92 results about "TREHALOSE DIHYDRATE" patented technology

The present invention relates to a highly concentrated, stable pharmaceutical formulation of a pharmaceutically active anti-HER2 antibody, such as e.g. Trastuzumab (HERCEPTIN™), Pertuzumab or T-DM1, or a mixture of such antibody molecules for subcutaneous injection. In particular, the present invention relates to formulations comprising, in addition to a suitable amount of the anti-HER2 antibody, an effective amount of at least one hyaluronidase enzyme as a combined formulation or for use in form of a co-formulation. The formulations comprise additionally at least one buffering agent, such as e.g. a histidine buffer, a stabilizer or a mixture of two or more stabilizers (e.g. a saccharide, such as e.g. α,α-trehalose dihydrate or sucrose, and optionally methionine as a second stabilizer), a nonionic surfactant and an effective amount of at least one hyaluronidase enzyme. Methods for preparing such formulations and their uses thereof are also provided.

The invention particularly discloses a composition for promoting opening of skin aquaporin, which is mainly proportionally prepared from deionized water, glycerol, carbomer, xanthan gum, lycine, trehalose, inositol, taurine, lauryl mammary gland sodium lactate, monomethyl-monosilane triol, oxyproline, aspartic acid, yeast amino acid, polyglutamic acid, sodium hyaluronate, dipotassium glycyrrhetate, purslane extracting solution, licorice extract, ceramide, phytosphingosine, cholesterol, oligopeptide-1, polyquaternary amine salt-14 and the like. The composition has the function of promoting opening of skin aquaporin, has better water replenishing and locking effect, and effectively prevents and resists the phenomena of dry skin mucosa and skin aging.

The invention discloses a method of increasing the extraction yield of trehalose, which belongs to sugar industry technical field. Adopting the techniques of hydrogenation and chromatographic resolution, the microorganism or enzyme are used for transforming the starch and the trehalose obtained undergoes the hydrogenation reaction with the mixing solution of the maltose, glucose and maltotriose; therefore the impurity in the mixing solution produces the maltitol, sorbierite and maltotriitol; the trehalose has no reducibility, and is stable in the hydrogenation reaction; and the trehalose is separated from the maltitol, sorbierite and maltotriitol in the mixing solution by the chromatographic resolution technique simulating the moving bed; and the purity and extraction yield of the trehalose are largely increased; the trehalose is crystallized by cooling, and the trehalose product is finally obtained. Compared with the technique of producing the trehalose by adopting the original microorganism or enzyme method, the technique of the invention increases the extraction yield over 80 percent, the sub product maltitol has a comparatively high utilization value; and therefore the production cost for producing the trehalose and the market price are reduced, which makes sense for increasing the market competition of trehalose.

The invention relates to a freezing liquid for human stem cells and a freezing storage method. Every 1000 ml of the freezing liquid comprises the following components: 20-50 mg of salidroside, 50-100 g of hydroxyethyl modified starch 450 Kd, 10-50 g of carboxylated poly-L-lysine, 50-100 g of sucrose, 20-150 g of trehalose, 5-20 g of antifreeze protein, 5-20 g of glycoprotein, 20-50 ml of DMSO, 60-100 ml of glycerin, and 200-300 ml of fetal calf serum, with the balance being a buffer solution. The freezing liquid for human stem cells is low in dimethyl sulfoxide content, has no side or toxic effect on frozen cells, can effectively keep the activity of stem cells, prevents oxidative injury, and is high in cell thawing rate.

The invention provides preserving fluid of hepatic cells for a biological artificial liver and a preparation method thereof. The preserving fluid is a solution compounded by ultrapure water. The solution contains the following components within the concentration range: 15-25mmol / L of disodium hydrogen phosphate, 1-10mmol / L of sodium hydrogen phosphate dehydrate, 4-6mmol / L of potassium citrate monohydrate, 10-30mmol / L of sodium chloride, 5-10mmol / L of magnesium chloride hexahydrate, 3-10mmol / L of disodium adenosine triphosphate, 1-5mmol / L of reducing glutathione, 0.1-0.5mmol / L of alpha-lipoic acid, 100-150mmol / L of trehalose (C6H12O5), 200 / 0.510-50g / L of hydroxyethyl starch and 2-10mg / L of matrine. The preparation method of the preserving fluid comprises the following steps of: accurately weighing all components according to the concentration requirements of the components, wherein the alpha-lipoic acid is weighed in a dark place; completely dissolving the other components except the alpha-lipoic acid by using the right amount of ultrapure water; sufficiently dissolving the alpha-lipoic acid in the dark place; and adding the ultrapure water to full dose. The preserving fluid can well protect the cell activity of the hepatic cells for the biological artificial liver and the special functions of the hepatic cells at low temperature so as to satisfy the short-term low temperature preservation of a large-scale hepatic cell bank for the biological artificial liver and / or the hepatic cell protection in the long-distance transportation process.

The invention relates to an extracellular matrix freeze-drying protection liquid. The extracellular matrix freeze-drying protection liquid takes normal saline as a solvent and comprises the following components: chondroitin sulfate, vitamin C, polyethylene glycol, trehalose, glucan, serine and threonine. The invention has the following advantages: the protection liquid has high penetrability, can be vitrified under a low-temperature condition, is rich in negative polar groups and hydroxyl groups, and can be efficiently bonded with collagen molecules; the stable and uniform extracellular matrix freeze-drying effect can be ensured; the protection liquid can be formed into the vitreous state at low temperature, thereby preventing ice crystals from damaging the protein structure; and a protection film is formed at the outer layer of the matrix in stead of hydrogen bonds formed by water molecules and collagen, thereby protecting the collagen function and the stability of the three-dimensional structure. The extracellular matrix freeze-drying protection liquid has low cytotoxicity, and can be used immediately after being rinsed with normal saline or sterilized water; the freeze-dried extracellular matrix can be preserved at normal temperature for more than three years when being packaged in vacuum; and the extracellular matrix freeze-drying protection liquid is suitable for the preservation of purified extracellular matrix such as collagen, elastin, glucoprotein and the like, and is also suitable for various biologic materials constructed by extracellular matrix materials.

The invention discloses a cell preservation solution. The cell preservation solution is a solution prepared from ultra pure water. The solution is a paraform preservation solution. The solution also comprises 0.4-0.8wt% of glutaraldehyde, 0.05-0.1mol / L potassium citrate, 0.035-0.15mol / L sodium diacetate, 0.1-0.3mol / L magnesium chloride hexahydrate, 0.08-0.4mol / L sodium chloride, 0.003-0.005mol / L alpha-lipoic acid, 0.08-0.12mol / L trehalose and 3-5mg / L matrine. The pH value of the cell preservation solution is 7.1-7.4, and the osmotic pressure of the cell preservation solution is 290-315mmol / L. According to the preservation solution disclosed by the invention, the cell preservation time is greatly prolonged, besides, the preparation method is simple, and the application range is wide.

The invention relates to an extraction, separation and purification method of trehalose, in particular to a separation and purification method of trehalose by one-step. The method comprises the steps of: after extracting dry yeast by using water, adding a flocculating agent to adjust the pH value; and then filtering to obtain clear filtrate, and directly concentrating and crystallizing to obtain trehalose crystals. The method has simple separation and purification steps, convenience and feasibility, low cost and good effect, and provides conditions for the trehalose to be widely used in the fields of medicine, cosmetics, food preservation and the like.

Provided is a production method whereby it is possible to produce a powder containing crystalline trehalose dihydrate with starch as a starting material with high powder-to-starch yields. More specifically, provided is a production method for a powder containing crystalline alpha, alpha-trehalose dihydrate and having at least 98.0mass% of alpha, alpha-trehalose on an anhydrous basis, said method including: a step in which liquefied starch is subjected to the actions of a starch debranching enzyme, cyclomaltodextrin glucanotransferase, an alpha-glycosyl trehalose producing enzyme derived from a micro-organism belonging to the genus arthrobacter, and a trehalose releasing enzyme derived from a micro-organism belonging to the genus arthrobacter, and then to the action of glucoamylase, to obtain an alpha, alpha-trehalose-containing saccharide solution; a step in which alpha, alpha-trehalose dihydrate is crystallized from the saccharide solution; and a step in which the crystallized alpha, alpha-trehalose dihydrate is extracted by centrifugation, aged, and dried. The production method is characterized in that a natural or recombinant enzyme derived from a micro-organism belonging to the genus paenibacillus or a variant of such an enzyme is used as the cyclomaltodextrin glucanotransferase such that the content of alpha, alpha-trehalose in the saccharide solution is over 86.0mass% on an anhydrous basis, without having to undergo a column chromatography fractionation step.

The invention discloses a detecting reagent of urea nitrogen and detecting test paper of urea nitrogen. The detecting reagent contains 50-64 KU / L urease, 1.0-1.5 g / L trehalose and 20-30 g / L developing matter. According to the detecting reagent of urea nitrogen, multiple specific enzymes are specifically matched, and the aim of fast and accurately detecting the content of urea nitrogen can be achieved. The detecting test paper of urea nitrogen comprises a reaction base layer, a reaction layer, a flood filtering layer and a sample suction layer. The layers are overlapped to form a siphon system, the anti-interference capacity is higher, interference of endogenous substances is effectively eliminated, and it is further guaranteed that the content of urea nitrogen is fast and accurately detected.

The invention encompasses a powder composition with low hygroscopicity, excellent storage stability, easy uniform dispersion of a core substance, a sufficient amount of the core substance, and excellent taste without attachment onto teeth even after addition to chewing gum or tablet confectioneries, which is preferable for use as an agent for giving aroma and flavor or an agent for enhancing aroma and flavor, as well as foods and drinks containing the same. The powder composition is produced by melting a carbohydrate mixture comprising about 30% to 70% by mass of trehalose and a plasticizer under heating, adding a core substance to the resulting molten matter for mixing to obtain a uniform mixture, extruding the uniform mixture while cooling the uniform mixture, for solidification, and cutting or grinding the resulting solid matter. The resulting powder composition is kneaded with for example gum base, powder sugar, corn syrup and the like and is then spread into stick chewing gum.

The invention discloses quick luminescent bacteria supported based on signal molecule. The quick luminescent bacteria supported based on signal molecule are characterized in that: luminescent bacteria freeze-dried powder is prepared by mixing bacterial mud obtained by centrifuging 10ml of Vibrio fischeri at the concentration of 10<9> / ml and 1ml of cryoprotective agent, and performing freeze drying, and is stored at -20DEG C for 6 months; and the cryoprotective agent is prepared by dissolving 15g of nonfat milk, 5g of glycerol and 12.5g of trehalose in 100ml of signal molecule solution. The invention has the advantages that: by adding signal molecules, the synthesis of luciferase can be promoted, so that the time for the luminescent bacteria freeze-dried powder to restore stable luminescence is shortened from the original 15-30 minutes to 5 minutes; and by adding the composite cryoprotective agent, more bacteria can keep the integrity of cellular structures and the activity of cells during freezing and drying, and can be revived under proper conditions.

The invention relates to a cryoprotectant based on liquid metal nanoparticles as well as a method and an application of the cryoprotectant. The cryoprotectant comprises the liquid metal nanoparticlesand a basic protectant, wherein the melting point of the raw material of the liquid metal nanoparticles is below 60 DEG C, and the basic protective agent is a mixture of ethylene glycol, propylene glycol and trehalose. The cryoprotectant can realize vitrification in the cooling process, and can be used as a space heat source under the irradiation of near-infrared laser in the rewarming process, thereby greatly enhancing the heating rate, inhibiting anti-vitrification, reducing the formation of ice crystals, and enhancing the survival rate and structural integrity of cryopreserved cells and vascular tissues.

The invention discloses a liquid organic fertilizer for reducing heavy metal pollution of soil and a production method of the liquid organic fertilizer. The liquid organic fertilizer is prepared fromthe following components in parts by weight: 40-50 parts of animal viscera, 20-30 parts of chicken manure, 30-40 parts of pig manure, 20-30 parts of grape skins, 20-30 parts of coconut shell powder, 20-30 parts of shrimp and crab shell powder, 5-10 parts of a microbial agent, 5-10 parts of amino acid, 25-35 parts of charcoal, 4-8 parts of soybean lecithin, 5-10 parts of succinyl trehalose lipid, 5-10 parts of disaccharide single-lipid rhamnolipid and 2000-3500 parts of water. The liquid organic fertilizer disclosed by the invention has high affinity for various heavy metal ions, so that the content of heavy metals in the soil can be reduced, and thus accumulation of the heavy metals in the plants can be reduced. Meanwhile deficiency of beneficial trace elements cannot be caused, adsorptionforce is strong and durable, activation or desorption is not easy, and in-situ remediation on soil heavy metals is realized while nutrients are provided for crops.

A method of lyophilizing exosomes using a cryoprotectant comprising methionine, mannitol and trehalose is disclosed. The lyophilized exosome product shows a good appearance which maintains a porous sponge shape without forming ice crystals. In addition, the lyophilized exosome product can be applied to a pharmaceutical composition, a skin external preparation and a cosmetic composition. For example, the lyophilized exosome product can be used as a solution obtained by simply mixing it with a diluent.

The invention provides a protein chip stabilizing agent as well as a preparation method and application thereof. The protein chip stabilizing agent is prepared from saccharose, bovine serum albumin, Tween-20, trehalose and a biological preservative Proclin 300 in a PBS buffer system. The protein chip stabilizing agent prepared by the preparation method is capable of prolonging the protein immune activity storage life and reducing the cost, is safe and effective and contains no other chemical product; the saccharose and the trehalose are remarkably in synergistic effect, the stability of each of the saccharose and the trehalose can be respectively coordinated, and the stability of a whole protein chip can be more effectively increased; the protein immune activity of the protein chip in a kit during the storage life cannot be changed; a formula is simple, the cost is low, the application range is wide, no potential safety hazard exists, and good stabilizing effect and a wide application prospect are obtained.

There are provided trehalose transporter gene and a method of introducing trehalose into cells by using the gene. Candidates for the trehalose transporter genes were searched in P. vanderplanki EST, resulting in being obtained cDNA designated as Tret1. Tret1 encodes a 504 amino acid protein with 12 trans-membrane structures. Tret1 expression was induced by desiccation stress and predominant in the fat body. Functional expression of TRET1 in Xenopus oocytes showed that transport activity was specific for trehalose and independent of extracellular pH and electrochemical membrane potential. The direction of transport of TRET1 was reversible depending on the concentration gradient of trehalose. Apparent Km and Vmax of TRET1 for trehalose were extraordinarily high values. These results indicate that TRET1 is a facilitated, high-capacity trehalose-specific transporter. Tret1 is widespread in insects. Furthermore, TRET1 conferred trehalose permeability upon cells including those of vertebrates as well as insects.

The invention discloses an efficient active culture medium capable of increasing the content of a mushroom protein. The efficient active culture medium comprises the following components in parts by weight: soybean antigen proteins, triammonium citrate, humic acid, ferrous sulfate, polypropylene glycol, dandelion, folium cortex eucommiae, trehalose, a pH regulator, microelements, bamboo saw dust, loofah sponge, carboxymethyl cellulose sodium, soybean meal, bacillus subtilis, neutral protease, coco coir, pelelith and yeast. The mushroom culture medium prepared by taking enzymolysis high-protein soybean meal and the like as main materials is rich in nutrient and high in available protein content; the adsorption distribution of nutrient components and microelements of the culture medium as well as the osmotic pressure condition in the culture medium are regulated by using a loofah sponge loaded yeast compound and a culture medium conditioner with loofah sponge as a carrier through effective temperature-controlled fermentation, so that nutrient substances in the culture medium are accelerated to be absorbed and utilized by the mushroom; and the contents of the proteins and the microelements in the mushroom cultured by using the culture medium reach up to the standard, so that the demands of people are met.

The invention discloses an environment-friendly production method for enhancing trehalose yield of beer waste yeast under stress conditions, belonging to the field of biochemical engineering and aiming to solve the problems of long trehalose extraction time, low extraction rate and difficulty in purification due to too many impurities dissolved in the prior art. The method comprises the following steps: 1) microwave treatment; 2) heat treatment; 3) helicase treatment; 4) ultrasonic crushing treatment; and 5) trehalose extraction and purification. According to the method, the yeast is under stress conditions to generate abundant trehalose; meanwhile, the activity of the trehalase is inhibited, and the yeast is subjected to helicase treatment and ultrasonic crushing treatment to rupture the yeast cells, so that almost all the trehalose is dissolved out; and thus, the method has the advantages of short treatment time, easy trehalose purification and high yield. Besides, the method does not use any strong acid, strong alkali or toxic organic solvent, and implements cleanness and environmental protection; and the waste liquid and waste residue generated in the method can be used for producing yeast extract, so almost no waste liquid or waste residue is discharged.

The invention discloses a polymerase chain reaction enhancing method. The polymerase chain reaction enhancing method includes the steps of adding a PCR (polymerase chain reaction) enhancer into a PCR amplification system so as to obtain an enhanced PCR amplification system, wherein the PCR enhancer refers to a mixture of hexanehexol and trehalose; setting program parameters of the enhanced PCR amplification system, and conducting amplification test so as to obtain an amplified product; testing the amplified product on a fluorescence quantitative PCR amplification instrument. The polymerase chain reaction enhancing method has the advantages that since the mixture of the hexanehexol and the trehalose is used as the PCR enhancer, nucleic acid amplification reaction efficiency and / or yield can be remarkably improved and / or increased, and the nucleic acid sensitivity detected by nucleic acid amplification is improved.

The invention provides stem cell cryopreservation liquid and a preparation method thereof. The stem cell cryopreservation liquid is prepared from the following substances in percentage by volume: 35 to 55 percent of low-sugar type DMEM (Dulbecco's Modified Eagle Medium), 15 to 35 percent of Ultroser G serum replacement, 5 to 20 percent of glycerol, 5 to 10 percent of recombinant human serum albumin and 10 to 20 percent of dextran 40; the stem cell cryopreservation liquid further comprises 4 to 10mg / L of a bFGF (basic Fibroblast Growth Factor) and 250 to 700mmol / L of non-crystalline amorphous trehalose; the invention further provides the production method of the stem cell cryopreservation liquid. The stem cell cryopreservation liquid provided by the invention has the beneficial effects thatthe stem cell cryopreservation liquid is stable and reliable, a differentiation potential of cells can be effectively protected for long time and the cell bioactivity is ensured; exogenous microorganism infection and allergic risks are reduced; the production method is simple to operate and feasible, and has relatively good practical value.

A dehydrated composition is provided that includes freeze-dried platelets. The platelets are loaded with trehalose which preserves biological properties during freeze-drying and rehydration. The trehalose loading is conducted at a temperature of from greater than about 25° C. to less than about 40° C., most preferably at 37° C., with the loading solution having trehalose in an amount from about 10 mM to about 50 mM. These freeze-dried platelets are substantially shelf-stable and are rehydratable so as to have a normal response to an agonist, for example, thrombin, with virtually all of the platelets participating in clot formation within about three minutes at 37° C.

The invention relates to the technical field of total bilirubin detection, especially to a serum total bilirubin (oxidase method) detection reagent. A reagent R1 contains a buffer solution, lipase, ascorbic acid oxidase, alpha-cyclodextrin, sucrose, trehalose, PEG-6000, castor oil polyoxyethylene ether (EL-20) and a preservative. A reagent R2 contains a buffer solution, bilirubin oxidase, sucrose, trehalose, PEG-6000, castor oil polyoxyethylene ether (EL-20) and a preservative. The interference of lipid turbidity and ascorbic acid oxidase can be effectively avoided and the anti-interference ability of the reagent can be greatly enhanced by optimizing a reaction system and adding the lipase, the alpha-cyclodextrin and the ascorbic acid oxidase. The adoption of the novel PIPES(piperazine-1,4-diethylsulfonic acid) buffer solution and the addition of stabilizers such as the sucrose, the trehalose, the polyethylene glycol 6000 can effectively improve the stability of the reagent. Moreover, the addition of the optimal novel non-ionic surfactant castor oil polyoxyethylene ether (EL-20) can prevent the reaction system from turbidity and further improve the stability and the anti-interference ability of the reagent.

The object of the present invention is to provide a composition of metal ion compound whose inherent and unsatisfactory properties for its industrial application such as deliquescence, reducing power, oxidizing power, low solubility in water, etc., are improved; and their preparation and uses. The present invention solves the above object by providing an associate of trehalose or maltitol and a metal ion compound or bittern component, and their preparation and uses.

The invention relates to a crystallization technical method for industrially producing trehalose. According to the crystallization technical method for industrially producing the trehalose, in industrial production, by utilizing the characteristics of trehalose mixed sugar liquid, crystalline trehalose is produced quickly and largely by adopting the following four steps of (1), performing high-temperature low-pressure sugar boiling: quickly concentrating the trehalose mixed sugar liquid until a highest critical concentration at which a crystal is separated out is reached; (2), quickly cooling and crystallizing: putting the trehalose mixed sugar liquid into a crystallization tank, and carrying out the quick cooling and crystallization in a natural condition without adding any eluting agent and crystal seed; (3), replenishing an appropriate amount of water for dissolving heterogeneous sugar: when the trehalose mixed sugar liquid is cooled to be at a room temperature and the crystal is completely separated out, adding cold pure water in a certain proportion into the trehalose mixed sugar liquid, so as to dissolve most of the heterogeneous sugar on the surface of the crystal; (4), centrifuging an obtained mixture at a low speed, and naturally air-drying an obtained product, so as to obtain high-purity crystalline trehalose, and completely recycling and reusing centrifuged mother liquid. The method provided by the invention has the advantages of being short in period, high in efficiency, low in energy consumption, free from pollution and zero in release; the product made by the crystallization technical method is safe and a user can be unworried.

The invention relates to a trehalose modified polyvinyl alcohol anti-fog and anti-frost coating and a preparation method thereof. The coating is prepared from polyvinyl alcohol-grafted-trehalose, low polyethylene glycol dimethacrylate, benzoin dimethyl ether and deionized water; the materials are uniformly mixed and coated on a transparent glass sheet, and the mixture is irradiated and cured by ultraviolet light to form the hydrophilic graft polymer semi-interpenetrating network coating. According to the prepared anti-fog and anti-frost coating, the light transmittance can be kept at 89-90% in the anti-fog process, the anti-frost time can be prolonged by 9-31 min, and the light transmittance value of the glass sheet coated with the coating on the two sides at the wave band of 400-800 nm is calculated by using a visible spectrophotometer. Good anti-fog and anti-frost performance is realized. The preparation method is simple and convenient and mild in reaction, and has great application potential in the field of fog and frost prevention.

The invention discloses a medicine preparation containing an anti-CD20 antibody as well as a preparation method and an application of the medicine preparation. The medicine preparation comprises 1) 50-200 mg / ml anti-CD20 antibody; 2) 10-50 nM buffering agent which is a histidine buffering agent; 3) 105-420 mM alpha alpha-trehalose dihydrate; 4) 5-20 mM methionine and 5) 0.01%-0.12% of a nonionic surfactant; the medicine preparation containing the anti-CD20 antibody has the pH being 5.5 plus or minus 2.0; and preferably, the medicine preparation also contains hyaluronidase. The medicine preparation containing the anti-CD20 antibody has excellent stability.

The invention discloses a preparation method of high-content trehalose. The preparation method of the high-content trehalose sequentially comprises the following steps: (1), preparing starch milk; (2), liquefying; (3), performing double enzymolysis; (4), filtering to remove protein; (5), decolorizing; (6), desalting; (7), performing nanofiltration and chromatographic separation purification; (8),performing concentration crystallization; and (9), separating and drying to obtain a finished product. The preparation method has the advantages as follows: the prepared product has the trehalose content being higher than 99.7%, and is high in functionality; compared with an ordinary trehalose product, the using amount of the high-content trehalose for achieving the same effect can be reduced by 2-5%; for baking, the high-content trehalose can increase the bulkiness of a baked product, and for a cosmetic, the high-content trehalose can improve the moisture retention of the cosmetic; occurrenceof a maillard reaction can be effectively reduced; the product provided by the invention is high in yield, so that the output of an enterprise can be increased and industrial production is facilitated; the product provided by the invention is relatively uniform in granularity and has a smaller granule size than the ordinary trehalose, is high in dissolution speed and convenient to use.