The invention relates to a rubber glove leak detection device. The rubber glove leak detection device comprises a water tank filled with a liquid B; a lifting mechanism is arranged outside the water tank; a fixed joint fixed on the lifting mechanism; the fixed joint is provided with a gas conduction pipe, a liquid conduction pipe and a vacuum pipe; the gas conduction pipe, the liquid conduction pipe and the vacuum pipe are provided with a gas inlet valve, a water inlet valve and a vacuum valve respectively; the gas inlet valve, the water inlet valve and the vacuum valve are connected with an air compressor, a liquid storage tank and a vacuum pump respectively; the gas conduction pipe and the vacuum pipe are provided with a pressure sensor and a vacuum sensor respectively; the liquid storage tank is filled with a liquid A; the fixed joint is sleeved with a holding hoop; a sensor probe is arranged in the water tank; a chromaticity detector is installed on a controller; and the gas inlet valve, the air compressor, the pressure sensor, the water inlet valve, the vacuum valve, the vacuum pump, the vacuum sensor, the lifting motor and the chromaticity detector are electrically connected with the controller. The invention also discloses a detection method. When a glove has a loophole, the two liquids meet, the colors of the liquids change, and the chromaticity detector emits an alarm, and therefore, rubber products can be detected quickly and accurately.