Choline deep eutectic solvent, preparation method and application in extracting flavonoid carbon glycosides of nasturtium
A technology of deep eutectic solvent and flavonoid carbon glycosides, which is applied in the field of chemical medicine, can solve problems such as environmental damage, achieve the effects of less impurities, reduce separation and purification steps, and reduce separation and purification costs
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Embodiment 1
[0022] Embodiment 1: the preparation of cholines deep eutectic solvent
[0023] Different types of choline deep eutectic solvents and preparation methods are as follows:
[0024]
Embodiment 2
[0025] Example 2: Application of choline-based deep eutectic solvent in extracting flavonoid carbon glycosides from Trollica chinensis
[0026] 1. Experimental method
[0027] 1. Extraction of flavonoid glycosides from Trollica chinensis
[0028] After the nasturtium is dried, it is crushed and passed through a 50-mesh sieve. Accurately weigh 2 g of nasturtium powder, add the serial number 1-6 eutectic solution-water mixed solution (3:1, v / v) prepared in 40ml embodiment 1, ultrasonic treatment (power 250W, frequency 40kHz) 20min, under room temperature After cooling, centrifuge at 13000rpm for 10min, take the supernatant, and dilute the supernatant 10 times with water, then place it in the injection vial as the test solution, and wait for HPLC detection. The remaining supernatant was diluted with 5 times the volume of ethanol to precipitate the deep eutectic solvent, filtered through a 0.45 μm organic filter, and the filtrate was concentrated to obtain the nasturtium extract...
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