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38results about How to "Act as a protective layer" patented technology

Air spring formulation for automobile

The invention discloses an air spring formulation for an automobile, which is characterized by comprising the following compositions by mass portion: a, the formulation of inner and outer layer rubber of the air spring: 50 to 100 portions of neoprene, 50 to 100 portions of natural rubber, 0.5 to 5 portions of magnesia, 0.5 to 3 portions of stearic acid and 0.5 to 2.5 portions of antiaging agent OD, and b, the formulation of cord fabric rubber of the air spring: 50 to 100 portions of the natural rubber, 0.5 to 2 portions of the stearic acid, 2 to 5 portions of zinc oxide, 0.5 to 3 portions of antiaging agent RD, 0.5 to 2.5 portions of antiaging agent 4020 and 2 to 4 portions of tackifying resin A-90. The air spring manufactured by the formulation can meet the following key technical points: 1, the outer layer rubber has excellent ozone aging resistant property, thermal oxygen aging resistant property and flexing cracking resistant property; 2, the cord fabric rubber is closely adhered with nylon cord fabric, and the rubber has excellent thermal oxygen aging resistant property; 3, the layers are closely adhered; 4, the airtightness is excellent; and 5, the service life is long.
Owner:SHANDONG MEICHEN ECOLOGY & ENVIRONMENT CO LTD

Polyphosphoester containing fluorine and silicon as well as preparation method and application thereof

The invention discloses a polyphosphoester containing fluorine and silicon as well as a preparation method and an application thereof. The chemical formula structure of the polyphosphoester containing fluorine and silicon is expressed as a formula which is shown in description, wherein R1 is selected from a chemical formula which is shown in description, a chemical formula which is shown in description or a chemical formula which is shown in description; R2 is selected from a chemical formula which is shown in description or a chemical formula which is shown in description; n is 10-100. According to the polyphosphoester containing fluorine and silicon, phosphorus, fluorine and silicon elements are used for resisting flame; the phosphorus element is capable of catalyzing a system to form a phosphorus-enriched carbon layer, so that the effect of a protection layer is achieved, and thus the further decomposition of epoxy resin is prevented; silicon-containing epoxy resin can form a carbon layer containing silicon dioxide in the combustion process, so that the carbon layer structure is enhanced, and thus the protection effect of the carbon layer is further improved; through introduction of the fluorine element, the heat stability of the epoxy resin can be improved, so that the flame resistance of the system is improved.
Owner:XIAMEN UNIV

Hollow sphere adopting metal oxide/Pt/C three-layer nano structure and preparation method of hollow sphere

The invention discloses a hollow sphere adopting a metal oxide / Pt / C three-layer nano structure. A spherical shell of the hollow sphere sequentially comprises a metal oxide layer, a Pt layer and an amorphous carbon layer from inside to outside. A template adsorption method is used, a hollow metal oxide sphere is prepared firstly, Pt particles are prepared on the surface of metal oxide with an impregnation method, finally, the surface of the hollow metal oxide sphere coated with the Pt particles is coated with amorphous carbon through hydro-thermal treatment, and then the hollow sphere adopting the metal oxide / Pt / C three-layer nano structure is prepared. The prepared hollow sphere with the metal oxide / Pt / C three-layer nano structure has a regular core-shell structure, the thickness is adjustable, and the specific surface area is larger than 200 m <2> / g. A method is simple, the cost is lower, industrial production can be easily realized, and the hollow sphere can be well applied to fields such as photocatalytic decomposition of water for hydrogen production, gas sensing, super capacitors, lithium ion batteries and the like.
Owner:ZHEJIANG UNIV

Preparation method of modified polyether sulfuryl hydrophobic film for gas-liquid film contactor

The invention relates to a preparation method of a modified polyether sulfuryl hydrophobic film for a gas-liquid film contactor. According to the method provided by the invention, the polyether sulfuryl film is modified in a hydrophobic manner by virtue of combination of sol-gel and a fluorination method, and gases are removed and absorbed under the support of the gas-liquid film contactor. The modifying method provided by the invention comprises the following preparation processes: mixing absolute ethyl alcohol, ammonia water and silicate ester in a certain proportion and stirring to obtain a sol-gel liquid and carry outing surface modification on the polyether sulfuryl film; modifying the modified base film by using fluorine-containing siloxane with low surface energy to obtain the modified polyether sulfuryl hydrophobic film. The film has excellent performances such as high temperature resistance, corrosion resistance and good ventilation effect, and can be used in the gas-liquid film contactor to remove carbon dioxide, sulfur dioxide and smoke with complex components.
Owner:TIANJIN POLYTECHNIC UNIV

Transparent conducting solution and preparation method thereof, color film substrate and manufacturing method

The embodiment of the invention provides a transparent conducting solution and a preparation method thereof, a color film substrate and a manufacturing method, and relates to the field of liquid crystal displays. Equipment for sputtering an indium tin oxide (ITO) is not required any more in a production line of manufacturing the color film substrate, so that the cost is reduced. The transparent conducting solution comprises the following components in parts by weight: 15 to 30 parts of epoxy acrylic resin, 15 to 30 parts of polyurethane acrylic resin, 10 to 20 parts of polyaniline, 3 to 5 parts of photo-initiator, 15 to 25 parts of monomer, and 8 to 10 parts of filler.
Owner:BOE TECH GRP CO LTD

Biological glue type seed coating agent, its prepn. method and use

InactiveCN1784981ASolve the repellent effectSolve gelationBiocideAnimal repellantsOrganic chemistryBiological glue
A seed coating is proportionally prepared from hydroscopic resin, disintegrant, bentone, talc powder, biocolloid, adhesive and porous zeolite powder.
Owner:鄂尔多斯市碧森种业有限责任公司 +1

Anti-saline alkali corrosion resistance-reduction combined grounding body

The invention relates to an anti-saline alkali corrosion resistance-reduction combined grounding body which is characterized by mainly comprising an inner core of galvanized flat steel and a conductive concrete outer cladding, wherein the conductive concrete outer cladding is claded around the inner core; the fixed joint tip of the inner core extends and is exposed out of the outer end surface of the outer cladding. The invention has the advantages that the grounding body is a model integrating the three methods. Conductive cement filled around the grounding body isolates a grounding conductor and saline alkali soil and plays a role of a protecting layer; the conductive cement filled between the grounding conductor and the soil plays a role of a physical resistance reducer; and by adopting a grounding net to make the original grounding body which is easily corroded by saline alkali become into the anti-saline alkali corrosion grounding body after the conductive cement is filled around the grounding body, the corrosion resistance of the grounding conductor is improved by 10 times and the through-current and discharge capacity are also improved, thereby achieving the dual purposes of resisting corrosion and reducing grounding resistance.
Owner:天津市普瑞电力科学研究有限责任公司

Cooker, cooking utensil assembly and manufacture method of cooker

The invention provides a cooker, a cooking utensil assembly and a manufacture method of the cooker. The cooker comprises an iron base body, a first aluminum oxide layer and a non-stick coating. The non-stick coating is attached to the inner wall surface of the iron base body. The non-stick coating is sprayed on a layer surface of the first aluminum oxide layer. Advantages of aluminum oxide such asgreat hardness, compact structure, wear resistance and corrosion resistance are made full use of because the inner wall surface of the iron base body is equipped with the first aluminum oxide layer as an interlayer. Therefore, bonding force between the non-stick coating and the iron base body is improved. The non-stick coating is more firmly attached to the iron base body and used for longer service lifetime. Pollution to the environment is avoided due to phosphorization or oxide treatment by avoiding the defect in the prior art that phosphorization or oxide treatment is needed by the inner wall surface of the iron base body prior to spraying of the non-stick coating.
Owner:FOSHAN SHUNDE MIDEA ELECTRICAL HEATING APPLIANCES MFG CO LTD

Negative temperature coefficient thermistor with nano particle film, and preparation method thereof

The invention discloses a negative temperature coefficient thermistor with a nano particle film, and a preparation method thereof. The thermistor comprises a ceramic substrate, a copper electrode layer formed by sputtering and arranged on the ceramic substrate, a protective layer at the outside of the ceramic substrate and a pin on a lower end of the protective layer, and the protective layer is stacked on the surface of the copper electrode layer; and the protective layer is formed by a nano alumina film. The preparation method comprises the following steps: (1) cleaning the ceramic substrate, and drying the same; (2) sputtering copper on the surface of the ceramic substrate in a magnetron sputtering manner to form the copper electrode layer; and (3) after the pin is connected to the copper electrode, sputtering a layer of nano aluminum film on the surface of the copper electrode layer in the magnetron sputtering manner, and performing sintering in a weak oxidizing atmosphere to formthe nano alumina film. According to the negative temperature coefficient thermistor disclosed by the invention, the copper is used as the electrode layer, the nano aluminum and copper form a solid solution alloy, thereby enhancing the bonding strength of the electrode layer and the nano aluminum, and the sintering is performed in the weak oxidizing atmosphere at a low vacuum degree, so that the surface of the nano aluminum is oxidized to form a layer of dense alumina film so as to function as a protective layer.
Owner:JIANGSU SHIRUI ELECTRONICS CO LTD

Quaternary positive electrode material and preparation method and application thereof

The invention relates to the technical field of lithium batteries, in particular to a quaternary positive electrode material and a preparation method and application thereof. The preparation method of the quaternary positive electrode material comprises the following steps of: mixing a positive electrode base material, soluble cobalt salt, a precipitator, a complexing agent and water, carrying out liquid-solid separation on a mixed system, collecting a solid phase, and drying and calcining the solid phase. The chemical general formula of the positive electrode base material is LiNi<x>Co<y>Mn Al <1-x-y-z>O2, wherein x is more than or equal to 0.9 and less than 1, y is more than 0 and less than 0.07, and z is more than 0 and less than 0.03. According to the invention, the positive electrode base material is washed and coated, and the surface of the positive electrode base material is uniformly coated with a layer of cobalt compound, so that the conductivity of the material is improved, the function of a protective layer is realized, and side reaction of the positive electrode material and electrolyte is reduced; and meanwhile, the precipitant can settle a residual lithium source, so that the situation that the capacity exertion and the circulation retention rate of the positive electrode material are affected due to the fact that the pH value is too high in the later homogenization process and a jelly-like condition occurs is prevented.
Owner:SVOLT ENERGY TECHNOLOGY CO LTD

Light emitting diode and preparation method thereof, and application thereof

The present invention discloses a light emitting diode and a preparation method thereof, and an application thereof. The light emitting diode comprises an anode, a cathode and a light emitting unit combined between the anode and the cathode, the anode comprises a first metallic oxide layer, a graphene layer and a second metallic oxide layer combined in a stack mode in order, and the first metallicoxide layer or the second metallic oxide layer and the light emitting unit are combined in a stack mode. The anode included in the light emitting diode is a composite sandwich construction to achieveimprovement of the light take-out efficiency of the light emitting diode to increase the photoelectric efficiency and the electrical stability and the mechanical stability of the anode and ensure thelife stability of the light emitting diode. The preparation method of the light emitting diode ensures the electrical stability and the mechanical stability of the prepared light emitting diode, andthe application of the light emitting diode comprises application in a display screen or solid state illumination lamps and lanterns.
Owner:TCL CORPORATION

Method for preventing clearance generation between different materials in semiconductor device

The invention discloses a gap avoiding method among semiconductors of different materials, which comprises the following steps: forming W layer with Ti-TiN-W stacked metal layer LM1 on the polysilicon touching piston; forming the second borophosphor silicate glass (BPSG) layer barrier layer on the polysilicon touching piston to prevent erosion LM1 layer with chlorine ion from eroding polysilicon touching piston. The invention can avoid gap between W layer storage unit contacts (CCT), when forming storage unit W layer contact.
Owner:SEMICON MFG INT (SHANGHAI) CORP +1

Composition for treating gummosis of trunk and main branches of lemon tree and preparation method and using method thereof

The invention belongs to technical field of disease and pest control of fruit trees, and discloses a composition for treating gummosis of a trunk and main branches of a lemon tree and a preparation method and a using method thereof. The composition for treating the gummosis of the trunk and the main branches of the lemon tree comprises the following components in parts by mass: 100-150 parts of quick lime, 10-15 parts of sulphur, 2-5 parts of agricultural streptomycin sulfate, 5-8 parts of metalaxyl-mancozeb, 5-10 parts of methyl thiophanate, 2-5 parts of table salt and 1000 parts of water. The composition can effectively treat the gummosis of the trunk and the main branches of the lemon tree; the composition is simple in components, convenient to use, labor-saving, time-saving and money-saving, can achieve an effective treatment effect on the gummosis of the trunk and the main branches of the lemon tree, has the curative rate of 80% or above, and does not damage the trunk body.
Owner:INST OF TROPICAL & SUBTROPICAL CASH CROP YUNNAN ACAD OF AGRI SCI +1

Photoelectrode and preparation method and application thereof

ActiveCN112251764AImproving the performance of photocatalytic hydrogen evolutionImproving the efficiency of hydrogen production by electrolysis of waterElectrodesPhysical chemistryThin membrane
The invention provides a photoelectrode and a preparation method and application thereof. The photoelectrode comprises a fluorine-doped stannic oxide glass carrier and a functional layer located on the fluorine-doped stannic oxide glass carrier, and the functional layer comprises a Mo2CTx film layer. The preparation method comprises the step of preparing a functional layer on a fluorine-doped stannic oxide glass carrier to obtain the photoelectrode, wherein the step of preparing the functional layer comprises the step of preparing a Mo2CTx thin film layer in a Mo2CTx solution through an impregnation method. The photoelectrode provided by the invention is good in stability, high in efficiency, simple in preparation process and low in cost when being used for photoelectric hydrogen evolution.
Owner:TIANJIN UNIV RENAI COLLEGE

Black wolfberry effervescent tablet and preparation method thereof

ActiveCN104783292BRetain bioactive ingredientsShorten extraction and separation timeFood scienceSodium bicarbonateEffervescent tablet
The invention belongs to the field of functional solid beverage processing, and particularly relates to a Lycium ruthenicum Murr effervescent tablet and a preparation method thereof. The Lycium ruthenicum Murr effervescent tablet comprises the following components: 45-50% of Lycium ruthenicum Murr extractive powder, 25-30% of organic acid, 18-23% of alkali, and 4-5% of PEG6000 or a mixture of PEG6000 and sodium chloride, wherein after the PEG6000 or the mixture of PEG6000 and sodium chloride coats the alkali, the raw materials are uniformly mixed and subjected to tabletting; the organic acid is citric acid or tartaric acid, and the alkali is sodium bicarbonate or sodium carbonate. According to the invention, a small amount of calcium carbonate added during the extraction process of Lycium ruthenicum Murr effective constituents can react with the citric acid in the extracting solution to generate calcium citrate, so that the stability of the Lycium ruthenicum Murr effective constituents is improved; besides, the method of melting at high temperature and coating at low temperature is adopted during the preparation of the PEG coated object, so that the stability of the effervescent tablet is improved.
Owner:SHENYANG PHARMA UNIVERSITY +1

Peanut harmless and environment-friendly planting method

The invention discloses a peanut harmless and environment-friendly planting method, and relates to the field of peanut planting. The planting method includes the steps: (1) land consolidation; (2) fertilization; (3) seed pretreatment; (4) sowing; (5) field management; (6) harvesting. The peanut planting method has standard operation processes, unified management is facilitated when peanuts are planted, and used organic fertilizers and insecticides are natural organic substances, green, environmentally friendly and free from toxic substances and residues when the peanuts are planted.
Owner:HEFEI HUIGU AGRI TECH CO LTD

A kind of negative temperature coefficient thermistor with nanoparticle film and its preparation method

The invention discloses a negative temperature coefficient thermistor with a nano particle film, and a preparation method thereof. The thermistor comprises a ceramic substrate, a copper electrode layer formed by sputtering and arranged on the ceramic substrate, a protective layer at the outside of the ceramic substrate and a pin on a lower end of the protective layer, and the protective layer is stacked on the surface of the copper electrode layer; and the protective layer is formed by a nano alumina film. The preparation method comprises the following steps: (1) cleaning the ceramic substrate, and drying the same; (2) sputtering copper on the surface of the ceramic substrate in a magnetron sputtering manner to form the copper electrode layer; and (3) after the pin is connected to the copper electrode, sputtering a layer of nano aluminum film on the surface of the copper electrode layer in the magnetron sputtering manner, and performing sintering in a weak oxidizing atmosphere to formthe nano alumina film. According to the negative temperature coefficient thermistor disclosed by the invention, the copper is used as the electrode layer, the nano aluminum and copper form a solid solution alloy, thereby enhancing the bonding strength of the electrode layer and the nano aluminum, and the sintering is performed in the weak oxidizing atmosphere at a low vacuum degree, so that the surface of the nano aluminum is oxidized to form a layer of dense alumina film so as to function as a protective layer.
Owner:JIANGSU SHIRUI ELECTRONICS CO LTD

A kind of quaternary cathode material and its preparation method and application

The invention relates to the technical field of lithium batteries, in particular to a quaternary positive electrode material and a preparation method and application thereof. The preparation method of the quaternary positive electrode material comprises the following steps: mixing the positive electrode substrate, the soluble cobalt salt, the precipitating agent, the complexing agent and water, separating the liquid from the solid in the mixed system, collecting the solid phase, drying and calcining; The general chemical formula of the positive electrode substrate is LiNi x co y mn z al (1‑x‑y‑z) o 2 , wherein, 0.9≤x<1, 0<y<0.07, 0<z<0.03. In the present invention, the positive electrode base material is washed and coated, and a layer of cobalt compound is evenly coated on the surface to improve the conductivity of the material and play the role of a protective layer, reducing the occurrence of side reactions between the positive electrode material and the electrolyte; at the same time, the precipitation The agent can settle down the remaining lithium source, prevent the jelly-like situation due to the high pH in the later homogenization process, and affect the capacity of the positive electrode material and the cycle retention rate.
Owner:SVOLT ENERGY TECHNOLOGY CO LTD

Anti-impact and abrasion hydraulic surface protection block body and preparation method thereof

The invention discloses an anti-impact and abrasion hydraulic surface protection block body and a preparation method thereof. The hydraulic surface protection block body comprises a concrete block body and a geopolymer layer covering the surface of the concrete block body, and an adhesive is arranged between the geopolymer layer and the concrete block body. The geopolymer layer is formed by bonding a geopolymer on the concrete block body of the hydraulic surface protection block body by an adhesive through a form of coating. According to the anti-impact and abrasion hydraulic surface protection block body, the anti-wear property of the surface protecting block body is good, and erosion of a water flow to the block body is effectively reduced; the coating is used for replacing a common concrete surface, so that strength of the surface layer of the surface protection block body can be enhanced, and the service life of the surface protection block body is prolonged; the coating material is prepared from a plurality of industrial wastes, so that cost for additionally arranging block protection layers is saved; and the preparation method is simple and is high in safety.
Owner:HOHAI UNIV

Method for activating dopant atoms

The invention provides a method for activating dopant atoms, which includes steps of making a polycrystalline silicon grid on a silicon base of a silicon chip, performing low-concentration ion implantation of boron atoms to the upper surface of the silicon chip to form a P+ type silicon substrate on the silicon base on two sides of the polycrystalline silicon grid, making side walls consisting of silicon nitride on two sides of the polycrystalline silicon grid, performing source electrode and drain electrode ion implantation on the P+ type silicon substrate on the outer sides of the side walls on two sides of the polycrystalline silicon grid to form a source electrode and a drain electrode, processing the upper surface of the silicon chip by plasmas of nitrogen-enriched gas to form saturated and stable silicon-nitrogen bond compound on bare surfaces of the source electrode, the drain electrode, the outer sides of the side walls and the top of the polycrystalline silicon grid, depositing silicified retaining layers on the bare surfaces of the source electrode, the drain electrode, the outer sides of the side walls and the top of the polycrystalline silicon grid of the silicon chip, and annealing the silicon chip. The method can effectively increase proportion of activated boron atoms.
Owner:SEMICON MFG INT (SHANGHAI) CORP +1

Buckling restrained brace large cantilever structure system

The invention relates to a buckling restrained brace large cantilever structure system, and belongs to the field of structural engineering. The structure system comprises a concrete combined column, aspecial-shaped cantilever beam and a buckling restrained brace arranged between the concrete combined column and the special-shaped cantilever beam; and the concrete combined column and the bucklingrestrained brace, and the special-shaped cantilever beam and the buckling restrained brace are connected through assembled metal connecting pieces. The buckling restrained brace is adopted as a support member of the structure system, and the special-shaped cantilever beam is connected with a square-section combined column; and the whole anti-seismic performance of the large cantilever structure system is effectively improved by utilizing the good energy dissipation and shock absorption effects of the buckling restrained brace. All parts of components in the whole structure system are connectedin an all-bolt connection mode, the field welding work is reduced, the connection is safe and reliable, the field assembly degree is high, the construction speed is high, the construction quality iseasy to guarantee, and the comprehensive cost performance is greatly improved.
Owner:THE THIRD CONSTR OF CHINA CONSTR EIGHTH ENG BUREAU

Perovskite material, solar cell device and preparation method

The invention relates to a perovskite material, a solar cell device and a preparation method. The perovskite material is prepared by cross-linking a main body material and a hydrophobic micromolecular cross-linking agent, wherein the main body material is organic-inorganic perovskite, and the hydrophobic micromolecular cross-linking agent is a trifluoropropyl bis(aziridine) compound. The perovskite material is uniform and compact in film formation and has excellent water vapor stability, the solar cell device is high in operation stability, and the preparation methods of the perovskite material and the solar cell device are simple and feasible.
Owner:中山复元新材料科技有限责任公司

Manufacturing method for automobile sign capable of realizing radar active cruise function

The invention discloses a manufacturing method for an automobile sign capable of realizing a radar active cruise function. The manufacturing method includes obtaining a transparent plastic sign by means of manufacturing transparent polycarbonate plastics into a cambered panel with the thickness ranging from 5mm to 6mm by the aid of an injection molding machine; obtaining a transparent plastic sign with a metallic impression and a protecting layer by the aid of UV (ultraviolet) spraying materials; obtaining an indium-plated plastic sign by means of forming an indium metal film on the transparent plastic sign by an ion sputtering method; forming a seamless automobile sign finished product with uniform wall thickness ranging from 7cm to 8cm by means of assembling the indium-plated plastic sign with a plastic base; and finally detecting attenuation and obtaining the qualified automobile sign with the attenuation lower than 2.0DB (decibel) and the capability of realizing the radar active cruise function. The automobile sign has the advantages that the automobile sign has metallic luster, is penetrable by radar microwaves, can realize a function of radar measurement for front and rear automobile distances and the active cruise function, has an appearance decoration function and can meet safety requirements.
Owner:NINGBO SWELL IND CO LTD

A high solid content semi-solid electrode, its preparation method and a lithium slurry flow battery comprising the electrode

The invention relates to the composition and preparation method of a semi-solid electrode with high solid content and a lithium slurry flow battery thereof. The electrode is composed of an active material, a conductive agent, a nanostructured dispersant grafted with a polar alkyl chain on the surface, an electrolyte composition, and a current collector. It is characterized in that the mass ratio of the active material particles in the semi-solid electrode can be up to 90% of the slurry system, and the dispersant and conductive agent are jointly adsorbed on the surface of the active material particles, on the one hand, it reduces the direct contact between the active material and the electrolyte, reduces the possibility of side reactions, and improves the coulombic efficiency of the battery; On the other hand, the nanostructured dispersant effectively reduces the viscosity and shear strength of the semi-solid electrode, so the electrode has better fluidity under the same solid content, which is beneficial to reduce the power consumption during the flow process of the slurry flow battery electrode . The electrode is used in a lithium slurry flow battery, which can improve the first Coulombic efficiency and energy density of the battery, and is conducive to the long-term and stable cycle of the battery.
Owner:INST OF PROCESS ENG CHINESE ACAD OF SCI +1

Banana interplanting method suitable for Zhangzhou regions

The invention provides a banana interplanting method suitable for Zhangzhou regions. The method includes: selecting a local variety 'Tianbaoaijiao' of Zhangzhou and a disease-resistant variety 'Nantianhuang' for interplanting; in May of each year, transplanting Tianbaoaijiao greenhouse cultivation cupped seedlings into a field, planting Niantianhuang seedlings at 1 / 3 positions of an interval between each two plants of Tianbaoaijiao in February of the next year, lopping 1 / 2 of stalks of Tianbaoaijiao after being harvested, harvesting Nantianhuang mature fruits from March to April of the next year, and cyclically planting. By the method, damage caused by typhoon and frost can be avoided effectively, different banana varieties can be rotated, varieties with long development period can realizeone time of harvesting in a year, economic income of banana growers can be increased, and utilization rate and yield of land can be increased.
Owner:FUJIAN ACADEMY OF AGRI SCI SUBTROPICAL AGRI RES INST

Method for culturing and observing plaque

ActiveCN113667647AAdd pre-check linkGuarantee the success of the experimentBacteriaMicroorganism based processesBiotechnologyEscherichia coli
The invention relates to a method for culturing and observing plaque. The method takes escherichia coli as a host, separates bacteriophage and cultures the plaque, and comprises the following steps: pre-detecting a water sample, preparing a bacterial suspension, treating the water sample and proliferating the bacteriophage, diluting a bacteriophage proliferating solution, preparing a bacteriophage-host mixed solution, culturing the plaque by a sandwich plate method, conducting dyeing and observing and the like, so as to finally observe clear plaque. Escherichia coli moss is in a light blue cloud shape, and plaques are dark blue transparent small circular plaques one by one. Compared with a traditional double-layer plate method, the method has the advantages that the operation difficulty of the experiment is remarkably reduced, the risk of polluting other environmental microorganisms is reduced, the success rate of the experiment is improved, and the method is a good teaching experiment method and can be applied to college teaching or related technician training. The sandwich plate method disclosed by the invention can also be used for culturing and observing plaque formed by other bacteriophages, but related steps need to be adjusted.
Owner:BAODING UNIV
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