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Method for synthesizing general artificial antigen of beta-lactam drugs

A technology of artificial antigens and lactams, applied in the preparation methods of peptides, chemical instruments and methods, animal/human proteins, etc., to achieve the effect of concise synthesis steps

Inactive Publication Date: 2008-11-19
JIANGNAN UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the colloidal gold immunoassay method for penicillin antibiotics in China. In order to make up for this gap, a penicillin intermediate 6-aminopenicillanic acid was designed and synthesized for penicillin antibiotics. complete antigen for antibiotic detection

Method used

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  • Method for synthesizing general artificial antigen of beta-lactam drugs
  • Method for synthesizing general artificial antigen of beta-lactam drugs
  • Method for synthesizing general artificial antigen of beta-lactam drugs

Examples

Experimental program
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Embodiment 1

[0028] (1) Acylation of 6-aminopenicillanic acid

[0029] Dissolve 70umol 6-APA in 3ml PBS (0.05mol / L, pH 7.0), and add it to a 25ml beaker containing 70umol MBS (22mg) and 2ml tetrahydrofuran. The mixture was reacted at 37°C for three hours, freeze-dried to obtain a white solid, which was washed five times with diethyl ether and dichloromethane (2:1) to remove unreacted MBS.

[0030] MBS monitoring: for the production of silica gel thin-layer plates, weigh 12g of silica gel, dissolve it in 40ml of 0.5% mass concentration sodium hydroxymethyl cellulose solution, stir it fully with a glass rod to form a paste, oscillate ultrasonically for 1 minute, and spread it evenly. Spread it on a glass plate, put it in a cool place to dry naturally, put it in an oven at 105°C for 1 hour to activate, and finally put it in a drying oven for later use.

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Abstract

A synthetic method for the general artificial antigen of a beta-lactam medicine belongs to the biochemical technical field. The invention adopts 6-amino penicillanic acid as hapten, couples the hapten with the carrier protein of bovine serum albumin BSA through an N-(m-maleimide group benzene methanoyl) succinimides MBS method, and determines the coupling ratio of the coupled matter through a gel electrophoresis method. The method successfully synthesizes the 6-APA general artificial antigen with a simple and effective synthesis process. The method which can completely be used for immune analysis provides necessary artificial antigen for future study and meets the requirements of domestic study on the artificial antigen.

Description

technical field [0001] The invention relates to a method for synthesizing a general artificial antigen of β-lactam drugs, which belongs to the field of biochemical technology. Background technique [0002] 6-aminopenicillanic acid, English name: 6-aminopenicillanic acid (6-APA), CAS number: 551-16-6, molecular formula is C 8 h 12 N 2 o 3 S. β-lactam antibiotics refer to a class of antibiotics containing a β-lactam ring in their chemical structure, mainly including penicillins and cephalosporins, and their function is characterized by being able to inhibit the activity of bacterial mucopeptide transpeptidase, thereby preventing bacterial Synthesis of cell walls, exhibits bactericidal activity. In animal husbandry, β-lactam antibiotics are widely used to control mastitis in dairy cows, and treat animal urinary tract, gastrointestinal tract and respiratory tract infections. However, due to its improper use or non-compliance with the withdrawal period regulations, etc., it...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/10C07K14/765
Inventor 胥传来马伟彭池方徐丽广李灼坤
Owner JIANGNAN UNIV
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