112results about How to "No inflammatory response" patented technology

The invention provides an implanted flexible neural microelectrode comb, and a preparation method and an implanting method thereof. The flexible neural microelectrode comb is mainly composed of a flexible substrate layer, a flexible insulation layer and a metal connection wire layer arranged between the flexible substrate layer and the flexible insulation layer; the flexible neural microelectrode comb comprises a comb-like structure, a grid structure, a solid structure and a welding pad connected in sequence; electrode sites are arranged on the comb-like structure; welding points are arranged on the welding pad; the metal connection wire layer is composed of metal connection wires connecting the electrode sites and the welding points; and the flexible insulation layer is arranged on the surfaces of the electrode sites and the welding points. The flexible neural microelectrode comb prepared according to the method provided by the invention has a wire-grid-plane gradual changing structure, and thus is improved in mechanical stability during a shape changing process. The mechanical property of the implanted flexible neural microelectrode comb is matched with a brain tissue, the implanting areas is small, an inflammatory response of the brain is avoided, and electroencephalogram signals can be stably tracked and measured in a multi-point manner for a long time.

The invention provides marine biomaterial composite hydrogel dressing and a preparation method thereof and relates to the technical field of medical dressing. The hydrogel dressing is prepared from asodium alginate-fish collagen mixed solution and a chitosan-crosslinking agent mixed solution, wherein the sodium alginate-fish collagen mixed solution is prepared from sodium alginate, fish collagen,polyvinyl alcohol, sodium carboxymethyl cellulose, pectin, glycerin, propanediol and distilled water; the chitosan-crosslinking agent mixed solution is prepared from chitosan, a crosslinking agent, glacial acetic acid and distilled water. The invention also provides the preparation method of the dressing. The sodium alginate-fish collagen mixed solution is cultured at the constant temperature, the chitosan-crosslinking agent mixed solution is added, the mixture reacts, and the hydrogel dressing is obtained. The hydrogel dressing has excellent mechanical property, moisture retention and biocompatibility, accelerates wound repairing, can diminish inflammation and perform sterilization, has no wound inflammation reaction, effectively promotes wound healing, is easy to remove and does not stick to a wound surface.

The invention discloses a nano-hydroxyapatite / polyether-ether-ketone composite material and a bone repair body as well as a preparation method and application thereof. The preparation method of the composite material comprises the following steps: uniformly mixing 30-35wt% of 200nm-1mu m hydroxyapatite powder and 65-70wt% of 5-15mu m polyether-ether-ketone powder to obtain mixed powder; and processing the mixed powder and moulding to obtain the composite material. The composite material has good biocompatibility and bioactivity as well as mechanical properties matching the bone tissue, effectively prevents the stress shielding effect, and can stimulate bone growth, accelerate bone healing, shorten the healing time of the injury after material implantation of bone and reduce the probability of second operation. The preparation technology of the composite material can be adjusted to prepare bone repair bodies different in shape, specification and mechanical properties to meet the clinical needs. Inflammatory reaction is avoided after the implantation of the bone repair bodies, the mechanical properties are matched with human bone, negative effects such as bone repair material loosening and bone resorption are prevented, and the clinical needs for bone repair can be met.

The invention discloses bioglass / polyether-ether-ketone composite materials, a method for preparing the bioglass / polyether-ether-ketone composite materials, the application of the bioglass / polyether-ether-ketone composite materials, a bone repair body and a bone repair body preparation method. The composite material preparation method comprises the steps that 20-40wt% of 5-20-micron bioglass powder and 60-80wt% 10-20-micron polyether-ether-ketone powder are evenly mixed to obtain mixed powder; the mixed powder is processed and formed, and accordingly the composite materials are prepared. The composite materials have good biological activity and biocompatibility, the composite materials and bone tissues have good mechanical compatibility, and the composite materials can stimulate bone growth, accelerate bone cure, reduce cure time after bone repair materials are implanted, and have bacterium resistance performance. The preparation techniques of the composite materials can be adjusted according to clinic requirements to prepare bone repair bodies of different shapes, difference specifications and different mechanical properties. The bone repair body will not cause inflammatory responses after being implanted, the mechanical property of the bone repair body is matched with human bones, the negative effects that the bone repair materials loosen and bone resorption happens will not be caused, and the bone repair body can meet requirements of clinic for bone repair.

The invention provides a medical sodium alginate gel microsphere and a preparation method and application of the medical sodium alginate gel microsphere. The medical sodium alginate gel microsphere consists of a composite medicine carrier and a water-insoluble medicine; the medicine is coated with the composite medicine carrier; and the composite medicine carrier is an ion crosslinking agent-sodium alginate-divalent metal ion, wherein the ion crosslinking agent is 4-aminomethylbenzoic acid or tranexamic acid. The preparation method comprises the following steps of: (1) mixing ion crosslinking agent aqueous solution with divalent metal ion solution in the same volume to obtain composite solidifying liquid; (2) dispersing medicine powder or an agent into sodium alginate aqueous solution; uniformly mixing; dropwise adding the mixture into the composite solidifying liquid obtained in step (1) through a high-voltage static droplet generating device or a syringe needle, so that the mixture drops are solidified into spheres; and (3) dehydrating gel microspheres which are washed with the distilled water; and drying at normal temperature. The medical sodium alginate gel microsphere can be used for treating tuberculosis, endocrine disease and tumor, and also can be used for treating local acute hemorrhage and chronic errhysis.

The invention relates to the technical field of bone tissue engineering, and discloses a step-by-step tissue engineering bone building method. The method comprises the following steps of: (1) stent repair and pre-vascularization: building a tissue engineering bone sent at a bone defect part, and performing pre-vascularization; (2) later embedding of bone tissue engineering seed cells: after inflammatory reaction stage, embedding the seed cells, subjected to osteogenic induction, into the tissue engineering bone sent at the bone defect part based on growth-in time of capillaries, wherein the later embedding of bone tissue engineering seed cells in the step (2) is carried out 7-14 days later after the stent repair and pre-vascularization in the step (1). In the method provided by the invention, the sent promoting growth of vessels is firstly embedded, the seed cells for osteogenesis are embedded later, thus, a great segment of tissue engineering bone can be built quickly, application amount of the bone tissue engineering seed cells needed for repairing per unit volume of bone tissue is reduced greatly, and utilization rate of the seed cells is improved.

The invention discloses an oil-in-water type compound vaccine adjuvant and a method for preparing the same. The oil-in-water type compound vaccine adjuvant comprises an oil phase and a water phase, wherein the oil phase part comprises vitamin E, lecithin, propolis, mineral oil and a surface active agent; and the water phase part comprises an aqueous carrier, wherein the aqueous carrier comprises phosphate buffer solution and a hydrophilic surface active agent. The oil-in-water type compound vaccine adjuvant has the advantages of lowering viscosity of inactivated vaccines, making the vaccines more easily injected, making animals quickly generate immunoprotection with little local response, along with simple preparation method, low cost and the suitability for mass production of the vaccines.

The invention discloses a CS (calcium silicate) / PEEK (poly(ether-ether-ketone)) composite bone repair material, a bone repair body as well as a preparation method and an application of the material. In the CS / PEEK composite bone repair material, CS accounts for 30%-50% and PEEK accounts for 50%-70%; the particle size of CS is 20-30 mu m, and the particle size of PEEK is 30-40 mum. The CS / PEEK composite bone repair material has excellent mechanical properties, has the elasticity modulus similar to that of natural bone, has better osteogenesis bioactivity, has excellent mechanical compatibility, cannot cause bone resorption due to stress shielding, can promote regeneration of peripheral bone tissue and fusion with natural bone tissue after being implanted into a human body and can meet requirements of immediate fixation and long-term stability of postoperative orthopedic instruments.

The invention discloses a doxorubicin hydrochloride light-controlled sustained-release liquid crystal gel preparation and a preparation method thereof. The light-controlled sustained-release liquid crystal gel preparation is prepared from 15-40 wt% of phospholipid, 60-70 wt% of glyceride, 1-20 wt% of a cosolvent, 0.01-2 wt% of doxorubicin hydrochloride and 0.001-0.2 wt% of a photosensitizer. The doxorubicin hydrochloride light-controlled sustained-release liquid crystal gel preparation has the advantages of low viscosity, good fluidity, easiness in injection, rapidness in formation of the liquid crystal gel after being injected into or around a tumor or being placed in a post-operative tumor cavity, and realization of in-situ slow release of doxorubicin hydrochloride; and lights cause phase change of the liquid crystal gel and increase the drug release rate, and the preparation prolongs the drug action time, reduces the administration frequency, reduces the toxic and side effects of the drug, releases the drug as needed by adjusting the release of the drug through adjusting the illumination frequency or time according to the tumor pathological conditions of a patient, and greatly improves the bioavailability of the drug.

The invention discloses a fluorapatite / barium titanate / polyetheretherketone composite material as well as a preparation method and application of the composite material. The preparation method comprises the following step of: carrying out melt blending and extrusion granulating on fluorapatite, barium titanate and polyetheretherketone, wherein the use amount of fluorapatite is 10-30wt%, the use amount of barium titanate is 10-40wt%, and the use amount of polyetheretherketone is 50-60wt%. The invention also discloses a bone repairing body and a preparation method of the bone repairing body. The composite material has excellent biological activity and biocompatibility, and better mechanical compatibility with bone tissue, and can stimulate bone growth, accelerate bone healing and reduce the healing time of the material is implanted to the bone. The preparation method is simple and feasible in process. The bone repairing body has excellent biocompatibility, biological activity, bone mechanical compatibility and microorganism resistance, thereby shortening the bone healing time, and is high in strength, good in fatigue resistance and corrosion resistance and long in service life, thereby meeting the clinical bone repairing requirements.

The invention discloses a rapid hemostatic hydrogel. The rapid hemostatic hydrogel comprises acrylamide, inorganic nanoparticles, tannic acid, a crosslinking agent, an initiator and an accelerant, wherein the addition amount of the inorganic nanoparticles is 0.2-10% of the weight of the acrylamide; the addition amount of the tannic acid is 1-20% of the weight of the acrylamide; the addition amountof the crosslinking agent is 0.25-2% of the weight of the acrylamide; the addition amount of the initiator is 0.5-3% of the weight of the acrylamide; 20-40[mu]L of the accelerant is added into each 100g of the acrylamide. The rapid hemostatic hydrogel has the advantages as follows: 1, the rapid hemostatic hydrogel with wet tissue adhesion, prepared by a preparation method provided by the invention, can be firmly adhered to a body tissue without relying on an additional adhesive, an additional bandage and the like during using, can maintain good adhesion strength during repeated use, and is very easy to strip; 2, the hemostatic hydrogel material has relatively high mechanical strength, has the elongation at break as high as 2200% and the compressive strength of 10.15MPa plus or minus 0.36MPa, and can meet surgical requirements.

The invention discloses a mesoporous calcium magnesium silicate and polyetheretherketone composite, bone prosthesis as well as a preparation method and application of the composite. The preparation method of the mesoporous calcium magnesium silicate / polyetheretherketone composite comprises the following steps: uniformly mixing 20-40wt% of mesoporous calcium magnesium silicate and 6-80wt% of polyetheretherketone composite of 10-20microns so as to obtain mixed powder; mixing the mixed powder with absolute ethyl alcohol, ultrasonically dispersing, and evaporating the absolute ethyl alcohol at 58-62 DEG C so as to obtain composite powder; and pressing and molding the composite powder by virtue of a mould pressing method so as to obtain the composite. The mesoporous calcium magnesium silicate is uniform in particle size and is uniform in mesoporous pore size distribution. The composite is good in bioactivity and biocompatibility, relatively good in mechanical compatibility with bone tissue, and capable of stimulating bone growth, accelerating bone healing and shortening healing time of bone after implanting material. The bone prosthesis is good in bone mechanical compatibility and antibacterial property, high in strength, fatigue-resistant, good in corrosion resistance and long in service life.

The invention discloses a preparation method of a pelvic floor patch composite biological material. The preparation method comprises the specific step of treating accellular matrix gel and a polypropylene material at pressure of at least 100MPa for at least 5 minutes. The preparation method is simple, enables the accellular matrix gel and the polypropylene material to be combined firmly, does not utilize chemical crosslinking agent in a crosslinking process, and is free from chemical residuals. The invention also discloses the pelvic floor patch composite biological material prepared by the method, and application of the material. Animal experiments prove that the material has the advantages of being good in tissue compatibility and being free from inflammatory response and can be used for treating pelvic organ prolapse.

The invention discloses polysaccharide-based hemostasis sponge with an initiative hemostasis function as well as a preparation process and application thereof. The prepared hemostasis sponge can be used for bleeding and diffusive bleeding of a body surface and organs inside the body. When the polysaccharide-based hemostasis sponge contacts blood, an initial hemostasis method and a passive hemostasis method can be simultaneously adopted to accelerate the hemostasis process, and for different bleeding types, the hemostasis effect is significant. The polysaccharide-based hemostasis sponge has good biological compatibility and blood compatibility, excellent biological security, biodegradability and the like and has a promising clinical application prospect.

The invention belongs to the field of biomedical engineering, and relates to a human tissue-engineered cardiac muscle tissue and a preparation method thereof. The human tissue-engineered cardiac muscle tissue disclosed by the invention is prepared by inoculating all kinds of cell components of a normal heart differentiated from human induced multipotential stem cells by taking a natural decellularized heart substrate as the scaffold. The human tissue-engineered cardiac muscle tissue has cell components, extracellular matrixes and biological functions similar to that of a normal human cardiac muscle tissue; testing and screening of in-vitro drugs, initial in-vivo transplantation researches of clinical earlier-stage small animals and observation of treatment effects can be carried out; and helps can be finally offered for realizing individualized treatment of human cardiovascular diseases.

The invention discloses a polypeptide microneedle and a preparation method thereof. The polypeptide microneedle comprises a base and a needle body fixed to the base, wherein the base is made of a high polymer material, and the high polymer material is selected from polyvinyl alcohol, polycarboxymethyl cellulose, chondroitin sulfate, polylactic acid-polyglycolic acid copolymer, fibroin, dextrin, hyaluronic acid or gelatin; and the needle body is prepared from the following raw materials: bioactive beautifying polypeptide, hyaluronic acid and deionized water. The microneedle is simple in preparation method, low in cost and suitable for mass production, can be widely applied to the field of cosmetic polypeptide, and has the excellent effects of removing wrinkles, resisting ageing, removing acnes, repairing the skin, whitening the skin, removing freckles, relieving the skin, resisting allergy, removing under-eye puffiness or promoting hair growth and strengthening hair.

The invention belongs to the technical field of construction of artificial organs by a tissue engineering method in biomedical engineering and relates to a tissue engineered breast transplant and a constructing method thereof. The tissue engineered breast transplant of the invention takes injectable material I type collagen gel as a carrier bracket and takes adipose-derived stem cells as seed cells; the seed cells are attached to the carrier bracket to form an adipose-derived stem cell / I type collagen gel compound; the final concentration of collagen is 2 mg / ml; and the cell density is 2*10<7> / ml. The tissue engineered breast transplant of the invention consists of injectable material-loaded autologous adipose-derived stem cells and is simple and convenient to prepare. Experiments prove that a new adipose tissue can be produced and the tissue engineered breast transplant is finally converted into autologous fat without an immunological rejection problem, so a new solution is provided for the filling and reconstruction of a breast.

A human anti-HBsAg mono-chain antibody / human antibody light chain constant region / protamine truncate recombinant gene, encoding protein and use are disclosed. The protein has antigen and nucleic acid combined activities. The process is carried out by: combining HBV siRNA with siRNA expression plasmid, transferring to HBV infectious cell specifically, and inhibiting HBV gene expression and copy. It has excellent specificity, efficiency, continuity and safety.

The invention relates to a preparation method of a mulberry silk tissue engineering scaffold. Artificially synthesized macromolecular material PLGA (polylactic-co-glycolic acid) is adopted in the traditional method, degradation products of the PLGA are acid, local pH value is reduced and inflammation can be easily caused. The preparation method provided by the invention comprises that mulberry silk is taken as a raw material and is dissolved with CaCl2 solution with the mass fraction of 40% after being refined by Na2CO3 solution with the concentration of 0.5g / dL, then filtering is carried out by adopting a dialysis bag, the obtained fibroin protein solution is added into mixed solution of chitosan / acetic acid, stirring to be uniform is carried out, then mixture is added into a charging barrel of a sprayer of forming equipment, low-temperature forming is carried out, a freezing scaffold is placed into a freeze drying machine, freeze drying is carried out, and a scaffold which is in solid state at normal temperature is obtained. The degradation products of the mulberry silk tissue engineering scaffold prepared by the preparation method provided by the invention are polypeptides, local pH value can not be reduced, and inflammation can not be caused, thus the mulberry silk tissue engineering scaffold is harmless to human body.

The invention relates to a uterine cavity implant as well as a preparation method and application thereof. The uterine cavity implant is characterized by having a bagged structure. The uterine cavity implant comprises a substrate material of animal small intestine submucosa subjected to decellularization treatment. An implantable medical device for preventing and treating intrauterine adhesion comprises a biological tissue substrate material. The present decellularization technology for a biological repairing material is improved; compared with the present product, the biological tissue substrate material provided by the invention has the advantages of lower DNA residual quantity, lower immunogenicity, higher anti-infection capacity and higher repairing capacity; and the uterine cavity implant is beneficial to the recovering of tissues, such as, uterine cavity base layer, submucosa and mucous layer. Besides, the invention prepares the biological repairing material into the uterine cavity built-in structure which is used for isolating wound surfaces, repairing uterine cavity tissues and reducing scar forming, so that the intrauterine adhesion problem is solved, and the accordingly generated sterility problem also can be solved.

The invention discloses a hemostat, comprising gelatin, microbial transglutaminase zymogen, and an activating agent of the microbial transglutaminase zymogen. The ratio of gelatin to microbial transglutaminase zymogen to the activating agent is 100000:1-200:1-100. The invention also discloses a preparation method and an application of the hemostat. The hemostat provided by the invention has better stability and hemostatic effect, and enhanced safety and reliability.

The invention discloses a preparation method of spherical crystal calcined bone. The preparation method comprises the following steps: firstly, taking an allogeneic bone, removing soft tissue, performing coarse cutting, soaking in a NaOH solution and an H2O2 aqueous solution to remove immunogen and part of impurities, then performing primary low-temperature calcination, soaking in a mixed solution of sodium tripolyphosphate and dicalcium phosphate after calcination, and finally performing secondary high-temperature calcination. The spherical crystal calcined bone prepared by the method has higher compressive strength and breaking strength, does not generate inflammatory response when being implanted into a body, and has high biocompatibility. According to the invention, a secondary calcination technology is adopted, and a key step of adding a mixed solution of sodium tripolyphosphate and dicalcium phosphate to soak is adopted in the secondary calcination, so that the prepared material is enhanced in strength, improved in biocompatibility and suitable for bone transplantation.

The invention relates to the technical field of medical preparations and particularly relates to a preparation method of a collagen-tussah silk fibroin composite bracket loaded with BMSCs (bone marrowmesenchymal stem cells). The preparation method of the collagen-tussah silk fibroin composite bracket loaded with BMSCs comprises the following steps: step 1, preparing a collagen solution; step 2, preparing a tussah silk fibroin solution; step 3, preparing a suspension of the BMSCs; and step 4, preparing the composite bracket. The prepared collagen-tussah silk fibroin composite bracket loaded with the BMSCs is good in biocompatibility, free of inflammatory reaction, toxic reaction, immunological rejection reaction and the like, strong in cell adhesion and capable of promoting the repair of askin wound; and furthermore, the composite bracket can keep the humidity of the wound to prevent loss of body fluid and moisture, can provide a biological barrier to prevent wound infection and bacterial invasion, and has certain flexibility and tensile property to keep the functions and form of the composite bracket during tissue healing.

The invention provides medical dressing capable of promoting wound healing. The medical dressing is prepared from the following raw materials in parts by weight: 100 to 200 parts of gelatin, 50 to 100parts of sodium alginate, 80 to 160 parts of polylactic acid and 20 to 150 parts of mannose. According to the medical dressing capable of promoting the wound healing, all the raw materials are high molecular materials with good biological compatibility; the polylactic acid is added, so that the obtained medical dressing has an anti-inflammatory effect and has a good effect of promoting the woundhealing.

The invention provides a device for controlling animal follicle development and preventing and treating reproductive tract inflammation and a preparation method thereof. The device is internally provided with a nylon support, and a sustained-release material layer with estrogen, progestational hormones and antibacterial peptide is wrapped around the outer part of the support. The device is provided with a connecting part and two long arms, the bottom ends of the two long arms are connected to the connecting part, a certain included angle is formed between the two long arms, and the portions, at the long arms, of the sustained-release material layer protrude to form a plurality of sheet structures which are orderly arranged. According to the device and the preparation method thereof, medicines and silicone rubber raw materials are mixed according to a certain ratio, and after low-temperature vulcanization forming is carried out, the medicines combined with silicone rubber and a high polymer material support are prepared into a special vaginal embedding bolt; the medicines combined with the silicone rubber can be slowly released for a long time (10 days or more), so that the effectsof effectively controlling the animal follicle development and preventing and treating the vagina and uterine inflammation can be achieved; the adopted materials are non-toxic, harmless and smooth andsoft in texture and have a good compatibility with animal tissues; and the pick-and-place operation of the device is simple.

The invention discloses a mesoporous magnesium silicate / hydroxyapatite / polyether-ether-ketone composite material, a bone restoration and a preparation method and application thereof. The preparation method of the mesoporous magnesium silicate / hydroxyapatite / polyether-ether-ketone composite material comprises the steps that 15 wt%-20 wt% mesoporous magnesium silicate, 10 wt%-15 wt% hydroxyapatite and 65 wt%-75 wt% polyether-ether-ketone are uniformly mixed to obtain mixed powder; then the mixed powder is mixed with anhydrous ethanol, ultrasonic dispersion is conducted, and then the anhydrous ethanol is evaporated at the temperature ranging from 65 DEG C to 80 DEG C to obtain composite powder; a mold pressing method is adopted to conduct machining-shaping on the composite powder to obtain the composite material. The composite material is good in biological activity and biocompatibility and good in mechanical compatibility with bone tissues, and the composite material can stimulate bone growth, speed up bone healing and reduce the healing time after the material is implanted into the bone. The bone restoration has the advantages of being good in bone mechanical compatibility and antibacterial property, high in strength, good in fatigue resistance and corrosion resistance and long in service life.

The invention discloses an antibacterial fibroin-based bionic coating and a preparation method thereof. The bionic coating is prepared from the following raw materials: fibroin, caffeic acid dopamine, dopamine hydrochloride, chitosan fibers, polyurethane, boric acid, calcium metaphosphate, strontium fluorapatite, a seaweed extract, galuteolin, puffball powder, zinc oxide, strontium gluconate, glycine fumarate, potassium hydroxide, polyvinyl alcohol, distilled water, glycol salicylate and ethanol. The bionic coating provided by the invention is high in bonding intensity with a metal implant, low in coefficient of wear on a steel ball and excellent in wear resistance, and the bionic coating is excellent in antibacterial effect on staphylococcus aureus; and meanwhile, the bionic coating is good in biocompatibility, free from cell toxicity, free from formation of blood coagulation and thrombosis and free from any inflammatory reaction, and the bionic coating has a good clinical application value.

The invention discloses an elastin-like silk fiber porous composite material. The preparation raw materials of the porous composite material comprise elastin-like polypeptide and silkworm cocoons. A preparation method comprises the concrete steps that silkworm cocoons are cut up; degumming is performed to obtain silk fiber; the elastin-like polypeptide and the silk fiber are subjected to crosslinking to prepare the porous composite material. The composite material can be easily processed and formed; after cobalt 60 irradiation sterilization, the material performance is not influenced. The composite material has no influence on peripheral tissues, and can be melted into a whole with the peripheral tissues; meanwhile, the degradation rate of the composite material is matched with the bone or cartilage regeneration speed; the support effect can be continuously and stably provided for the cell growth.

The invention discloses novel application of a natural silk sericin-free silk fibroin core to preparation of a medical surgical instrument for human bodies. The natural silk sericin-free silk fibroin core has high mechanical property, high flexibility and an appropriate degradation speed. As proved by an in-vivo experiment on an animal, the natural silk sericin-free silk fibroin core is immune to exclusive reactions and toxic reaction, and is a good material for preparing various in-vivo medical instruments.

The invention discloses modified gelatin based composite sponge and a preparation method and application thereof. The modified gelatin based composite sponge is prepared through modified gelatin, polysaccharide, a crosslinking agent polyethylene glycol derivative, a buffer salt solution and the balance water, wherein the modified gelatin is side chain sulfhydrylation modified gelatin, and the chemical structure of the modified gelatin is shown in formula (I) or (II); the crosslinking agent polyethylene glycol derivative is a polyethylene glycol derivative with sulfydryl reaction activity and is used for crosslinking sulfydryl of the modified gelatin; the polysaccharide comprises hyaluronic acid, chiton, chondroitin sulfate, heparin, alginic acid and a derivative thereof; the buffer salt solution is a phosphate buffer solution with pH of 5.0-8.0. According to the modified gelatin based composite sponge prepared through the method, the inflammatory response caused by a crosslinking agentand residual functional groups fallen off after crosslinking can be avoided; the biocompatibility is improved; massive modified gelatin based composite sponge can be quickly and efficiently prepared.The invention also provides a medical-modified gelatin based composite sponge and a preparation method thereof.