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Preparation method of high-content trehalose

A trehalose, high-content technology, applied in the preparation of sugar derivatives, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of inability to completely remove, limit the scope of application of trehalose, and improve the degree of water locking and moisturizing. , the effect of reducing Maillard reaction and high yield

Active Publication Date: 2018-06-08
TONGLIAO MEIHUA BIOLOGICAL SCI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, for the improvement of trehalose content, the methods of ion exchange desalination and chromatographic separation and purification are generally adopted, and the prepared trehalose content is about 98%; or refined by alcohol separation, but alcohol separation needs to use some chemical reagents, such as Alcohol substances, which have a certain amount of retention in trehalose products, cannot be completely removed. Although the content of trehalose in the product has been increased, the scope of application of trehalose products has been limited to a certain extent. Trehalose products cannot be used in cosmetics

Method used

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  • Preparation method of high-content trehalose
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  • Preparation method of high-content trehalose

Examples

Experimental program
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Effect test

Embodiment 1

[0020] A method for preparing high-content trehalose, which specifically includes: (1) preparation of starch milk, (2) liquefaction, (3) double enzymatic hydrolysis, (4) filtering to remove protein, (5) decolorization, (6) desalination, (7) Purification, (8) concentration and crystallization, (9) separation and drying; wherein:

[0021] (1) Preparation of starch milk: Soak corn in water, the ratio of corn to water is 5:2, the temperature of soaking water is 40°C, and soak until the seed coat of corn can be peeled off to obtain soaked corn; filter out the water and dry the soaked corn Crushing is carried out to separate the seed coat until the whole grain amount is 8 grains / 100g to obtain broken corn; the broken corn is ground to remove the germ in the corn until the number of corn germ in the grinding liquid is 3 pieces / 100ml to obtain the grinding liquid ; Separating the discs from the grinding liquid until the protein content in the grinding liquid is 10%, to obtain starch m...

Embodiment 2

[0032] A method for preparing high-content trehalose, which specifically includes: (1) preparation of starch milk, (2) liquefaction, (3) double enzymatic hydrolysis, (4) filtering to remove protein, (5) decolorization, (6) desalination, (7) Purification, (8) concentration and crystallization, (9) separation and drying; wherein:

[0033](1) Preparation of starch milk: Soak corn in water, the ratio of corn to water is 6.6:4.5, the temperature of soaking water is 50°C, and soak until the seed coat of corn can be peeled off to obtain soaked corn; Crushing is carried out to separate the seed coat until the whole grain amount is 5 grains / 100g to obtain broken corn; the broken corn is ground to remove the germ in the corn until the number of corn germ in the grinding liquid is 2 / 100ml to obtain the ground liquid; the grinding liquid is subjected to disc separation until the protein content in the grinding liquid is 6%, and starch milk is obtained;

[0034] (2) Liquefaction: (1) Afte...

Embodiment 3

[0044] A method for preparing high-content trehalose, which specifically includes: (1) preparation of starch milk, (2) liquefaction, (3) double enzymatic hydrolysis, (4) filtering to remove protein, (5) decolorization, (6) desalination, (7) Purification, (8) concentration and crystallization, (9) separation and drying; wherein:

[0045] (1) Preparation of starch milk: Soak corn in water, the ratio of corn to water is 8:5, the soaking water temperature is 60°C, and soak until the seed coat of corn can be peeled off to obtain soaked corn; filter out the water and dry the soaked corn Carry out crushing until the whole grain amount is 2 grains / 100g to obtain broken corn; grind the broken corn (remove the germ in the corn), until the number of corn germs in the grinding liquid is 1 piece / 100ml, obtain the grinding liquid; grinding liquid Carry out disk separation until the protein content in the grinding liquid is 3%, to obtain starch milk;

[0046] (2) Liquefaction: (1) After the...

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Abstract

The invention discloses a preparation method of high-content trehalose. The preparation method of the high-content trehalose sequentially comprises the following steps: (1), preparing starch milk; (2), liquefying; (3), performing double enzymolysis; (4), filtering to remove protein; (5), decolorizing; (6), desalting; (7), performing nanofiltration and chromatographic separation purification; (8),performing concentration crystallization; and (9), separating and drying to obtain a finished product. The preparation method has the advantages as follows: the prepared product has the trehalose content being higher than 99.7%, and is high in functionality; compared with an ordinary trehalose product, the using amount of the high-content trehalose for achieving the same effect can be reduced by 2-5%; for baking, the high-content trehalose can increase the bulkiness of a baked product, and for a cosmetic, the high-content trehalose can improve the moisture retention of the cosmetic; occurrenceof a maillard reaction can be effectively reduced; the product provided by the invention is high in yield, so that the output of an enterprise can be increased and industrial production is facilitated; the product provided by the invention is relatively uniform in granularity and has a smaller granule size than the ordinary trehalose, is high in dissolution speed and convenient to use.

Description

Technical field: [0001] The invention relates to a method for preparing trehalose, in particular to a method for preparing high-content trehalose. Background technique: [0002] Trehalose, also known as sucrose, mushroom sugar, etc., is a safe and reliable natural sugar. Trehalose has a magical protective effect on organisms. Trehalose can form a unique protective film on the cell surface under harsh environmental conditions such as high temperature, high cold, high osmotic pressure, and dehydration, effectively protecting protein molecules from denaturation and inactivation. , so as to maintain the life process and biological characteristics of living organisms. However, other sugars such as sucrose and glucose in nature do not have this function. This unique functional property makes trehalose not only an excellent active protectant for protein drugs, enzymes, vaccines and other biological products, but also an important ingredient for maintaining cell activity and moist...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/24C12P19/14C12P19/12C07H3/04C07H1/06
CPCC12P19/12C12P19/14C12P19/24C07H1/06C07H3/04C12Y302/01141C12Y504/99015
Inventor 伊婷婷富春丽齐志伟李军范世远
Owner TONGLIAO MEIHUA BIOLOGICAL SCI TECH CO LTD
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