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Production method for powder containing crystalline alpha, alpha-trehalose dihydrate

A technology of dihydrate and crystalline powder, which is applied in the preparation of sugar derivatives, biochemical equipment and methods, sugar derivatives, etc., can solve the problems such as inability to obtain, and achieve easy cultivation, high crystallinity, and excellent starch yield. Effect

Active Publication Date: 2014-07-23
HAYASHIBARA CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is an inconvenience that in the case of the whole sugar method, the crystallized trehalose and the common impurities peculiar to such a production method of glucose contained in the massecuite are all pulverized together, and therefore, the obtained trehalose-containing The trehalose content in the powder of dihydrate crystals is not higher than the trehalose content of massecuite, and the powder containing high-purity trehalose dihydrate crystals cannot be obtained

Method used

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  • Production method for powder containing crystalline alpha, alpha-trehalose dihydrate
  • Production method for powder containing crystalline alpha, alpha-trehalose dihydrate
  • Production method for powder containing crystalline alpha, alpha-trehalose dihydrate

Examples

Experimental program
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Effect test

Embodiment 1

[0275]

[0276] Corn starch was suspended in water so as to be 30%, and calcium carbonate was added to this suspension to have a final concentration of 0.1%, and the pH was adjusted to 6.0. To this suspension, add 0.2% based on the mass of starch of heat-resistant α-amylase (trade name "Kumamamil 60L", sold by Nobozaimzu Japan Co., Ltd.), react at 98 to 100°C for 15 minutes, and Starch gelatinization - liquefaction. After the obtained liquefied starch solution was treated in an autoclave at 125°C for 15 minutes, it was cooled to 51°C. To this solution, 2 units and 10 units of α- Partially refined enzyme solution of glycosyl trehalose synthase and trehalose separation enzyme, in addition, 300 units of isoamylase (manufactured by Hayashibara Co., Ltd.) per 1 gram of starch, and 2 units of isoamylase derived from experiments 1-2 The prepared CGTase of Paenibacillus illinois NBRC15959 strain was further reacted for about 70 hours. Next, this reaction solution was heated at 97°...

Embodiment 2

[0279]

[0280] Using 3 units and 15 units of α-glycosyl trehalose synthase and trehalose separation enzyme per 1 gram of starch respectively, the reaction time reaches 40 hours. Except for the CGTase of Paenibacillus NBRC15379 strain, trehalose production reaction and glucoamylase treatment were performed in the same manner as in Example 1, thereby obtaining the purity of trehalose, that is, the purity of trehalose in terms of anhydrous matter. A reaction solution with a trehalose content of 87.6%. Heating the obtained reaction solution to inactivate the enzyme, decolorize and filter the filtrate through activated carbon according to conventional methods, desalt the filtrate with cation exchange resin (H+ type) and anion exchange resin (OH- type), and concentrate under reduced pressure to form a solid concentration of about 85 % concentrate. Put it in an auxiliary crystallization tank, add 1% reagent-grade powder containing trehalose dihydrate crystals (trade name "trehalo...

Embodiment 3

[0284]

[0285]As the CGTase, except that the CGTase derived from the Paenibacillus NBRC13638 strain prepared by the method of Experiment 1-2 was used, in the same manner as in Example 1, the trehalose production reaction was performed, and then the glucoamylase The trehalose content of the reaction liquid after the treatment was 87.2% in terms of anhydrous. The reaction liquid thus obtained is heated to inactivate the enzyme, and decolorized and filtered through activated carbon according to a conventional method, and the filtrate is desalted with a cation exchange resin (H+ type) and an anion exchange resin (OH- type), and concentrated under reduced pressure to form a solid concentration About 85% concentrate. Put it in an auxiliary crystallization tank, add 1% reagent-grade powder containing trehalose dihydrate crystals (trade name "trehalose 999", code number: TH224, trehalose purity 99.9% or more, sold by Hayashibara Co., Ltd.) As a seed crystal, bring it to 60°C while...

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Abstract

Provided is a production method whereby it is possible to produce a powder containing crystalline trehalose dihydrate with starch as a starting material with high powder-to-starch yields. More specifically, provided is a production method for a powder containing crystalline alpha, alpha-trehalose dihydrate and having at least 98.0mass% of alpha, alpha-trehalose on an anhydrous basis, said method including: a step in which liquefied starch is subjected to the actions of a starch debranching enzyme, cyclomaltodextrin glucanotransferase, an alpha-glycosyl trehalose producing enzyme derived from a micro-organism belonging to the genus arthrobacter, and a trehalose releasing enzyme derived from a micro-organism belonging to the genus arthrobacter, and then to the action of glucoamylase, to obtain an alpha, alpha-trehalose-containing saccharide solution; a step in which alpha, alpha-trehalose dihydrate is crystallized from the saccharide solution; and a step in which the crystallized alpha, alpha-trehalose dihydrate is extracted by centrifugation, aged, and dried. The production method is characterized in that a natural or recombinant enzyme derived from a micro-organism belonging to the genus paenibacillus or a variant of such an enzyme is used as the cyclomaltodextrin glucanotransferase such that the content of alpha, alpha-trehalose in the saccharide solution is over 86.0mass% on an anhydrous basis, without having to undergo a column chromatography fractionation step.

Description

【Technical field】 [0001] The present invention relates to a method for preparing a powder containing α,α-trehalose dihydrate crystals, in particular, to a method for preparing a powder containing α,α-trehalose dihydrate crystals and the product obtained by the preparation method Powder containing α,α-trehalose dihydrate crystals, said method is suitable for industrially producing high-purity α,α-trehalose dihydrate crystals containing powders from starch through successive steps in excellent yield powder. 【Background technique】 [0002] As a method for producing a powder of dihydrate crystals containing α,α-trehalose (hereinafter, this specification is simply referred to as “trehalose”), various methods have hitherto been known. For example, Patent Document 1 discloses a method for preparing a powder containing trehalose dihydrate crystals by allowing β-amylase or β-amylase and isoamylase to act on liquefied starch, followed by making maltose / trehalose Invertase acts on li...

Claims

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Application Information

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IPC IPC(8): C12P19/12C07H3/04C12P19/16C12P19/18C12P19/20C12N9/10
CPCC07H3/04C12P19/18C07H1/00C12P19/14C12N9/1074C12Y204/01019C12P19/12C12P19/20C13K13/007
Inventor 涩谷孝伊泽精祐
Owner HAYASHIBARA CO LTD
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