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Elastin-like silk fiber porous composite material and application thereof

A porous composite material and elastin-like technology, which is applied in the field of biomedical materials, can solve the problems of small pore size, unfavorable cell tissue ingrowth, biological inertness and non-degradability, which are difficult to shape and limit applications, and achieve porosity. high effect

Active Publication Date: 2017-05-10
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, a variety of materials for bone and cartilage tissue engineering have emerged, such as electrospun polymer materials, which have the advantage of constructing micro or nano topological structures similar to extracellular matrix, but the material has a small pore size, which is not conducive to Cells enter the interior of the material thereby limiting tissue ingrowth
Secondly, there are hydrogel materials, which can facilitate the embedding of cells, but their biological inertness, non-degradability and difficulty in shaping limit its application

Method used

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  • Elastin-like silk fiber porous composite material and application thereof
  • Elastin-like silk fiber porous composite material and application thereof
  • Elastin-like silk fiber porous composite material and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0036] This embodiment provides a preparation method of elastin-like silk fiber porous composite material, the specific steps are:

[0037] Cut B.mori cocoons into pieces and dry them, store them in a desiccator, use deionized water as a solvent, prepare 1.9M sodium carbonate solution, heat to 100°C, soak the dried cocoon pieces in boiling sodium carbonate solution After taking it out, ultrasonically clean it 3 times with deionized water (10 minutes / time), dialyze with deionized water to remove salt, and then dry to obtain silk fibers (or you can also cut B.mori cocoons into pieces and place them in deionized In deionized water, after boiling for 0.5 to 24 hours under the condition of higher than atmospheric pressure, the silk fibers are taken out and dried and stored);

[0038]10 mg of freeze-dried silk fibers were immersed in 1 mL of elastin-like protein solution with a mass concentration of 1% and freeze-dried to shape, which was designated as S-ELP. After that, S-ELP was c...

Embodiment 2

[0040] This embodiment provides a preparation method of elastin-like silk fiber porous composite material, the specific steps are:

[0041] Cut B.mori cocoons into pieces and dry them, store them in a desiccator, use deionized water as solvent, prepare 1M hydrochloric acid solution, heat to 100°C, soak the dried cocoon pieces in boiling hydrochloric acid solution for 24 hours , after taking it out, use deionized water to ultrasonically clean it 3 times (10 minutes / time), use deionized water to dialyze and desalinate and then dry to obtain silk fiber;

[0042] Submerge 10 mg of freeze-dried silk fiber in 1 mL of elastin-like protein solution with a mass concentration of 0.01%, freeze-dry and form it, then immerse it in a genipin solution with a mass concentration of 0.01%, and react at 4°C for 48 hours to obtain a genipin cross-linked composite material (recorded as S-ELP-Genipin), washed with deionized water for 3 times, vacuum-dried, and sealed at 4°C.

Embodiment 3

[0044] This embodiment provides a preparation method of elastin-like silk fiber porous composite material, the specific steps are:

[0045] Cut B.mori silkworm cocoons into pieces and dry them, store them in a desiccator, use deionized water as solvent, prepare 0.01M sodium hydroxide solution, heat to 100°C, soak the dried silkworm cocoons in boiling hydroxide Sodium solution for 30 minutes, after taking it out, use deionized water to ultrasonically clean it 3 times (10 minutes / time), use deionized water to dialyze and desalinate and then dry to obtain silk fiber;

[0046] Submerge 10 mg of freeze-dried silk fiber in 1 mL of elastinoid solution (prepared with deionized water) with a mass concentration of 1% and lyophilize it, denoting it as S-ELP, and immerse the lyophilized S-ELP in 2% proanthocyanidin In the solution (prepared with deionized water), the proanthocyanidin cross-linked composite material (S-ELP-PA) was obtained after reacting at 37°C for 0.5 hours, washed with ...

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Abstract

The invention discloses an elastin-like silk fiber porous composite material. The preparation raw materials of the porous composite material comprise elastin-like polypeptide and silkworm cocoons. A preparation method comprises the concrete steps that silkworm cocoons are cut up; degumming is performed to obtain silk fiber; the elastin-like polypeptide and the silk fiber are subjected to crosslinking to prepare the porous composite material. The composite material can be easily processed and formed; after cobalt 60 irradiation sterilization, the material performance is not influenced. The composite material has no influence on peripheral tissues, and can be melted into a whole with the peripheral tissues; meanwhile, the degradation rate of the composite material is matched with the bone or cartilage regeneration speed; the support effect can be continuously and stably provided for the cell growth.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to an elastin-like silk fiber porous composite material and its application in preparing bone and cartilage scaffold materials for tissue engineering. Background technique [0002] Tumor resection, trauma, and congenital malformations are the main causes of bone and cartilage defects clinically. At present, the gold standard for repairing bone and cartilage defects is autologous tissue transplantation, but autologous tissue transplantation will cause secondary trauma to patients, and the amount of materials obtained is limited. Therefore, the repair of bone and cartilage defects is still a difficult point in the medical field. Tissue engineering (tissue engineering, TE) technology provides an effective method for the repair of bone and cartilage tissue defects. The basic principle of TE is to obtain a small amount of cells (also known as seed cells) from the body, culture and ex...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/22A61L27/56A61L27/58
CPCA61L27/227A61L27/365A61L27/3654A61L27/56A61L27/58A61L2430/02A61L2430/06
Inventor 陈富林陈卓玥李红民谢鑫崔继红余源张婧
Owner NORTHWEST UNIV
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