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Preparation method for chitosan-based biomimetic membrane material with asymmetric structure

An asymmetric structure, chitosan technology, used in medical science, surgery and other directions, can solve the problems of fast degradation, fin shedding, allergic reactions, etc., achieve controllable degradation, good bone induction regeneration performance, and simple preparation process Effect

Inactive Publication Date: 2015-08-19
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Bio-Gide membranes will have complications such as flap loss, bleeding, local inflammation, bone loss, infection or pain, and allergic reactions, and the degradation rate is too fast

Method used

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  • Preparation method for chitosan-based biomimetic membrane material with asymmetric structure
  • Preparation method for chitosan-based biomimetic membrane material with asymmetric structure

Examples

Experimental program
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Effect test

Embodiment 1

[0017] 1) Disperse chitosan powder in 2% acetic acid aqueous solution, stir until completely dissolved, and prepare 2 wt % chitosan solution, then add anhydrous methanol and acetic anhydride to it to make anhydrous methanol The volume ratio with water is 4:3, the mass ratio of chitosan and acetic anhydride is 3.5, after stirring evenly, let it stand for reaction for 1.5h to obtain an acid-soluble chitosan solution;

[0018] 2) Centrifuge the acid-soluble chitosan solution in step 1) at a speed of 5000rpm for 10min, remove the precipitate, drop into it a NaOH aqueous solution with a mass fraction of 5% under stirring conditions until the solution is alkaline, filter and use three Wash the filtrate with distilled water until the washing liquid is neutral to obtain chitosan microgel;

[0019] 3) Add the chitosan microgel in step 2) into the glycerol aqueous solution with a volume fraction of 10%, stir for 30 minutes to obtain a mixed solution, inject the mixed solution into a mol...

Embodiment 2

[0023] 1) Disperse chitosan powder in 2% acetic acid aqueous solution, stir until completely dissolved, and prepare 2.5 wt % chitosan solution, then add anhydrous methanol and acetic anhydride to it to make anhydrous methanol The volume ratio with water is 5:3, the mass ratio of chitosan and acetic anhydride is 3.8, after stirring evenly, let stand for reaction 2h, obtain acid-soluble chitosan solution;

[0024] 2) Centrifuge the acid-soluble chitosan solution in step 1) at a speed of 6000rpm for 12min, remove the precipitate, drop into it a KOH aqueous solution with a mass fraction of 5% under stirring conditions until the solution is alkaline, filter and use three Wash the filtrate with distilled water until the washing liquid is neutral to obtain chitosan microgel;

[0025] 3) Add the chitosan microgel in step 2) into the glycerol aqueous solution with a volume fraction of 12%, stir for 30 minutes to obtain a mixed solution, inject the mixed solution into a mold, and let it...

Embodiment 3

[0029] 1) Disperse chitosan powder in 2% acetic acid aqueous solution, stir until completely dissolved, and prepare 3 wt % chitosan solution, then add anhydrous methanol and acetic anhydride to it to make anhydrous methanol The volume ratio with water is 4:3, the mass ratio of chitosan and acetic anhydride is 4.0, after stirring evenly, let it stand for reaction for 2h to obtain an acid-soluble chitosan solution;

[0030] 2) Centrifuge the acid-soluble chitosan solution in step 1) at a speed of 6500rpm for 15min, remove the precipitate, drop into it a KOH aqueous solution with a mass fraction of 5% under stirring conditions until the solution is alkaline, filter and use three Wash the filtrate with distilled water until the washing liquid is neutral to obtain chitosan microgel;

[0031] 3) Add the chitosan microgel in step 2) into the glycerol aqueous solution with a volume fraction of 15%, stir for 35 minutes to obtain a mixed solution, inject the mixed solution into a mold, ...

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Abstract

The invention discloses a preparation method for a chitosan-based biomimetic membrane material with an asymmetric structure, and the preparation method comprises the following steps of dispersing chitosan into acetic acid aqueous solution, adding absolute methanol and acetic anhydride for reacting after the chitosan is completely dissolved, and then, centrifugally removing sediment; dropping in alkaline solidification liquid under stirring condition, and filtering to obtain chitosan microgel; adding the chitosan microgel in glycerin solution, injecting the solution in a mold, and standing and draining water, freeze-drying one part to obtain a chitosan-based porous sponge material, and drying the other part to obtain a chitosan-based dense membrane material; bonding the chitosan-based porous sponge material and the chitosan-based dense membrane material to obtain the chitosan-based biomimetic membrane material with asymmetric structure. The preparation method provided by the invention has the advantages that the preparation technology is simple, the prepared chitosan-based biomimetic membrane material has a porous sponge structure and a dense membrane material structure, the chitosan-based biomimetic membrane material is not curly when encountering water, guiding bone regeneration performance is good, and the chitosan-based biomimetic membrane material is degradable and controllable and can be widely used as a guided bone tissue regeneration membrane material in treatment of the fields such as dental implant, osteoarthritis, bone defect, and the like.

Description

technical field [0001] The invention relates to a preparation method of a chitosan-based biomimetic membrane material with an asymmetric structure. Background technique [0002] Chitosan is a biodegradable natural polysaccharide, its degradation product is glucosamine, which is weakly alkaline, non-toxic and harmless to the human body and tissues, has excellent biocompatibility, natural drug activity, anti-tumor Activity, anti-inflammatory effect, etc. Chitosan has been widely used in tissue engineering, drug release, postoperative anti-adhesion film, orthopedic repair materials and other fields. [0003] Dental implants have gradually emerged and become popular in the field of dentistry. Periodontal Guided Tissue Regeneration (GTR) is the most advanced periodontal surgical treatment method today, that is, a barrier membrane material is placed on the root and gum tissue Between the flaps, the gingival connective tissue cells and epithelial cells are physically prevented fr...

Claims

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Application Information

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IPC IPC(8): A61L31/04A61L31/14
Inventor 王征科杨玲陆文涛胡巧玲
Owner ZHEJIANG UNIV
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