by using a seed crystal growth method; coating a silica layer on the outside of the gold nano-star by means of hydrolysis so as to form a silica sphere coated with the gold nano-star; then, linking quantum dots on the surface of the silica sphere through a crosslinking reaction so as to form a silica sphere composite material internally coated with the gold nano-star and externally linked with the quantum dots; and finally, coating a silica nano-shell layer on the surface of the composite material. Compared with traditional probes, the probe integrates the functions of photothermal treatment and fluorescence labeling; the fluorescence intensity of the quantum dots is enhanced, and the biological toxicities of the gold nano-star and the quantum dots are effectively avoided; and in addition, the cell probe has extremely good biological compatibility.

The invention discloses a making method of aqueous intoxic degraded polyurethane elastomer, which comprises the following steps: heating the polymer diatomic alcohol; dehydrating in the vacuum; cooling; adding diisocyanate and catalyst to prepolymerize; stirring through strong force; adding water to emulsify; cooling after prepolymerizing; adding low-molecular chain-extending agent I to lengthen chain; stirring through strong force; adding water to emulsify; cooling after prepolymerizing; adding low-molecular chain-extending agent II to lengthen chain again; dripping neutral agent to manufacture carboxyl; moulding the emulsion to obtain the product.

The invention provides an anti-interference electrocardiograph detection system, a detection method, a special flexible graphene electrode, and a preparation method and the application of the special flexible graphene electrode. The anti-interference electrocardiograph detection system comprises the flexible graphene electrode, an electrocardiosignal acquiring and processing module, an impedance measurement module, an electrocardiographic wire and a signal processing module, can measure an electrocardiosignal and a motion artifact signal, and removes the motion artifact in the electrocardiosignal. The flexible graphene electrode is formed in a way that a graphene material is adhered on a sponge layer through negative pressure deposition to obtain a graphene-sponge conductive composite, a fastener is additionally added on the graphene-sponge conductive composite, and the electrocardiographic wire is fixedly connected to the graphene-sponge conductive composite through the fastener. The anti-interference electrocardiograph detection system and the detection method effectively overcome the interference of the motion artifact on the electrocardiosignal, and guarantees the instantaneity, the accuracy and the reliability of the electrocardiographic examination result; the preparation flow of the flexible graphene electrode is simple, the biological compatibility of the flexible graphene electrode is good, the service life of the flexible graphene electrode is long, and the flexible graphene electrode can be used in the anti-interference electrocardiograph detection system.

The invention discloses a method for preparing polyethylene glycol network gel by using a mercapto-vinyl clicking system. Free radicals are generated through initiating by using a free-radical initiator under the condition of a certain temperature or lighting, so as to synchronously catalyze a vinyl compound and a mercapto compound to be subjected to click reaction. According to the method disclosed by the invention, by using the controllability of the efficiency of initiation of the free radicals under the condition of temperature and lighting, a polyethylene glycol derivative containing three or more multiterminal unsaturated carbon-carbon double bonds and a polyethylene glycol derivative which at least contains two terminal mercapto groups are synthesized by using the method, so as to prepare a polyethylene glycol polymer gel network with a regular structure. According to the method disclosed by the invention, the operation is simple, reaction materials are easily obtained, reaction is rapid, and the reaction conditions are mild; the polymer network prepared by the method disclosed by the invention has the advantages of good biocompatibility, regular structure, high mechanical strength and the like.

The invention discloses a preparation method of a functional hydrogel medical dressing. The preparation method comprises the following steps: 1. blending a biopolymer material, a chemical synthesized polymer material, a transforming growth factor TGF-beta and distilled water so as to form a physical-crosslinked hydrogel, wherein the biopolymer material consists of carboxymethyl chitosan, chitosan quaternary ammonium salt, gelatin, gellan gum and sodium alginate; and the chemical synthesized polymer material consists of polyvinyl alcohol and polyvinylpyrrolidone; 2. coating and processing the physical-crosslinked hydrogel obtained in the step 1 by virtue of a coating machine; and 3. conducting radiation sterilization on the hydrogel which is coated in the step 2, and implementing radiation crosslinking during the process of radiation sterilization. The method, through physical crosslinking and radiation crosslinking, can form the hydrogen of a dual-network structure; and the hydrogel medical dressing is high in strength and melting-up property, and the dressing is capable of protecting wound surfaces, stopping bleeding, relieving pain, inhibiting bacteria and promoting the growth of epithelial cell.

The invention provides a preparation method of a high-brightness non-toxic fluorescent quantum dot nanometer composite probe. The preparation method includes the steps of preparing silica sphere sol, preparing a silica and quantum dot composite material, and preparing a silica-quantum dot-silica structure. According to the preparation method, a lot of fluorescent quantum dots are connected on the surface of the silica, so that a single probe is provided with a fluorescent enhancement effect dozens or even a hundred of times higher than that of a single quantum dot, then the silica is used for coating silica / quantum dot nanometer particles, the good biocompatibility is achieved, biomolecules are quite easy to link, and the biotoxicity of the quantum dots is shielded thoroughly.

The invention discloses a porphyrin derivative nano-composite preparation applied in photodynamic therapy and photothermal therapy of rheumatoid arthritis and an application thereof. The invention discloses an experimental method of a composite reagent of a water-soluble porphyrin derivative and a nano-material. The composite reagent has stable fluorescence characteristic and excellent biocompatibility. According to the composite reagent, drug release detection and tracing of an inflammation area can be realized, and enrichment in the inflammation area can be achieved through porphyrin molecule absorption of the nano-material. The experimental method provided by the invention is easy to implement, and has low toxicity and high specificity. The porphyrin derivative in the obtained composite reagent can undergo violet light excitation to generate singlet oxygen. The nanometer material in the composite reagent has excellent photothermal effect under the red light excitation condition. Drug enrichment and multi-model treatment by photodynamic therapy and photothermal therapy for inflammation disease can be realized, and the porphyrin derivative nano-composite preparation has wide medical clinical application value and prospect.

The invention discloses a green synthetic method of a fluorescence copper nano-cluster, and an application of the fluorescence copper nano-cluster. The synthetic method concretely comprises the following steps: incubating a certain concentration of a copper salt solution and different kinds of tumor cells, and carrying out in-situ synthesis to obtain a copper nano-cluster having an excellent fluorescence performance in order to realize the real time and high resolution fluorescence imaging of the tumor cells. The copper nano-cluster can realize the rapid real-time in-situ in-vivo tumor targeting fluorescence imaging on a tumor-hearing nude mouse model. The copper nano-cluster is prepared through direct biosynthesis of tumor cells and has a very good biocompatibility; and the method has the characteristics of environmental protection, no pollution to the environment, realization of rapid and accurate positioning and tumor targeting imaging analysis by the in-situ synthesis reaction, simplicity, easy implementation, accuracy and high efficiency.

The invention discloses an electrochemical method for preparing a chitosan gelatin coating on a titanium implant surface. The method comprises the following steps of: preparing mixed electrodeposit liquid containing gelatin chitosan; immersing platinum serving as an anode and a titanium implant serving as a cathode in the electrodeposit liquid to perform an electrodeposition reaction to result in that both chitosan and gelatin in the electrodeposit liquid are deposited on a metallic titanium surface; and taking the obtained product out, clearing the solution attached to the surface and drying the surface. The prepared chitosan gelatin composite coating has the advantages of good bonding with the titanium implant, controllable gelatin content, high biological activity and availability for further loading functional biological molecules.

The invention relates to a preparation method for a core-shell structure drug carrier for real-time monitoring drug release through near-infrared light stimulation. The invention belongs to the field of a biomedical material and specifically relates to the preparation method for the core-shell structure drug carrier for real-time monitoring drug release through near-infrared light stimulation. The invention aims to solve the problem that the drug release platform cannot effectively monitor the release amount of the drug. The method comprises the following steps: 1) synthesizing an up-conversion nanometer particle core; 2) synthesizing an up-conversion nanometer particle coating the outer shell; 3) coating the outer layer of the up-conversion nanometer particle with mesoporous silica; 4) synthesizing 4-oxy-4-(pyrene-4-methoxy) butyrate; 5) performing functional decoration on the nanometer particle surface; 6) carrying drug on the nanometer particle and plugging the duct. The preparation method is applied to the cancer therapy and can realize the real-time monitoring for anti-cancer drug release.

The invention discloses a reduced glutathione freeze-dried powder injection and a preparation method thereof. The powder injection is mainly made of the reduced glutathione and other proper accessories with specifications of 0.3 g (2 mL) per injection, 0.6 g (4 mL) per injection, 0.9 g (6 mL) per injection, 1.2 g (8 mL) per injection, 1.5 g (10 mL) per injection, and 1.8 g (12 mL) per injection. As the reduced glutathione freeze-dried powder injection of the invention uses folic acid as an antioxidant, a toxic side effect on human body caused by using sodium sulfite and sodium metabisulfite as the antioxidant can be avoided. Simultaneously, the folic acid can enhance human immunity so as to play a better efficacy. The reduced glutathione freeze-dried powder injection of the invention has good safety and efficacy. The preparation process thereof is simple, and good in quality controllability and stability.

The invention belongs to the technical field of biomedical materials and discloses a modified polyvinyl alcohol cornea repair material with collagen in graded distribution and a preparation method. The preparation method comprises the steps of preparing polyvinyl alcohol into a polyvinyl alcohol solution by utilizing deionized water, performing defoaming and then pouring the polyvinyl alcohol solution in a die to be laid flatly and frozen; evenly coating the surface of the frozen polyvinyl alcohol solution with a collagen solution under the conditions of room temperature, performing unfreezing at room temperature, and obtaining a compound system of polyvinyl alcohol and collagen; repeating the freezing and unfreezing process of the compound system 7-10 times, finally performing natural air drying, and obtaining the modified polyvinyl alcohol cornea repair material with collagen in graded distribution. The content of collagen in the cornea repair material is in graded distribution, biological activities on the two sides of the material are different, the requirements of a cornea layered structure are met in function, and the modified polyvinyl alcohol cornea repair material and the preparation method have good application prospects.

The invention discloses composition based on main components of Pu'er tea and the application thereof. The composition comprises tea brown pigment, tea polysaccharide, tea polyphenol, caffeine, gamma-aminobutyric acid and gallic acid. A wild type mouse embryo fibroblast is utilized to test the killing effect of DNA toxicity chemical substance to normal cells and the effect that the composition provided by the invention protects the normal cells from DNA damage and prevents apoptosis; the result shows that the composition provided by the invention can effectively prevent the normal cell death caused by DNA toxicity chemical substance; and further research shows that the composition provided by the invention does not protect tumor cell death caused by the DNA toxicity chemical compound, conversely, the composition provided by the invention can promote the killing effect of the DNA toxicity chemical compound to the tumor cells. Therefore, the composition can be used for protecting the normal cells during the chemotherapy of tumor patients and alleviating toxic side effects caused by tumor chemotherapy, and meanwhile sensibilizes chemotherapy drug, enhances chemotherapy effect, and improves survival quality of chemotherapy patients.

The present invention discloses an inorganic silica / organic nano-particle core-shell structure preparation method, wherein a surfactant hexadecyl trimethyl ammonium bromide is adopted to stabilize nano-particles, and is adopted as template for porous silica shell layer forming, and a silane coupling agent is subjected to hydrolysis compression at the template position under an alkaline environment so as to achieve coating of inorganic porous silica on the surface of organic nanoparticles. According to the present invention, a thin layer silicon shell is introduced to the surface of organic nanoparticles, such that the surface modification operation is diversified; the preparation method has a universality characteristic, and is applicable for a series of hydrophobic fluorescence dyes; and the preparation method has characteristics of simple and easy-performing operation process, good repeatability, mild reaction process and universality so as to provide promotion use values.

The invention discloses a preparation method of a high drug loading rod-shaped nanometer drug for cancer treatment, wherein an oversaturation degree of a hydrophobic anti-cancer drug in water, and a nanometer fiber suspension ultrasonic time are controlled, and a hydrophobic interaction between an amphiphilic surfactant and the surface of a rod-shaped nanometer drug is adopted to modify the surface of the nano-rod so as to achieve preparation of high drug loading hydrophobic rod-shaped nanometer drug with characteristics of cancer treatment and stable bioenvironment. With the technical scheme, the preparation method is simple, experimental basis is provided for preparation of other high drug loading hydrophobic anti-cancer non-spherical nanometer materials with characteristics of cancer treatment and stable bioenvironment, and wide applications can be provided for the fields of biology, medicine, chemistry and the like.

The rolling artificial hip joint as one kind of artificial bone joint includes femoral head part, rolling part, prosthesis handle part and circular screw nut. The rolling part consists of two rolling bearings separated with two spacing rings, and the rolling bearings have their inner rings matched with the main shaft of the prosthesis handle part and outer rings fitted with the inner ring of connecting part in the femoral head part. By means of the rolling bearings, the present invention changes the sliding friction between the artificial semi-hip joint and the natural cotyloid cavity into rolling friction in bending-stretching motion direction, and this reduces the wear of prosthesis greatly, prolongs the service life, lowers the production cost and avoids the toxic action of polyethylene and meets the treating requirement of patient.

The invention discloses a method for synthesizing aromatic polyester without a catalyst. The method comprises the following steps: a pre-polymerization stage: under the condition of no additional catalyst, carrying out esterification reaction on aromatic dicarboxylic acid and excessive molar ratio of dihydric alcohol to obtain a hydroxyl-terminated prepolymer; then adding cyclized dicarboxylic acid or corresponding anhydride with the molar weight greater than that of the hydroxyl-terminated prepolymer, and continuously carrying out esterification reaction to obtain a carboxyl-terminated prepolymer; and a polymerization stage: under the condition of reduced pressure, controlling the reaction temperature to be not lower than the boiling point of anhydride corresponding to the cyclized dicarboxylic acid, and removing anhydride corresponding to the cyclized dicarboxylic acid from the carboxyl-terminated prepolymer to obtain the aromatic polyester. The synthesis method provided by the invention can be used for preparing aromatic polyester without catalyst residues, so that the problems of biotoxicity, accelerated aging and the like caused by use of the catalyst are avoided, and the method can be safely applied to the fields of food contact materials, medical materials and the like.

The invention discloses a carbon-encapsulated carbon-gadolinium quantum dot and a preparation method thereof. The carbon-encapsulated carbon-gadolinium quantum dot is a quantum dot with a core-shell structure and takes a carbon-gadolinium quantum dot with a face-centered cubic structure as a core; the core is encapsulated with a carbon film used as a shell; the carbon-encapsulated carbon-gadolinium quantum dot is obtained through laser irradiation. According to the carbon-encapsulated carbon-gadolinium quantum dot and the preparation method thereof, the carbon-gadolinium quantum dot with the face-centered cubic structure is prepared for the first time and carbon encapsulation is successfully carried out on the surface of the carbon-gadolinium quantum dot; laser power and irradiation time adopted in a preparation process have good controllability and the preparation process can be carried out at room temperature; the quantum dots with different grain diameters can be obtained accordingto parameters including laser bombardment time, light power and the like.

The present invention belongs to the field of artificial joint. The rolling artificial knee joint includes thigh part, roller, spindle and tibia part. The connection mode includes via the two roller tracks in the thigh part and the two roller tracks in the spindle to form one ring space, setting the roller in the ring space, connecting the libia part and the spindle, setting the spindle in the internal and external condyles and forming two rolling bearing-like structures with the thigh part, roller and spindle. The present invention has reduced prosthesis wear, prolonged use period, low production cost and no biological toxic action of polyethylene and can meet the treatment requirement of patient.

The invention relates to the field of high-molecular porous materials and particularly relates to a preparation method of porous chitosan-based microspheres for medicine carriers, adsorption and separation, hemostasis and wound dressing. The preparation method comprises the following steps: (a) respectively preparing solidification liquid, an emsulsifying-agent solution and a diluted-acid solution of pelletizing materials and precooling the solidification solution to minus 20 DEG C; (b) dripping the chitosan diluted-acid solution into the emsulsifying-agent solution, homogenizing and stirring to form emulsion; and (c) carrying out thermally-induced phase separation on the emulsion under the temperature ranging from minus 60 DEG C to minus 10 DEG C, adding precooled solidification liquid, carrying out reverse-phase regeneration and thus obtaining the chitosan-based porous microspheres. The preparation method has the beneficial effect that the surfaces and the inner parts of the porous microspheres have porous structures with communicated heights.

The invention discloses an insect-resistant fusion gene, a protein encoded by the insect-resistant fusion gene, as well as an expression vector and application of the insect-resistant fusion gene; andthe purpose of the invention is to solve the technical problem of irresistible trend of drug resistance of insects to pesticides. According to the invention, an insect-resistant fusion gene and an insect-resistant fusion gene CryAb-VIP3A are designed. The invention also provides a protein encoded by the insect-resistant fusion gene CryAb-VIP3A, and designs an expression vector constructed by using the insect-resistant fusion gene CryAb-VIP3A as well as a recombinant bacterium constructed by using the expression vector. The invention further discloses application of the insect-resistant fusiongene CryAb-VIP3A in preparation of insect-resistant plant cells. The invention still further discloses application of the protein encoded by the insect-resistant fusion gene CryAb-VIP3A in preparation of pesticides. The insect-resistant fusion gene CryAb-VIP3A disclosed by the invention can be stably expressed in corn so as to avoid biological toxicity caused by transferring of an original VIP3A(a) gene into the plant alone and overcome the phenomenon of gene silencing in foreign gene expression, so that more choices are provided for plant pest control; and thus, a new method is provided forkeeping down the trend of the drug resistance of the insects to the pesticides.

The invention discloses semi-aromatic polyimide, a semi-aromatic polyimide-based color-adjustable room-temperature phosphorescent material and a preparation method of the semi-aromatic polyimide-based color-adjustable room-temperature phosphorescent material. The chemical structural formula of the semi-aromatic polyimide is shown in the specification. The obtained semi-aromatic polyimide has high thermal stability and high mechanical property, and the light transmittance reaches 80% or above. The semi-aromatic polyimide-based room-temperature phosphorescent material is prepared by doping polyimide with other small organic molecules, and regulation and control of phosphorescent life and phosphorescent color can be realized. The organic metal complex-free pure organic photoluminescence polymer phosphorescent material avoids potential biotoxicity, and has a wide effect in the fields of anti-counterfeiting, information encryption, biological imaging and the like.

The invention discloses preparation of a high drug loading multifunction nanometer drug with a combination treatment effect, wherein an oversaturation degree of a hydrophobic anti-cancer drug in water, a cation type surfactant concentration, and the amount of an added Au seed are controlled, and a chemical effect between a disulfide bond in a hydrophilic surfactant and the surface of a Au shell is adopted to carry out hydrophilic modification on the surface of a nanometer drug so as to achieve preparation of high drug loading multifunction nanometer drug with characteristics of chemotherapy and thermotherapy combination treatment effect and stable bioenvironment, wherein a layer of a Au shell grows on the nanometer drug. With the technical scheme, the preparation method is simple, experimental basis is provided for preparation of other multifunction nanometer materials for cancer treatments, and wide applications can be provided for the fields of biology, medicine, chemistry and the like.

The invention discloses a reagent for early rapid detection of Alzheimer's disease and an image preparation for multi-mode imaging of the early rapid detection. The reagent consists of transition metal ions and noble metal ions which have excellent biocompatibility, and nano-clusters and porphyrin derivatives of the transition metal ions and noble metal ions. An imaging detection reagent which can be used for accurately targeting and rapidly marking relative focus positions can be synthesized in fixed points by virtue of in-situ biological action, so that highly-sensitive and rapid tracing and monitoring with non-invasive, real-time and dynamic modes can be achieved; and moreover, the reagent cannot cause biologically toxic or side effects, and is simple, convenient and feasible in detection method. By adopting the medicines disclosed by the invention, the early, rapid and multi-mode (such as CT, ultrasound, fluorescence and MRI) imaging detection of the Alzheimer's disease can be achieved; and the medicines have wide medical application prospects.

The invention discloses a preparation method for a polyhydroxy functional POSS hybrid material. The preparation method is characterized by the following steps: adding reactants, namely, polyethylene glycol derivative PEG (C=CH2) containing unsaturated carbon-carbon double bond at one end, olefinic organism (RHC=CH2), octa-sulfydryl POSS and dissolvent into a reactor; adding radical initiator afterthe added reactants dissolve and uniformly disperse; reacting for 40min-4h under the nitrogen shielding condition at 30-80 DEG C or under an ultraviolet light condition, thereby acquiring the POSS hybrid material; removing the remained radical initiator and unreacted reactants, and drying, thereby acquiring the polyhydroxy functional POSS hybrid material. The preparation method disclosed by the invention has the advantages of simplicity in operation, easily acquired reaction materials, fast reaction and mild reaction condition. The polyhydroxy functional POSS hybrid material prepared according to the invention has the advantages of high biocompatibility, controllable hydrophily and hydrophobicity, controllable form under condensed state, multifunctional reaction group and the like.