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Synthesis method of universal artificial antigen of alkylphenol medicines

A technology of artificial antigens and alkyl phenols, applied in biological testing, animal/human proteins, material testing products, etc., to achieve the effect of concise synthesis steps

Inactive Publication Date: 2011-07-13
JIANGNAN UNIV +1
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  • Abstract
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Problems solved by technology

So far, there is no report on the immunological detection method for the residual structure of alkylphenols at home and abroad. Therefore, an immunoassay for the detection of the structure of alkylphenols was designed and synthesized using nonylphenol NP, the degradation product of alkylphenols, as a hapten. complete antigen

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  • Synthesis method of universal artificial antigen of alkylphenol medicines
  • Synthesis method of universal artificial antigen of alkylphenol medicines
  • Synthesis method of universal artificial antigen of alkylphenol medicines

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Embodiment 1

[0015] (1) Preparation of artificial antigen

[0016] Take 220.35mg (1mmol ) nonylphenol NP, 1.140g (10mmol ) glutaric anhydride, and 22.4mg (0.2 mmol ) lutidine DMAP into a round bottom flask, then add 9mL absolute ethanol and 1mL anhydrous pyridine , and finally placed in a 65°C water bath for 24h. The product solution was purified by vacuum rotary evaporation, and then dissolved in DMF solution for the next reaction. Take 0.5mL N,N-dimethylformamide DMF solution containing 10mg (0.04538 mmol) nonylphenol NP derivatives to a brown vial, then add 14.77mg (0.06807 mmol) N-hydroxysulfosuccinimide Sulf -NHS and 14.78mg (0.07715 mmol) carbodiimide EDC, reacted at room temperature for 6h, and it was liquid A. Dissolve 37.55mg (0.56725 μmol) of bovine serum albumin BSA into 0.01M, pH7.4 phosphate PBS buffer solution, which is solution B. Then slowly add solution A to solution B dropwise, react at room temperature for 3 hours, centrifuge to remove the precipitate, and take the su...

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Abstract

A synthesis method of an universal artificial antigen of alkylphenol medicines belongs to the technical field of biochemical engineering. The synthesis method is as follows: nonyl phenol (NP) is used as hapten, and the active ester method is adopted to couple the hapten and the carrier protein bovine serum albumin (BSA) to obtain the universal artificial antigen of alkylphenol medicines. Polyacrylamide gel electrophoresis is adopted to detect the coupling ratio of the conjugate. By adopting the method, the universal artificial antigen of alkylphenol medicines can be synthesized successfully; the synthesis step is concise and effective; and the method can be completely used in immunoassay, provide the necessary artificial antigen for the future research of people and meet the need of the domestic research on the artificial antigen.

Description

technical field [0001] The invention discloses a method for synthesizing a general artificial antigen of alkylphenol drugs, which belongs to the technical field of biochemistry. Background technique [0002] Alkylphenol compounds (Alkyl Phenol, APs) are an important kind of endocrine disrupting substances, which have obvious environmental estrogen effects. Most of these compounds are fat-soluble organic compounds with strong chemical stability. Once ingested, they are not easily degraded and excreted. They are bioaccumulative and highly harmful. Even a small amount can affect organisms. At the same time, it is widely distributed in the environment and its content is low. Alkylphenol compounds APs are important fine chemical raw materials, used in surfactants, lubricating oil additives, oil-soluble phenolic resins and insulating materials, textile printing and dyeing, paper-making auxiliaries, pesticide emulsifiers, industrial detergents, anti-aging of rubber and plastics A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/765G01N33/53
Inventor 王利兵胥传来李灼坤邢常瑞宋珊珊刘丽强
Owner JIANGNAN UNIV
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