The invention discloses a medicinal combination for treating chicken pasteurellosis and a preparation method thereof. The medicinal combination is prepared from the following raw materials: rhizoma coptidis, radix scutellariae, radix et rhizoma rhei, radix gentianae, radix isatidis, honeysuckle flower, common andrographis herb, epimedium herb, mirabilitum dehydratum, flos magnoliae, herba taxilli, common selfheal fruit-spike, spreading hedyotis herb, rohdea japonica, semen pharbitidis, radix aconiti carmichaeli, folium isatidis, rhizoma belamcandae, herba artemisiae annuae, borax, plantain herb, distilled water, norfloxacin, enrofloxacin, penicillin, terramycin, sulfadiazine sodium, powered glucose, streptomycin, vitamin, kanamycin, calcium hydrophosphate, doxycycline hydrochloride and erythromycin thiocyanate. The medicinal combination is reasonable in component configuration, is scientific in preparation method, is prepared from traditional Chinese medicine and western medicine in an organic combination way, is easy to use, can effectively prevent and treat the chicken pasteurellosis, is low in infection rate, is high in survival rate, and is free from toxic and side effects.