System for identifying and evaluating seedling resistance of malus
A resistance identification and plant seedling technology, applied in the agricultural field, can solve the problem of no resistance identification and evaluation system, and achieve the effect of shortening the identification cycle and breeding years, reliable results and good repeatability.
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[0030] The present invention is described in detail below in conjunction with example:
[0031] Identification and evaluation of hypoxia tolerance in the rhizosphere of Malus plants:
[0032] 1. Materials and methods
[0033] 1.1 Test materials
[0034] Table 1 Test materials and sources
[0035]
[0036] 1.2 Seedling cultivation
[0037] Sow the layered seeds into a nutrient pot (12cm high x 12cm in diameter), cultivate on a sand base, 2 plants per pot, and spray 1 / 2 Hoagland nutrient solution once every 4 days when the seedlings grow 2 true leaves , supplemented with water in the meantime.
[0038] 1.3 Coercion treatment
[0039] 1.3.1 Hypoxic stress treatment
[0040] When the seedlings grow to 8 true leaves, select uniform seedlings and move them to 1 / 2 Hoagland nutrient solution hydroponic pots (20L), 6 pots of each material, 50 plants in each pot, arranged randomly. The air pump was used for normal ventilation, the pH of the nutrient solution was adjusted to 6....
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