Detecting reagent of urea nitrogen and detecting test paper of urea nitrogen
A detection reagent and detection test paper technology, which is applied in the field of urea nitrogen detection, can solve the problems of high material requirements, complex process, and the detection accuracy needs to be improved, and achieves strong anti-interference ability, eliminates interference, and ensures fast and relatively accurate detection. Effect
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Embodiment 1
[0055] A kind of detection test paper 1 of urea nitrogen, its preparation method comprises the following steps:
[0056] Step 1: Paste double-sided adhesive tape 200 on the reactive base layer 100 made of PET material with a certain hardness, and punch through holes 110 for later use.
[0057] Step 2. Tear off the oil paper of the double-sided adhesive 200 on the reaction base layer 100, and paste the film material 320 on the double-sided adhesive tape 200 of the PET board. The film material 320 needs to completely cover the through hole 110 on the PET substrate.
[0058] Step 3: First prepare the detection reagent 310: including 50KU / L urease, 1.0g / L trehalose, 20g / L chromogenic substance, 0.15g / L BSA, 0.1g / L sodium chloride. Add the prepared detection reagent 310 dropwise to the corresponding through hole 110 of the membrane material 320 with a dispenser. After 20 minutes, the reaction layer 300 was obtained.
[0059] Step 4: Paste a blood filter membrane on the reaction l...
Embodiment 2
[0062] In the preparation method of urea nitrogen detection test paper 1 in this embodiment, the detection reagent 310 includes 55KU / L urease, 1.2g / L trehalose, 22g / L chromogenic substance, 0.20g / L BSA, 0.12g / L Sodium chloride. Other steps are all the same as in Example 1.
Embodiment 3
[0064] In the preparation method of urea nitrogen detection test paper 1 in this embodiment, the detection reagent 310 includes 57.5KU / L urease, 1.3g / L trehalose, 25g / L chromogenic substance, 0.15g / L BSA, 0.13g / L of sodium chloride. Other steps are all the same as in Example 1.
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