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High-efficiency biogum sealant and uses thereof

A technology of biological glue and sealing agent, applied in the field of biomedicine, can solve the problems of high product cost, high price, easy to bring safety, etc., achieve high reliability and safety, simple preparation process, and avoid the effect of spreading opportunities

Active Publication Date: 2008-07-09
GUANGZHOU BIOSEAL BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Chinese patent CN1246047C discloses a suspension containing fibrinogen, thrombin and alcohol and a method for coating the carrier thereof. The fibrinogen and thrombin in this patent are human or bovine fibrinogen and thrombin. The fibrin of the protein sealant is also mostly derived from human blood, and most of the thrombin is extracted from human blood, and some of them use cow blood as the raw material; using human blood as the raw material for the preparation of the fibrin sealant has the potential to spread HBV, HIV and other viruses Dangerous, while cow blood products have the possibility of spreading mad cow disease and infecting other zoonotic diseases, and can cause human coagulation factor V deficiency; in addition, the use of human blood as raw materials is likely to lead to high product costs and high prices, which are widely used in developing countries Applications are subject to certain limitations
Although this fibrinogen-thrombin dressing does not require premixing and is convenient to use, its application is also limited by its need to be stored at 4°C and pre-wetted with saline solution prior to application to the wound. restricted
Chinese patent CN1214446C discloses a highly coagulable fibrinogen gum, the fibrinogen is human fibrinogen, bovine fibrinogen or porcine fibrinogen, and human blood or bovine blood is also used in the raw material; and in Organic solvents such as acetone and ether are used in the production process, which not only increases the production cost, but also easily brings safety hazards during production

Method used

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  • High-efficiency biogum sealant and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 main body gel freeze-dried powder preparation one

[0031] Collect 55000ml of pig blood, add 6000ml of anticoagulant containing 0.147mol / L sodium citrate and 0.154mol / L sodium chloride, let it stand in a low-temperature storage at 4°C for 6 hours, let it separate into layers, and absorb the upper layer by siphon method Plasma, the lower layer of red blood cells was discarded; the upper layer of plasma was centrifuged in a high-speed refrigerated centrifuge at 10°C at a speed of 3200 rpm for 40 minutes, the plasma was collected, and filtered with a 0.22 μm filter membrane; 20,000ml of filtered plasma was taken, and 73.5g of glycine was added , to make it dissolve, cool the plasma to 4°C, add 2605.4g of sodium acetate to dissolve it, stir for 30 minutes, centrifuge at 3500 rpm for 40 minutes in a high-speed refrigerated centrifuge at 10°C, collect the precipitate, and obtain 2520.8g of precipitate . Crush the collected precipitate, add 33 liters of a buffer ...

Embodiment 2

[0032] Embodiment 2 main body gel freeze-dried powder preparation two

[0033] Collect 50000ml of pig blood, add 5700ml of anticoagulant containing 0.151mol / L sodium citrate and 0.148mol / L sodium chloride, let it stand in a low-temperature storage at 4°C for 8 hours, let it separate into layers, and absorb the upper layer by siphon method Plasma, the lower layer of red blood cells was discarded; the upper layer of plasma was centrifuged in a high-speed refrigerated centrifuge at 10°C at a speed of 5,000 rpm for 30 minutes, the plasma was collected, and filtered with a 0.22 μm filter membrane; 18,000ml of filtered plasma was taken, and 86.5g of glycine was added , to make it dissolve, cool the plasma down to 2°C, add 3024.8g of sodium acetate to dissolve, stir for 30 minutes, centrifuge at 4000 rpm for 30 minutes in a high-speed refrigerated centrifuge at 10°C, collect the precipitate, and obtain 2490.8g of precipitate . Crush the collected precipitate, add 32 liters of a buff...

Embodiment 3

[0034] Embodiment 3 main body gel freeze-dried powder preparation three

[0035]Collect 60000ml of pig blood, add 6000ml of anticoagulant containing 0.145mol / L sodium citrate and 0.151mol / L sodium chloride, let it stand in a low-temperature storage at 5°C for 6 hours, let it separate into layers, and absorb the upper layer by siphon method Plasma, the lower layer of red blood cells was discarded; the upper layer of plasma was centrifuged at 5,000 rpm at 10°C for 60 minutes in a high-speed refrigerated centrifuge, the plasma was collected, and filtered with a 0.22 μm filter membrane; 22,000ml of filtered plasma was taken, and 95.8g of glycine was added , to make it dissolve, cool the plasma down to 3°C, add 2931.8g of sodium acetate to dissolve it, stir for 30 minutes, centrifuge at 6000 rpm for 30 minutes in a high-speed refrigerated centrifuge at 10°C, collect the precipitate, and obtain 2685.8g of precipitate . Crush the collected precipitate, add 35 liters of a buffer solu...

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PUM

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Abstract

The invention relates to a high-efficiency bio gel sealant and application thereof, which consists of main gel lyophilized powder, catalyst lyophilized powder, main gel cytolysate and catalyst cytolysate; during use, the main gel lyophilized powder is dissolved in the main gel cytolysate and the catalyst lyophilized powder is dissolved in the catalyst cytolysate, which are respectively absorbed in an aseptic injector of a double-cavity liquid pusher; the two solution are evenly mixed by the conehead of the double-cavity liquid pusher, and then sprayed on the wounds after surgery and bleeding, so as to form a semi-transparent ivory film; the invention also discloses a method of preparing all components of the high-efficiency bio gel sealant, including preparation of the main gel lyophilized powder, the catalyst lyophilized powder, etc. with pig blood as the raw material; the product of the invention is an absorbable bio gel combination which can be used immediately for blood stopping, sealing and adhesion of tissues, and is widely applicable in surgery fields, such as general surgery, orthopedics, cardiothoracic surgery, neurosurgery, obstetrics and gynaecology, etc.

Description

technical field [0001] The invention relates to a high-efficiency bio-glue sealant, which belongs to the field of biomedicine; specifically, it relates to a bio-glue pharmaceutical composition that can quickly form a high-efficiency bio-glue film and has a high degree of hemostasis, adhesion and wound sealing. A biological hemostatic material. Background technique [0002] Controlling bleeding (bleeding) is a critical aspect of surgery, first aid, and field trauma care. Unfortunately, bleeding (bleeding) from various causes is very common, accidental injury bleeding (bleeding) in daily life and work, traumatic bleeding (bleeding) in surgery, traumatic bleeding (bleeding) from war, etc., If it cannot be controlled and handled in a timely manner, it may cause huge losses or even death. Facts have shown that through the use of simple and effective bleeding control methods, great convenience can be achieved in surgery, first aid and field trauma care. [0003] Fibrinogen and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/10A61L15/32A61L33/12
Inventor 陈彥文李吉来肖尚志黄星江彩霞潘小宁
Owner GUANGZHOU BIOSEAL BIOTECH
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