The invention discloses waterborne biomass negative oxygen ion paint. A formula of the waterborne biomass negative oxygen ion paint comprises the following raw materials by weight: 45-60 parts of a pure natural plant extract, 25-40 parts of a mineral extract, 25-40 parts of filler, 15-30 parts of a surfactant, 5-10 parts of a tackifier, 1-3 parts of an antifreeze agent, 1-3 parts of a dispersion agent, 0.8-1.2 parts of a film-forming agent, 0.4-0.8 part of a defoaming agent and 0.2-0.8 part of a mildew preventive. According to the waterborne biomass negative oxygen ion paint, an extracted pure natural plant, the rare mineral extract and the like are used as the raw materials, thus the comprehensive performance of the biomass paint is good, a wall is air-permeable, negative oxygen ions escape in an infrared radiation mode, and active time of solid molecules is long so that the negative oxygen ions can be released for a long time. The waterborne biomass negative oxygen ion paint is high in wear resistance capability, scratch-resistant, high-temperature-resistant, acid and alkali resistant, high in stability and long in service life, has functions of removal of harmful gas VOCs (volatile organic compounds), formaldehyde decomposition, air purification, health care and the like, does not contain formaldehyde, and is suitable for large-scale popularization.