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High-brightness non-toxic fluorescent quantum dot nanometer composite probe and preparation method thereof

A fluorescent quantum dot and nanocomposite technology, which is applied in the field of fluorescent quantum dot nanocomposite probes and their preparation, can solve problems such as poor biocompatibility and biotoxicity, and achieve good biocompatibility, good biocompatibility, The effect of preventing the fluorescence quenching effect

Inactive Publication Date: 2013-01-02
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional quantum dot fluorescent probes have fatal weaknesses such as poor biocompatibility and biotoxicity, so the design of a high-brightness fluorescent probe with high biocompatibility and low biotoxicity is imminent

Method used

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  • High-brightness non-toxic fluorescent quantum dot nanometer composite probe and preparation method thereof
  • High-brightness non-toxic fluorescent quantum dot nanometer composite probe and preparation method thereof
  • High-brightness non-toxic fluorescent quantum dot nanometer composite probe and preparation method thereof

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Effect test

Embodiment 1

[0035] (1) Hydrolyze 2ml of tetraethyl orthosilicate (TEOS) under the catalysis of 4ml of ammonia water, and react at 40°C for 4 hours to prepare a silica sol with a diameter of 80nm.

[0036] (2) The prepared silica sol was modified with 200 microliters of 3-aminopropyltrimethoxysiloxane (APTMS) to bring amino groups on its surface, and then centrifuged to remove excess APTMS, Then 5ml concentration is that the MPA stabilized water-soluble fluorescent CdTeS quantum dot of 2M is added in the silica sol that has been modified, reacts 24 hours and makes it react fully, centrifuges then, and centrifugal product is dispersed in ethanol (the ethanol consumption is able to Just disperse the centrifuged product).

[0037] (3) Add 2ml of ammonia water and 1000 microliters of TEOS to the composite material prepared in (2). TEOS is hydrolyzed under the catalysis of ammonia water. The reaction condition is to react at room temperature for 24 hours. After the reaction, use γ-mercaptopropy...

Embodiment 2

[0040] (1) Hydrolyze 4.4ml of tetraethyl orthosilicate (TEOS) under the catalytic action of 8ml of ammonia water, and react at 80°C for 2 hours to prepare a silica sol with a diameter of 150nm.

[0041] (2) The prepared silica sol was modified with 500 microliters of aminopropyltriethoxysilane to bring amino groups on its surface, followed by centrifugation to remove excess APTMS, and then 10ml of 2M The MPA stabilized water-soluble fluorescent CdTe quantum dots were added to the modified silica sol, reacted for 24 hours to fully react, and then centrifuged and dispersed in isopropanol.

[0042] (3) Add 4ml of ammonia water and 5000 microliters of TEOS to the composite material prepared in (2). TEOS is hydrolyzed under the catalysis of ammonia water. The reaction condition is to react at room temperature for 24 hours. After the reaction, use 3-aminopropyl Trimethoxysiloxane (APTMS)) modified with amino groups on the surface. (4) Add antibodies to the quantum dot fluorescent c...

Embodiment 3

[0044](1) Hydrolyze 0.55ml of tetraethyl orthosilicate (TEOS) under the catalytic action of 2ml of ammonia water, and react at 25°C for 12 hours to prepare a silica sol with a diameter of 110nm.

[0045] (2) The prepared silica sol was modified with 100 microliters of 3-aminopropyltrimethoxysiloxane (APTMS) to bring amino groups on its surface, and then centrifuged to remove excess APTMS, Then 3ml of MPA-stabilized water-soluble fluorescent Mn-doped ZnS quantum dots with a concentration of 2M was added to the modified silica sol, reacted for 24 hours to fully react, and then centrifuged and dispersed in ethanol.

[0046] (3) Add 1ml of ammonia water and 500 microliters of TEOS to the composite material prepared in (2). TEOS is hydrolyzed under the catalysis of ammonia water. The reaction condition is to react at room temperature for 24 hours. After the reaction, use Modified silane coupling agents (such as 3-aminopropyltrimethoxysiloxane (APTMS)) make the surface with amino gr...

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Abstract

The invention provides a preparation method of a high-brightness non-toxic fluorescent quantum dot nanometer composite probe. The preparation method includes the steps of preparing silica sphere sol, preparing a silica and quantum dot composite material, and preparing a silica-quantum dot-silica structure. According to the preparation method, a lot of fluorescent quantum dots are connected on the surface of the silica, so that a single probe is provided with a fluorescent enhancement effect dozens or even a hundred of times higher than that of a single quantum dot, then the silica is used for coating silica / quantum dot nanometer particles, the good biocompatibility is achieved, biomolecules are quite easy to link, and the biotoxicity of the quantum dots is shielded thoroughly.

Description

technical field [0001] The invention belongs to the field of fluorescent probe materials, in particular to a nano-composite probe with high brightness and non-toxic fluorescent quantum dots and a preparation method thereof. Background technique [0002] Fluorescent probes have been widely used in the fields of optical materials, chemical recognition, biological monitoring and sensing, and have become a major technical means to successfully realize the above functions. However, there are also some insurmountable defects in fluorescent probes based on traditional organic fluorescent dyes. In recent years, due to the excellent spectral characteristics and photochemical stability of quantum dots, the study of quantum dot fluorescent probes has attracted extensive attention from researchers in various disciplines at home and abroad. The research fields involve physics, chemistry, life sciences and other disciplines. It has developed into a new interdisciplinary subject. However...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/88C09K11/57C09K11/56
Inventor 尹乃强许小亮朱立新
Owner UNIV OF SCI & TECH OF CHINA
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