The invention relates to a high-efficiency bio gel sealant and application thereof, which consists of main gel lyophilized powder, catalyst lyophilized powder, main gel cytolysate and catalyst cytolysate; during use, the main gel lyophilized powder is dissolved in the main gel cytolysate and the catalyst lyophilized powder is dissolved in the catalyst cytolysate, which are respectively absorbed in an aseptic injector of a double-cavity liquid pusher; the two solution are evenly mixed by the conehead of the double-cavity liquid pusher, and then sprayed on the wounds after surgery and bleeding, so as to form a semi-transparent ivory film; the invention also discloses a method of preparing all components of the high-efficiency bio gel sealant, including preparation of the main gel lyophilized powder, the catalyst lyophilized powder, etc. with pig blood as the raw material; the product of the invention is an absorbable bio gel combination which can be used immediately for blood stopping, sealing and adhesion of tissues, and is widely applicable in surgery fields, such as general surgery, orthopedics, cardiothoracic surgery, neurosurgery, obstetrics and gynaecology, etc.