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Green synthetic method of fluorescence copper nano-cluster, and application of fluorescence copper nano-cluster

A fluorescent copper nanocluster and green synthesis technology, applied in nanotechnology, nanotechnology, fluorescence/phosphorescence, etc., can solve the problems of low signal enhancement in vivo, poor probe stability, poor probe transportation, etc., to achieve Rapid and precise positioning and tumor-targeted imaging analysis, realization of positioning and tumor-targeted imaging analysis, and the effect of real-time in situ tumor-targeted fluorescence imaging

Inactive Publication Date: 2014-05-28
SOUTHEAST UNIV
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Problems solved by technology

We also encountered many difficulties in the development of cancer-specific imaging reagents, including: poor transport of probes targeting tissue or tumors; high biological toxicity; poor stability of probes; low signal enhancement in vivo, etc.

Method used

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  • Green synthetic method of fluorescence copper nano-cluster, and application of fluorescence copper nano-cluster
  • Green synthetic method of fluorescence copper nano-cluster, and application of fluorescence copper nano-cluster

Examples

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example 1

[0034] Example 1 Method and application based on in situ biosynthesis of copper nanoclusters in tumor cells

[0035] 1. Taking HepG2 cells as the research object, incubate sterile 0.0001mmol / L~1 mmol / L copper salt solution with HepG2 cells in logarithmic growth phase for 8~24 hours (37 °C, 5 % CO 2 , RH 95%), the copper nanoclusters biosynthesized in situ in the cell can be obtained.

[0036] 2. Qualitative and quantitative analysis of copper compound nanoclusters with confocal fluorescence microscopy;

[0037] Liver cancer cells (HepG2) were selected as the research object, and the experimental group divided the liver cancer cells (HepG2) in the logarithmic growth phase at a ratio of 1.6×10 5 The density of cells / well was inoculated in 6-well plates, and after 24 h of culture, a copper salt solution with a concentration of 0.0001 mmol / L-1 mmol / L was added which had been sterilized and diluted with fresh sterile DMEM medium.

[0038] In the control group, the liver cancer...

example 2

[0039] Example 2Tumor-targeted imaging based on in situ growth of copper nanoclusters in living lesions

[0040] Firstly, 0.1-0.5 mL sterile copper salt solution with a concentration of 0.1-100 mmol / L was subcutaneously injected locally near the tumor of nude mice implanted with liver cancer tumor model. After incubation at different time points such as 1 hour, the experimental nude mice were anesthetized with 5% isoflurane gas, and then placed on the operating platform of the small animal in vivo imager, and the images of the tumor area could be collected by selecting blue light excitation.

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Abstract

The invention discloses a green synthetic method of a fluorescence copper nano-cluster, and an application of the fluorescence copper nano-cluster. The synthetic method concretely comprises the following steps: incubating a certain concentration of a copper salt solution and different kinds of tumor cells, and carrying out in-situ synthesis to obtain a copper nano-cluster having an excellent fluorescence performance in order to realize the real time and high resolution fluorescence imaging of the tumor cells. The copper nano-cluster can realize the rapid real-time in-situ in-vivo tumor targeting fluorescence imaging on a tumor-hearing nude mouse model. The copper nano-cluster is prepared through direct biosynthesis of tumor cells and has a very good biocompatibility; and the method has the characteristics of environmental protection, no pollution to the environment, realization of rapid and accurate positioning and tumor targeting imaging analysis by the in-situ synthesis reaction, simplicity, easy implementation, accuracy and high efficiency.

Description

technical field [0001] The invention relates to a green synthesis method of fluorescent copper nanoclusters and its application. Specifically, a copper salt solution of a certain concentration is incubated with different types of tumor cells to synthesize copper nanoclusters with excellent fluorescent properties in situ, thereby achieving the realization of Real-time, high-resolution fluorescence imaging of tumor cells. At the same time, the copper nanocluster of the present invention can realize rapid and real-time in situ tumor-targeted fluorescent imaging in a tumor-bearing nude mouse model. The copper nanocluster of the present invention is directly biosynthesized by tumor cells, and has good biocompatibility; the method is green and environmentally friendly, has no pollution to the environment, and its in-situ synthesis reaction can realize rapid and precise positioning and tumor-targeted imaging Analysis has the characteristics of simplicity, precision and efficiency. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/58A61K49/00G01N21/64B82Y30/00B82Y40/00
Inventor 王雪梅叶静陈东华王建玲
Owner SOUTHEAST UNIV
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