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Cryoprotectant based on liquid metal nanoparticles as well as method and application of cryoprotectant

A technology of cryoprotectant and liquid metal, which is applied in the field of biomedicine to achieve the effect of uniform particle size distribution, reduced damage, and large application prospects

Active Publication Date: 2020-02-28
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During rewarming, the critical heating rate must exceed the critical cooling rate by at least an order of magnitude to prevent ice crystal formation in the cryoprotectant medium and system, and achieving faster warming rates at low concentrations of cryoprotectants remains challenging

Method used

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  • Cryoprotectant based on liquid metal nanoparticles as well as method and application of cryoprotectant
  • Cryoprotectant based on liquid metal nanoparticles as well as method and application of cryoprotectant
  • Cryoprotectant based on liquid metal nanoparticles as well as method and application of cryoprotectant

Examples

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Embodiment 1

[0024] This embodiment provides a freezing balance solution, cryoprotectant and thawing solution for human bone marrow mesenchymal stem cells. The components of the freezing balance solution are: conventional culture solution and basic protection agent. The conventional culture solution is composed of 40% human bone marrow mesenchyme It is composed of stem cell complete medium and 60% fetal bovine serum. The basic protective agent is ethylene glycol and propylene glycol, both of which have a concentration of 1M. The cryoprotectant components are: conventional culture medium and basic protective agent. The conventional culture medium is composed of 40% human bone marrow mesenchymal stem cell complete medium and 60% fetal bovine serum. The basic protective agents are ethylene glycol, propylene glycol and trehalose. The concentration of ethylene glycol and propylene glycol is 2M, and the concentration of trehalose For 0.5M. The thawing fluid component is 0.5M trehalose in PBS.

Embodiment 2

[0026] This embodiment provides a freezing balance solution, cryoprotectant and thawing solution for human bone marrow mesenchymal stem cells. The components of the freezing balance solution are: conventional culture solution and basic protection agent. The conventional culture solution is composed of 40% human bone marrow mesenchyme It is composed of stem cell complete medium and 60% fetal bovine serum. The basic protective agent is ethylene glycol and propylene glycol, both of which have a concentration of 1M. The cryoprotectant components are: liquid metal nanoparticles, conventional culture medium and basic protective agent. The liquid metal nanoparticles are GaIn 24.5 The alloy (average particle size is 100-200nm) has a concentration of 0.1 mg / mL. The conventional culture medium consists of 40% human bone marrow mesenchymal stem cell complete medium and 60% fetal bovine serum. The basic protective agent is ethylene glycol, propylene glycol and The concentration of trehalos...

Embodiment 3

[0028] This embodiment provides a cryoprotectant for human bone marrow mesenchymal stem cells. The preparation method is the same as that in Example 2, except that the concentration of the nano liquid metal is 0.2 mg / mL.

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Abstract

The invention relates to a cryoprotectant based on liquid metal nanoparticles as well as a method and an application of the cryoprotectant. The cryoprotectant comprises the liquid metal nanoparticlesand a basic protectant, wherein the melting point of the raw material of the liquid metal nanoparticles is below 60 DEG C, and the basic protective agent is a mixture of ethylene glycol, propylene glycol and trehalose. The cryoprotectant can realize vitrification in the cooling process, and can be used as a space heat source under the irradiation of near-infrared laser in the rewarming process, thereby greatly enhancing the heating rate, inhibiting anti-vitrification, reducing the formation of ice crystals, and enhancing the survival rate and structural integrity of cryopreserved cells and vascular tissues.

Description

Technical field [0001] The present invention relates to the field of biomedicine, in particular to a cryoprotectant based on liquid metal nanoparticles, and a method and application thereof. Background technique [0002] Cryopreservation is the only method for long-term preservation of valuable cells to maintain cell functional properties (such as immunomodulatory ability, differentiation ability) and reach the number required for clinical applications. Recently, various methods for cryopreservation have been developed, including slow freezing and vitrification. The slow freezing method is time-consuming and usually requires expensive program cooling equipment to achieve the required cooling rate. Compared with slow freezing, because vitrification is a freezing mode that quickly reduces the cell temperature, it avoids the mechanical damage to the cells during the slow freezing process, while shortening the freezing processing time, simplifying the steps, and eliminating the need...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02B22F1/00B22F9/06
CPCA01N1/0221B22F9/06B22F1/054
Inventor 后仪饶伟刘静
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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