79results about How to "Reduce separation and purification steps" patented technology

The invention discloses a method for producing L-2-aminobutyric acid by double immobilized multi-enzyme systems. The method provided by the invention comprises the following steps that 1, threonine dehydrogenase and leucine dehydrogenase are fixed on reversely-dissoluble pH-sensitive polymer carriers so that a co-immobilized multi-enzyme system is obtained; and 2, alcohol oxidase, formaldehyde dehydrogenase and formate dehydrogenlyase are fixed on reversely-dissoluble pH-sensitive polymer carriers so that a co-immobilized coenzyme regeneration system is obtained. The method for producing L-2-aminobutyric acid by the double immobilized multi-enzyme systems utilizes dissolution reversibility of co-immobilized enzymes, realizes effective separation of the co-immobilized enzymes and products, improves accessibility between the co-immobilized enzymes and reactants, improves recovery and utilization rates of threonine dehydrogenase, leucine dehydrogenase, alcohol oxidase, formaldehyde dehydrogenase and formate dehydrogenlyase, improves coenzyme regeneration efficiency, reduces follow-up separation purification processes, simplifies a process flow and reduces a production cost.

The invention relates to a novel method for preparation and purifying an electrolyte lithium salt LiDFOB for a lithium ion battery, which belongs to the technical fields of novel energy materials and preparation. The method comprises the following specific steps of: (1) dissolving anhydrous dry NaBF4 and LiCl into an organic solvent; (2) adding a catalyst, heating, stirring and reflowing at the temperature of 30-80 DEG C, and fully reacting for 4-12 hours; (3) filtering, separating a solid from a liquid to obtain an organic solution of LiBF4, adding H2C2O4 and a catalyst in an inertial atmosphere, performing a magnetic stirring reaction at the temperature of 30-120 DEG C for 4-12 hours till no gas is generated, and ending reaction; and (4), filtering, evaporating a filtrate with a rotary evaporimeter till white solid particles are just formed, adding a non-polar solvent into the white solid particles, recrystallizing at a low temperature, filtering, and drying in vacuum at the temperature of 60 DEG C to obtain a high-purity LiDFOB product. The LiDFOB synthesizing method is simple, used raw materials are safe and nontoxic, an intermediate, i.e., LiBF4 is not required to be separated from a solvent, and the process flow is simplified. The LiDFOB prepared by using the process has superior performance, has a good application prospect in the field of power batteries, and is convenient to industrialize.

The invention discloses a gene for coding a glutamine dipeptide biosynthetic enzyme and application thereof. The nucleotide sequence of the gene is shown as SEQ ID NO.1. The invention further discloses an amino acid sequence coded by the gene, and provides a recombinant vector containing the gene, recombinant escherichia coli, and a method for performing biotransformation to synthesize the glutamine dipeptide by using the gene. The gene and the application of a recombinant bacterial strain of the gene have the advantages of high mol conversion rate, high reaction speed, easy separation, low cost and the like. In the synthesis method, the maximum mol conversion rate of the glutamine dipeptide can reach 83.3 percent; meanwhile, the thalli can be applied to the catalytic synthesis of the glutamine dipeptide again after the circulation recovery; in addition, the catalytic activity is stable. Therefore high market competitive power and application values are realized; a foundation is laid for the industrial production of the glutamine dipeptide.

The invention discloses a method for extracting active alkaloid from lycoris plants. The method utilizes the combination of an enzymic method and modern mechanical wall-breaking technique to extract lycoris alkaloid. Lycoris alkaloid is extracted efficiently in excellent yields under mild conditions, other impurities dissolution is lowered and the consistency of extract is decreased for continuing treatment of isolation and purification. Segmental extraction technique is adopted according to the alkalinity of lycoris alkaloid to get a plurality of raw products of lycoris alkaloid. Then highlypurified monomer of lycoris alkaloids (galanthamin, galanthidine, dihydrogalanthamine, sekisamine) are available after column chromatography and repeated recrystallization. The invention has the advantages of simple line, no pollution, short cycle, high yield of products, high safety coefficient, low cost, diversity of outputs, thus raising the comprehensive utilization of lycoris and reducing the manufacturing cost of deep processing and increasing the feasibility of industrial production.

The invention discloses choline eutectic solvents, a preparation method and an application in extraction of flavone C-glycoside of flos trollii. The choline eutectic solvents have the expression: [N(CH)33CH2CH2OH]+[X.zY]- and is prepared from choline bromide or choline chloride and ZnCl2, SnCl2 or AlCl3 through mixing in a certain mole ratio, wherein when X is Br, Y is ZnCl2 or SnCl2; when X is Cl, Y is AlCl3. By means of the provided choline eutectic solvents, three important flavone C-glycosides, namely, orientin, orientin-2''-O-beta-L-galactoside and vitexin in the flos trollii can be efficiently and selectively extracted; compared with conventional extraction with methanol solvents, the extraction rate of orientin, orientin-2''-O-beta-L-galactoside and vitexin can be improved, few impurities exist in an extract, silica gel columns are not required to be repeatedly used for separation when the extract serving as the raw material is used for preparing monomers of orientin, orientin-2''-O-beta-L-galactoside and vitexin, the separation and purification steps can be greatly reduced, and the separation and purification cost can be reduced.