162results about How to "Meet research needs" patented technology

The invention relates to an intelligent cutter of an integrated small three-way cutting force measurement system, belonging to the fields of ultraprecise cutting machining and cutting force measurement and real-time monitoring. The intelligent cutter can realize the real-time sensing measurement of small three-way cutting force in the ultraprecise machining process. The intelligent cutter mainly comprises a cutter bar, a cutting blade, a cutting force measurement system, a sealing cover and a signal interface end, wherein the upper surface of the cutting blade coincides with the central surface of the main body of the cutter bar; the cutter tip is positioned in the center of the main body of the cutter bar; the front end of the main body of the cutter bar is provided with a sensing measurement section; the small three-way cutting force measurement system is fixedly connected in a force measurement cavity of the sensing measurement section; the signal interface end is arranged at the back end of the cutter bar and is used for realizing signal output; and in the cutting machining process, a sensing unit senses the strain and displacement generated under the effect of the three-way cutting force to acquire a corresponding signal, and the small three-way cutting force is accurately computed through calibration and decoupling. The invention is used for related scientific researches in the field of ultraprecise machining and real-time monitoring and self-adaptive machining in the cutting process.

The invention discloses a system for identifying and evaluating seedling resistance of malus. A method thereof comprises the following steps: cultivating seedlings; transplanting the seedlings and performing aquiculture; performing stress treatment; surveying and classifying disaster condition; establishing an evaluating system, and the like. By using the method, the traditional land identification result is replaced by a seedling identification result, the identification period is greatly shortened, the land and the test cost are saved, the influence of external environment on the identification result is efficiently avoided, a technical system suitable for identifying and evaluating the resistance of the malus is supplied, and an important theoretic reference is supplied for standardizing the identifying and evaluating system for germ plasm resources of apples in China, ensuring the accuracy of the identification and evaluation result and the effectiveness of resource utilization. The method has stronger application potential in the aspects of screening the germ plasm resources of apples with strong resistance, cultivating resistive apples and researching the resistance mechanism of the malus.

The invention discloses an extraction and purification method of total plant endophyte genome DNA for colony analysis. The method comprises the following steps of: shearing and grinding the surface bacteria removed fresh plant tissues into paste, soaking the plant tissues into sterilized phosphate buffer solution, performing thermostatic shaking for 1 hour in a table concentrator to separate out microbial cells from the plant tissues, standing, transferring the suspension to a centrifuge tube, performing centrifugal collection on microbial bacteria, then adding lysing solution, shaking and uniformly mixing the solution to release DNA from the cells, repeatedly extracting protein by chloroform-isoamylol, performing centrifugal sedimentation with isopropanol and washing with 70 percent ice ethanol, performing purification by using a DNA specific centrifugal adsorption column to obtain total crude extracted genome DNA, and finally, performing polymerase chain reaction (PCR) amplificationby using special primers to obtain high-quality plant endophyte specific DNA fragments for subsequent analysis. The invention has the advantages that: the method is simple and effective, high in quality, low in pollution and the like, and provides high-quality guarantee for comprehensively and completely researching a plant endophyte colony structure.

The invention discloses a method for judging a fluid type of a reservoir by utilizing logging data of while-drilling drilling fluid, which comprises the following steps of: A, data collection, i.e., collecting performance parameters of the while-drilling drilling fluid of a target stratum, wherein the performance parameters comprise electrical conductivity, a temperature, drilling time and a hydrocarbon component of the drilling fluid; B, data processing; C, setting of a crossplot graph data track, i.e., for a plumb shaft, respectively combining an Sf data track and a Tf data track to form an S-T data track, combining the Sf data track and a DHf data track to form an S-DH data track and respectively making crossplots; D, judgment of the fluid type, i.e., using the S-T dat track for judging a dry layer (a micro gas-bearing bed) and an aqueous layer, using the S-DH data track for the plumb shaft to judge the gas-bearing bed or a gas reservoir and using a DO-DH data track for judging an oil-bearing formation or the gas-bearing bed. When the method is adopted, an explanation result of the method is objective and accurate, the coincidence rate of the explanation result and the oil testing conclusion is high, and the requirements on production and research can be met.

The invention discloses a double-bending-element ultrasonic sensing test device for evaluating rock damage. The double-bending-element ultrasonic sensing test device comprises a pressure chamber, a rock sample and an ultrasonic probe, wherein the pressure chamber comprises a housing, a pressure head and a base; the rock sample is positioned between the base and the pressure head; grooves are formed in the sides of the pressure head and the base, in contact with the rock sample; seat cores are arranged in the grooves; the ultrasonic probe comprises an ultrasonic emission probe and an ultrasonic receiving probe; the ultrasonic emission probe and the ultrasonic receiving probe are placed in the pressure head and the seat cores of the base respectively; each ultrasonic probe adopts a double-bending-element structure formed by bonding two bending elements in parallel; and a signal generator, a signal amplifier, an oscilloscope, a first circuit converter and a second circuit converter are arranged outside the pressure chamber. The connection mode of the two bending elements is changed through the circuit converters, the vibration directions of the bending elements are controlled for achieving sequential excitation of longitudinal waves and horizontal waves, the test device is suitable for a rock mechanic ultrasonic test, and the rock damage characteristic is evaluated through wave speed change.

The invention provides a pool testing method of low-frequency acoustic directivity of a large submerged buoy vector hydrophone. The pool testing method comprises the steps of (1) placing a submerged buoy system to the center of a pool, placing a low-frequency / very-low-frequency acoustic source to a position which enables the vector hydrophone in the submerged buoy system to be positioned in an acoustic source near field and the center of the vector hydrophone and the acoustic source to be positioned at the same placing depth, wherein the sound filed at the position of the vector hydrophone expands in a spherical wave mode; (2) determining the range of a rotary angle of the submerged buoy system according to the dimension of the submerged buoy system and equivalent conditions of testing results of the spherical wave and testing results of a plane wave; and (3) enabling the whole submerged buoy system to rotate at a low speed, recording signals when emission frequency of a sound pressure channel of the vector hydrophone is f0 in real time, and performing post-processing to obtain low-frequency / very-low-frequency acoustic directivity of the submerged buoy vector hydrophone. By means of the pool testing method, the testing of the acoustic directivity of the complicated vector hydrophone system can be achieved in a high precision mode in the pool which can be controlled easily, and accordingly, the requirements of further research are met.

This invention discloses an electric pump micro-cavity laser outputting laser from a port of an integrated straight waveguide based on a whisper cloister mode theory and composed of a die and heat sink, in which, the die is a chip processed in complecated processes of photoetch, corrosion, oxidation and evaporation of electronic beams on the epitaxial chip of a semiconductor quantum well laser, and the center of which is a micro-disk or a micro-loop, a straight waveguide of several mum wide, several decades-several hundreds long and same thick to the disk or the loop is integrated along the tangent of the edge of the disk or loop, in which, an ohmic contact metal film is prepared on the surface of the disk or the loop and a sloder is applied to weld the die and the heat sink.

The invention discloses a graphite surface titanium metallization method and a product manufactured with the same to solve the problem that when graphite is connected with Cu or Cu alloy, it is difficult to wet graphite with liquid metal, the difference between graphite and Cu or Cu alloy is large in terms of expansion factor and elasticity modulus, and connection is hard. According to the method, a titanium film is deposited on the surface of a graphite substrate with the magnetron sputtering method, and then hot isostatic pressing is conducted on the deposited titanium film, so that a titanium thin film is formed on the surface of the graphite substrate. The problem that coating-substrate combination strength of a surface titanium metallization layer is low during diffusion bonding of the non-metal material graphite can be effectively solved, and the metal titanium thin film high in coating-substrate combination strength can be prepared on the graphite substrate. According to the titanium thin film, the concentration of titanium becomes larger gradually from the graphite substrate to the outside in a gradient mode, and the strength of combination between a titanium coating and the substrate is high. The method can meet the requirement for research of fusion reactor plasma facing materials and has great significance for development of fusion reactor elements.

The invention discloses an acoustic and electric parameter measuring device used for loading procedure of gas-containing coal bodies. The measuring device includes a loading system, a gas circuit system, an acoustic parameter measuring system and an electric parameter measuring system, wherein the loading system includes a loading device capable of relatively and hermetically performing radial loading and axial loading, and a loading pump providing load for the loading procedure; the gas circuit system includes a methane gas bottle, as well as a gas inlet pipeline and a gas outlet pipeline that are communicated with the loading device respectively, wherein the methane gas bottle is connected onto the gas inlet pipeline; the acoustic parameter measuring system includes an acoustic probe, a sound generator and an oscilloscope; and further, the electric parameter measuring system includes an electrode plate, an insulation board and an LCR (inductance, capacitance and resistance) tester. Through adopting the structure, the measuring device can test different coal body acoustic and electric parameters as axial pressure, confining pressure and pore pressure, and provides a great deal of accurate test data and theoretical base.

The invention relates to a method for synthesizing bisphenol A-type medicine general artificial antigen, belonging to the biochemical technical field. The method takes diphenolic acid BHPVA as a hapten, couples the diphenolic acid BHPVA and a carrier protein, namely bovine serum albumin BSA by utilization of the activated ester method, and then obtains the bisphenol A-type medicine general artificial antigen, namely diphenolic acid-bovine serum albumin; and the coupling ratio of an coupling object is determined by an ultraviolet scanner. The method successfully synthesizes the artificial antigen of the BHPVA, has concise and effective synthetic procedures, can be completely used for immunoassay, provides a necessary artificial antigen for further research of people, and can meet the demand of domestic research of the artificial antigen.

The invention provides a micro-grid system based on VACON industrial inverters. The system comprises a power supply unit module containing a unified convertor device, a convertor module and a PLC-based central monitoring and scheduling module. The power supply module of the system comprises a wind power supply unit, a photovoltaic power supply unit and an energy storage power supply unit with two-way power flow. As all power convertor devices of the micro-grid system are based on the VACON industrial inverters having a secondary development function, the universality and maintainability of the power supply convertor device and the scalability and secondary development property of protection and control functions are achieved. The central monitoring and scheduling module achieves system operation state monitoring, fault handling and protection, stable operation control, and coordinated optimization scheduling of all the power supply units. The system provided by the invention is conducive to the control of distributed power supplies.

The invention discloses a lacustrine core sampling device for sediments in lakes with different depths. The lacustrine core sampling device comprises a handle rod, a drilling rod and a rotary core capturing device which are sequentially fixedly connected from top to bottom, wherein the rotary core capturing device comprises a sample pipe and a rotary blade, a sample pipe body is a semicircular groove body, a connecting rod connected with the drilling rod is arranged at the top end of the semicircular groove body, a drill bit is fixedly arranged on the outer side surface of the bottom end of the semicircular groove body, and two ends of the rotary blade are respectively rotatably connected to the top end and the bottom end of the semicircular groove body. According to the lacustrine core sampling device, the defect of an existing mechanical sediment sampler is overcome, the sampling success rate and the sample integrity are increased, lacustrine cores of water bodies and marshes in different depths are sampled through the arrangement of prolongable drilling rod and a hammer, the dismantling link is greatly simplified, the time is effectively saved, and the demand on research is also met.

The invention discloses a method for preparing alpha-AlH3 under the control of a diisobutylaluminum hydride crystal transformation assistant. The method comprises the following steps: 1, dissolving AlH3 etherate and diisobutylaluminum hydride in anhydrous toluene or benzene, stirring at 92-94DEG C for 2-3h until a reaction ends, and standing until room temperature; and 2, filtering to obtain a white reaction product, washing by using ether 2-4 times, washing by using dilute hydrochloric acid 1-2 times, washing by using deionized water 2-3 times, and carrying out vacuum drying at 40-90DEG C for 5-8h under a degree of vacuum of 5-20Pa to obtain a white powder product. The method allows the above raw material to react under the action of the diisobutylaluminum hydride crystal transformation assistant in order to generate high yield alpha-AlH3, and realizes no side reactions.

A flight parameter testing device of a micro-miniature flapping wing air vehicle comprises a stationary track unit, a flight parameter collecting unit and a flapping wing mechanism unit. The flight parameter testing device is simple in structure and high in automation degree, the micro-miniature flapping wing air vehicle is ingeniously and dynamically tested in the flight process, the flight parameter collecting unit and the flapping wing mechanism unit move on guide rails of the stationary track unit through a sliding table, the moving speed of the sliding table is controlled through a driving motor, in this way, flight states of the flapping wing mechanism at different wind speeds are achieved, all flight parameters of the flapping wing air vehicle can be accurately measured through a laser two-dimension scanning sensor and a six-axis force sensor, and reliable flight parameter testing data are provided for researching the flapping wing air vehicle. The laying length of the guide rails can be flexibly set, and all the flight parameters of the flapping wing mechanism at different pitch angles can be tested through replacing different wing types of wings made of different materials and adjusting the wing pitch angle of the flapping wing mechanism unit.

The invention provides a manufacturing method of a paraffin tissue chip, which is characterized by comprising the following detailed steps of: numbering the tissues needed, then impregnating paraffin or numbering after marking the tissues needed on the existing paraffin; cutting the tissues into needed size, putting the cut tissues on an ironing table, melting the paraffin thereof at the temperature of 60 to 65 DEG C to obtain tissues to be embedded; taking another paraffin into a paraffin pot, and using an electric furnace to heat and melt the paraffin; putting a square paraffin mold on a glass board, impregnating the tissues to be embedded obtained by the step 1 in the melten paraffin, arranging the tissues at one side of the inner part of the paraffin mold, recording the numbers of tissues of corresponding positions on a positioning board or a positioning paper, reserving a square vacant position at the other side of the inner part of the paraffin mold, pouring the melten paraffin into the paraffin mold along the vacant position till the tissues are impregnated, polymerizing the paraffin, removing the paraffin mold and cutting the obtained paraffin into pieces along the direction parallel to the glass board. The manufacturing method of the paraffin tissue chip has the advantages of cheap price, convenience, fastness, high yield and diverse material-obtaining and can be popularized into each pathology department and relevant research units.

The present invention discloses a system and method of vehicle cable terminal frequency domain dielectric spectrum experiment in an operation condition. The system comprises an experiment box body, and a cable terminal sample body, an insulation state detection unit, a cable terminal sample body, a frequency domain dielectric spectrum test system and a temperature regulation system 1 arranged in the experiment box body. The cable terminal sample body 21 is a cable terminal through processing, a hollow cylinder with small diameter is drilled out from the center of an internal cable core 31, and a resistance heating bar 32 passes through a hollow cylinder center and is connected with a radio-frequency power supply 6 through a wire. A function for simulation of temperature operation of cable in the load condition is realized. The obstacle caused by high cost and large size of the operation environment of a current phytotron simulation cable for the cable dielectric spectrum experiment is overcome so as to satisfy the research need, and the simple and practicable system and method of the vehicle cable terminal frequency domain dielectric spectrum experiment in the operation condition are provided.

The invention relates to a F-Ketamine hapten, a F-Ketamine antigen as well as a preparation method and application thereof. The structure of the F-Ketamine hapten is shown as a formula (I). The preparation method of the F-Ketamine hapten comprises the following steps: taking F-Ketamine and a compound shown as a formula (II) as raw materials, carrying out reacting, and introducing an active arm with carboxyl on carbonyl of the F-Ketamine to obtain the F-Ketamine hapten. Furthermore, after the F-Ketamine hapten is activated, the F-Ketamine hapten and carrier protein are subjected to a coupling reaction to obtain the F-Ketamine antigen. The invention initiates a novel F-Ketamine artificial hapten and a novel F-Ketamine artificial antigen for the first time; and the novel F-Ketamine artificialhapten and the novel F-Ketamine artificial antigen can be applied to an immunochromatography technology, have the advantages of high sensitivity, high specificity, simplicity, rapidness, easiness inoperation, no need of any large-scale instruments and equipment and the like, and can be used for large-scale preliminary screening of F-Ketamine.

The invention provides a sensor fault diagnosis method and system of a first-order follow-up system based on a dynamic trend, and relates to a sensor fault diagnosis method of a first-order follow-upcontrol system. The method includes steps: realizing switching between a multiplicative fault module, a non-fault module and an additive fault module through simulation of a mechanical switch, and acquiring experiment data; establishing fault monitoring, fault estimation and fault separation static models based on analysis of normal and fault system dynamic characteristics, and proposing an onlineapplication diagnosis model calibration method and a diagnosis process; and verifying the validity of the fault diagnosis method and high precision of the diagnosis according to online simulation ofa "complex process system innovation experiment platform". The method can be widely applied to sensor online fault diagnosis of the first-order follow-up control system.

The invention discloses paraquat hapten, paraquat complete antigen and a preparation method of the paraquat hapten and the paraquat complete antigen. Midbody 4,4-bipyridine of paraquat is utilized to combined analogue N-methyl-N'-valeric acid group-bipyridine-bibromide, carrier protein bovine serum albumin (BSA) is coupled through a mixed anhydride method, and paraquat artificial complete antigen can be successfully combined after ultraviolet spectrum identification and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) identification. The preparation method is simple and effective and low in cost. The combined paraquat artificial complete antigen can be applied to preparing peculiarly identifying efficient antibody of chemical pesticide paraquat and developing immune quick test kits remained by quick detection of paraquat to provide convenient methods for detection research of the materials.

A synthetic method for the general artificial antigen of a beta-lactam medicine belongs to the biochemical technical field. The invention adopts 6-amino penicillanic acid as hapten, couples the hapten with the carrier protein of bovine serum albumin BSA through an N-(m-maleimide group benzene methanoyl) succinimides MBS method, and determines the coupling ratio of the coupled matter through a gel electrophoresis method. The method successfully synthesizes the 6-APA general artificial antigen with a simple and effective synthesis process. The method which can completely be used for immune analysis provides necessary artificial antigen for future study and meets the requirements of domestic study on the artificial antigen.

The invention relates to an augmented reality system experiment platform applied to robot assisting surgical navigation. The augmented reality system experiment platform comprises a support main body (1), a horizontal working platform (2), a projector (3), binocular stereo vision cameras (4), auxiliary light sources (5), a height sensor (6) and a head mold fixing frame arranged on the horizontal working platform (2) and capable of rotating along the axial direction. The support main body (1) is in a cuboid framework type structure. The projector (3) projecting towards a position below is fixedly arranged in the center of the upper portion of the support main body (1). The binocular stereo vision cameras (4) are symmetrically fixedly installed at two sides of the projector (3), and the interval can be adjusted. The auxiliary light sources (5) with angles and brightness capable of being adjusted are installed on four corners of the upper surface of a framework. The horizontal working platform (2) capable of moving upwards and downwards is installed at a horizontal position of the middle of the support main body (1). The height sensor (6) is fixed on a side stand column of the support main body (1), is connected with the horizontal working platform (2), and is used for measuring the height value of the side stand column.

The invention discloses a suicide risk identification method based on a microblog text. The suicide risk identification method comprises the following steps: S1, establishing a suicide microblog corpus; S2, establishing a suicide microblog corpus recognition model; S3, carrying out experimental design to find an optimal model; S4, outputting a judgment result; The method has the beneficial effectsthat the user with the suicide risk can be quickly and actively identified by adopting the method, and effective intervention can be timely discovered and carried out at the early stage of individualsuicide idea formation, so that the coverage and the speed of suicide risk assessment work are greatly improved; The common problem of lack of suicide text data in previous research is solved; The bottleneck problem of improvement of prediction precision of a single structure of the current neural network is solved, and the method has good social benefits and economic benefits when applied to early prevention of suicide intervention.

The invention provides a collection and liquid-separation container and a method for measuring content of light hydrocarbons by the utilization of the collection and liquid-separation container. The collection and liquid-separation container comprises a housing, an internal cavity and a fluid communicating pipe. The housing comprises a cylindrical sidewall, an arc-shaped top cover and an arc-shaped bottom cap. The fluid communicating pipe contains a sample introduction tube, a gas product output tube and a liquid-separation and liquid-discharge tube. The sample introduction tube and the gas product output tube pass through and extent out of the arc-shaped top cover. The liquid-separation and liquid-discharge tube contains an upper capillary tube section, a variable diameter section and a lower liquid-discharge section. The upper capillary tube section is arranged on the outer wall of the arc-shaped bottom cap and is communicated with the internal cavity through a through hole on the arc-shaped bottom cap. According to the method for measuring content of light hydrocarbons by the utilization of the above collection and liquid-separation container, the collection and liquid-separation container is used for collection and measurement of volatile light hydrocarbons composition, and a capillary tube is used for low-temperature enclosed liquid-separation. Content of volatile components is measured at normal temperature and normal pressure in an open environment. Thus, content of volatile light hydrocarbons composition is accurately measured.

A synthesis method of an universal artificial antigen of alkylphenol medicines belongs to the technical field of biochemical engineering. The synthesis method is as follows: nonyl phenol (NP) is used as hapten, and the active ester method is adopted to couple the hapten and the carrier protein bovine serum albumin (BSA) to obtain the universal artificial antigen of alkylphenol medicines. Polyacrylamide gel electrophoresis is adopted to detect the coupling ratio of the conjugate. By adopting the method, the universal artificial antigen of alkylphenol medicines can be synthesized successfully; the synthesis step is concise and effective; and the method can be completely used in immunoassay, provide the necessary artificial antigen for the future research of people and meet the need of the domestic research on the artificial antigen.

The invention discloses a method for synthesizing an artificial antigen of melamine, pertaining to the technical field of biochemical industry. In the method, the melamine is taken as a semiantigen and the existing amino group in the structure thereof is utilized, the semiantigen is combined with bovine serum albumin through glutaral pentanedial, and the artificial antigen of melamine which is melamine-bovine serum albumin is prepared; the products prepared can be applied to the detection of content of melamine in foods and immunity, with advantages of fast detection, convenient operation and low cost. The method has concise and effective synthesis steps, thus being completely capable of being applied to immunity analysis, providing necessary artificial antigen for the future research, and meeting the requirement for the research thereon in China.

The invention discloses an ultraviolet and deep ultraviolet optical thin film element double-wavelength laser fluorescence spectrometer, which relates to the technical field of application of an optical thin film element. The spectrometer comprises an ArF laser excitation module, a KrF laser excitation module, a sample chamber, a fluorescence detection module and an experimental synchronous control module, wherein the ArF laser excitation module and the KrF laser excitation module emit and transmit laser; the laser is emitted into the sample surface of the sample chamber to produce fluorescence; the fluorescence generated by the excitation of a sample is emitted out of the sample chamber, is transmitted to the fluorescence detection module in an incidence way to carry out spectrum light split, and is detected by a photoelectric detector to convert a fluorescence signal into an electric signal; and the electric signal is transmitted to the experimental synchronous control module. In the spectrometer, two lasers of ArF laser and KrF laser are used as laser sources, so that the sensitivity of ultraviolet and deep ultraviolet fluorescence spectrum detection is improved, and the requirements of testing of an ultraviolet and deep ultraviolet waveband optical thin film weak fluorescence spectrum can be met.

The invention discloses a ray chromatography imaging method for refracted waves, belongs to a ray imaging technology, and mainly solves the problem of ray imaging of a refracting layer in an earthquake. The ray chromatography imaging method for the refracted waves comprises the following steps of: (1) performing data acquisition, namely acquiring earthquake travel time tm; (2) establishing a velocity model; (3) establishing a linear equation of inversion of the travel time; (4) solving a velocity increment according to the travel time inversion equation; and (5) modifying the velocity model by using the solved velocity increment, solving a new velocity increment according to a new travel time inversion equation, modifying the currently used velocity model by using the new velocity increment, and repeatedly modifying until the solved velocity increment meets an inversion norm, namely the ray chromatography imaging of the refracted waves is achieved at the moment. The method is simple, easy to operate, and high in imaging quality and efficiency.

A making method for a slide glass of large area includes the following steps: (1) cutting a plane with processing allowance close to a sample plane requiring observation; (2)polishing the plane successively by coarse sands and fine sands after being snapping at fixed-points; (3) sticking the polished sample plane on the slide glass by using crystal clear epoxy resin, eliminating bubbles by heating during the sticking operation and then solidifying; (4) cutting the excrescent sample on a section cutter with steps paralleling with the slide glass and remaining a thin sample slice with the thickness of millimeters on the slide glass; (5) further grinding the sample slice to be thinner on a slice grinder by manually holding the slide glass; (6) moving to a glass plate, and manually wearing the sample to the required thickness by fine sands, meanwhile, a microscope is required to repeatedly check the thickness of the sample. The invention, overcoming the difficulty of making a slide glass of large area, satisfies the researching need for large samples, and does not lose all required construction detail on the sample slice.

The invention relates to a hybrid cell line which can adapt to human HVB virus natural infection and replication and can be subcultured and a preparation method thereof. The hybrid cell line of HepChLine are obtained by fusion and hybridization of parent cells of human primary hepatocyte and negative HepG2 filtrated from HGPRT, which is new matter. The HepCHLine succeeds sensitivity of parental human hepatocyte by HBV infection and is characterized in that HepG2 can vaccination animal and subculture, which is not only suitable for natural infection but can be long - term subcultured. The invention, for the first time, prepares the hybrid cell lines of HepChLine, which are not only suitable for natural infection of human HVB virus, but can be subcultured. The invention meets requirements for research of host cells infected at the condition of HVB or nature.

The invention relates to a preparation method of an artificial antigen applied to tartrazine, belonging to the technical field of biochemical engineering. According to the invention, a derivative TAR of the tartrazine is prepared by taking ethyl succinyl chloride and 2, 2-dimethyl-1, 2-dioxane-4,6-dione as raw materials through four-step chemical reaction, and the TAR which contains no sulfonic acid group is used as a hapten and coupled with the carrier protein BSA or OVA by adopting an active ester method to prepare the artificial antigen for the tartrazine; the coupling rate of the conjugate is measured by adopting the spectrophotometry; the specific antibody which corresponds to the tartrazine is generated in an immunizing manner; and the enzyme-linked immunodetection method is established. The method successfully synthesized the artificial antigen of the tartrazine which can be fully used for immunoassay with concise and effective steps, thus providing necessary artificial antigen of the tartrazine for the further research of people, and meeting the domestic demand for the research of the tartrazine.